Izinhlobo ezi-4 zamagciwane okuphefumula
Igama lomkhiqizo
I-HWTS-RT099- 4 Izinhlobo zama-virus okuphefumula ikhithi yokuthola imvume ye-nucleic acid (Fluorescence PCR)
I-Epidemiology
Isifo segciwane leCorona, okubizwa ngokuthi "yi-Covil-19", kubhekiswa ku-pneumonia okubangelwa yi2019-nCoVUkutheleleka.2019-nCoVi-coronavirus ingeyohlobo lwe-β. I-COVIIG-19 yisifo esithelelekayo sokuphefumula esinamandla, futhi inani labantu livame ukwenzeka. Njengamanje, umthombo wokutheleleka uyisiguli kakhulu esithelelekile ngo2019-nCoV, futhi abantu abanegciwane le-asymptomatic batheleleke nabo bangaba ngumthombo wokutheleleka. Ngokusekelwe ophenyweni lwezempi lwamanje, isikhathi sokufakwa kwezinsuku eziyi-1-14, iningi lezinsuku ezingama-3-7. Umkhuhlane, ukukhwehlela owomile nokukhathala kungukubonakaliswa okuyinhloko. Iziguli ezimbalwa zazinezimpawus njengeUkuxinana kwamakhala, ikhala eligijimayo, umphimbo obuhlungu, i-myalgia kanye nohudo, njll.
Insungubezi
| -Yenza | Ngo-2019-n-Yivanhla |
| I-VIC (Hex) | I-RSV |
| Cy5 | IFV a |
| Ivupho | IFV B |
| Ind | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | -18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-9 |
| Uhlobo lwe-Specimen | I-Oropharyngengeal Swapy |
| Ct | ≤38 |
| Indlu edayisa insimbi | Ngo-2019-NCOV: 300Copies / MLUmkhuhlane virus / umkhuhlane b virus / ukuphefumula kwe-syncytial virus: 500copies / ml |
| Ukucacisa | a) Imiphumela yokusebenzisa kabusha i-Cross-Recivity ikhombisa ukuthi akukho ukusabela kwesiphambano phakathi kwekhithi ne-coronavirus sarsr-cov, i-HCOV-OCU1, HCOV-NL63, HCOV-NL63, 2, 3, Rhinovirus A, B, C, Chlamydia Pneumoniae, Metapneumovirus lomuntu, I-Enterovirus A, B, C, D, Igciwane le-Human pulmonary, igciwane le-epsterin-barl, igciwane le-mordetegalo, i-abuemophilus virus, i-haemophilus yomkhumulo I-Pneumoniae, i-streptococcus pyogene, i-mycobactella tuumoniae, i-mycobactelium tueberculosis, i-asperidus ye-aspergillus, ama-allidani e-candida Glabrata, i-pneumocystis jiroveci ne-cryptococcus entsha ne-genomic nucleic acid. b) Ikhono lokulwa ne-anti-anference ), i-beclomethasone (20mg / ml), dexamethasone (20mg / ml), I-Flunesisolide (20Cinolone Acetonide (2mg / ML), i-Budesonide (2mg / ML), i-MomeTasone (2mg / ml), i-fluticasone (2mg / ml), i-arpha interferoon (800IU / ML ), zanamivir (20mg / ml), i-ribavirin (10mg / ml), i-oseltamivir (60ng / ml), i-peramivir (1mg / ml), i-ritonavir (60mg / ml), i-mupirocin (20mg / ml), i-azithromycin ( I-1mg / ml), i-CEFTRIAXOne (40Cing / ML), iMeropenem (200mg / ML), i-levefloxacin (10μg / ml) kanye ne-tobramycin (0.6mg / ML) ukuze kuhlolwe amandla okuphazamisa, futhi imiphumela ibonisa ukuthi ukuguquguquka okushiwo ngenhla kwemiphumela yokuhlolwa ama-pathogen. |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 uhlelo lwe-real-time PCR Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®5 Systems real-time PCREMS |
Ukugeleza komsebenzi
Inketho 1.
I-Macro & Micro-Test Viral DNA / RNA KIT (HWTS-3004-32, HWTS-3004-48, HWS-3004-96) kanye ne-Macro & Micro-3004-9 (HWS-3006) Ekhiqizwe yiJiangsu Macro & Micro -Test Med-Tech Co, Ltd. Ivolumu yesampula ekhishwe i-200μL, futhi kunconyiwe IVolumu yokuqalwa yi-80μL.
Inketho 2.
I-QAAMP Viral RNA MINI KIT (52904) ekhiqizwa yi-Qiagen noma i-nucleic acid extraction noma i-jpp35-R) ekhiqizwa yiTiangen Biotech (Ltd.
