Izinhlobo Ezi-4 Zamagciwane Okuphefumula

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola ikhwalithi ye-2019-nCoV, igciwane le-influenza A, igciwane le-influenza B kanye negciwane le-respiratory syncytial virus i-nucleic acidskumuntuoamasampula e-ropharyngeal swab.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

I-HWTS-RT099- Izinhlobo Ezi-4 Zamagciwane Okuphefumula I-Nucleic Acid Detection Kit (Fluorescence PCR)-Uhlelo lwe-NED-ABI 7500 Real-Time PCR/ABI 7500 Izinhlelo ze-PCR Ezisheshayo Zesikhathi Sangempela/i-QuantStudio^®Izinhlelo ze-PCR zesikhathi sangempela ezi-5

I-HWTS-RT158-4 Izinhlobo Zamagciwane Okuphefumula I-Nucleic Acid Detection Kit (i-Fluorescence PCR)-内参Quasar 705

I-Epidemiology

Isifo Segciwane Le-Corona sika-2019, esibizwa ngokuthi "i-COVID-19", sibhekisela e-pneumonia ebangelwa yi-2019-nCoVukutheleleka.2019-nCoVigciwane le-coronavirus elivela kuhlobo lwe-β. I-COVID-19 iyisifo esithathelwanayo esibangelwa ukuphefumula okubukhali, futhi abantu bavame ukungenwa kalula. Njengamanje, umthombo wokutheleleka ikakhulukazi iziguli ezitheleleke yi-2019-nCoV, futhi abantu abanegciwane abangenazo izimpawu bangaba umthombo wokutheleleka. Ngokusekelwe ocwaningweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku eziyi-1-14, ikakhulukazi izinsuku ezi-3-7. Umkhuhlane, ukukhwehlela okomile kanye nokukhathala yizona zimpawu eziyinhloko. Iziguli ezimbalwa zazinezimpawus ezifanaukuminyana kwamakhala, ikhala eligelezayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo, njll..

Isiteshi

I-FAM	2019-nI-CoV
I-VIC(HEX)	I-RSV
CY5	I-IFV A
I-ROX	I-IFV B
I-NED	Ukulawula Kwangaphakathi

Amapharamitha Obuchwepheshe

Isitoreji	-18℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-9
Uhlobo Lwesifanekiso	I-Oropharyngeal swab
Ct	≤38
I-LoD	2019-nCoV: Amakhophi angu-300/mLIgciwane le-Influenza A/Igciwane le-Influenza B/Igciwane le-Respiratory syncytial virus: 500 Amakhophi/mL
Ukucaciswa	a) Imiphumela yokusabela okuxubile ikhombisa ukuthi akukho ukusabela okuxubile phakathi kwekhithi kanye ne-human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, i-parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci kanye ne-cryptococcus esanda kuzalwa kanye ne-human genomic nucleic acid. b) Amandla okulwa nokuphazamiseka: i-mucin ekhethiwe (60mg/mL), i-10% (v/v) yegazi kanye ne-phenylephrine (2mg/mL), i-oxymetazoline (2mg/mL), i-sodium chloride (kufaka phakathi izilondolozi) (20 mg/mL), i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-flunisolide (20μg/mL), i-triamcinolone acetonide (2mg/mL), i-budesonide (2mg/mL), i-mometasone (2mg/mL), i-fluticasone (2mg/mL), i-histamine hydrochloride (5mg/mL), i-alpha interferon (800IU/mL), i-zanamivir (20mg/mL), i-ribavirin (10mg/mL), i-oseltamivir (60ng/mL), i-peramivir (1mg/mL), i-lopinavir (500mg/mL), i-ritonavir(60mg/mL), i-mupirocin (20mg/mL), i-azithromycin (1mg/mL), i-ceftriaxone (40μg/mL), i-meropenem (200mg/mL), i-levofloxacin (10μg/mL) kanye ne-tobramycin (0.6mg/mL) ukuze kuhlolwe ukuphazamiseka, futhi imiphumela ikhombisa ukuthi izinto eziphazamisayo ezinamazinga okukhulunywe ngawo ngenhla azinawo ukusabela kokuphazamiseka emiphumeleni yokuhlolwa kwamagciwane.
Izinsimbi Ezisebenzayo	Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-TimeIzinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela

Ukugeleza Komsebenzi

Inketho 1.
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) ekhiqizwe yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Umthamo wesampula okhishwe ngu-200μL, kanti umthamo wokulungisa onconywayo ngu-80μL.
Inketho 2.
I-QIAamp Viral RNA Mini Kit (52904) ekhiqizwe yi-QIAGEN noma i-Nucleic Acid Extraction noma i-Purification Kit (YDP315-R) ekhiqizwe yi-Tiangen Biotech (Beijing) Co., Ltd. Umthamo wesampula okhishwe ngu-140μL, kanti umthamo wokuhlunga onconywayo ngu-60μL.

Okwedlule: Izinhlobo eziyi-12 ze-Respiratory Pathogen

Olandelayo: I-Adenovirus Universal

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