4 Izinhlobo Zamagciwane Okuphefumula
Igama lomkhiqizo
HWTS-RT099- 4 Izinhlobo Zamagciwane Okuphefumula I-Nucleic Acid Detection Kit (Fluorescence PCR)-内参是NED-ABI 7500 Real-Time PCR System/ABI 7500 Fast Real-Time PCR Systems/QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela
I-HWTS-RT158-4 Izinhlobo Zamagciwane Okuphefumula I-Nucleic Acid Detection Kit (Fluorescence PCR)-内参Quasar 705
I-Epidemiology
I-Corona Virus Disease 2019, ebizwa ngokuthi "i-COVID-19", isho inyumoniya ebangelwa2019-nCoVukutheleleka.2019-nCoViyi-coronavirus yohlobo lwe-β. I-COVID-19 yisifo esithathelwanayo esinamandla sokuphefumula, futhi inani labantu ngokuvamile liyathinteka. Njengamanje, umthombo wokutheleleka ikakhulukazi iziguli ezitheleleke yi2019-nCoV, kanye nabantu abangenazimpawu zesifo bangase babe umthombo wokutheleleka. Ngokusekelwe ophenyweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku ezingu-1-14, ikakhulukazi izinsuku ezingu-3-7. Umkhuhlane, ukukhwehlela okomile nokukhathala yizimpawu eziyinhloko. Iziguli ezimbalwa zazinezimpawus njengeukucinana kwamakhala, ikhala eligelezayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo, njll.
Isiteshi
| FAM | 2019-nI-CoV |
| I-VIC(HEX) | I-RSV |
| CY5 | IFV A |
| I-ROX | IFV B |
| I-NED | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | -18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | I-Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Copies/mLIgciwane lomkhuhlane A/Igciwane loMkhuhlane B/Igciwane le-Respiratory syncytial: 500Copies/mL |
| Ukucaciswa | a) Imiphumela ye-cross-reactivity ibonisa ukuthi akukho ukusabela okuphambene phakathi kwekhithi kanye ne-coronavirus yabantu i-SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, humanovirus metdiaoch, C. A, B, C, D, human pulmonary virus, epstein-barr virus, virus yesimungumungwane, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptocuscgenecoccus streptococcus, p. pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci kanye ne-cryptococcus esanda kuzalwa kanye ne-nucleic acid yomuntu. b) Ikhono lokunqanda ukugxambukela: khetha i-mucin (60mg/mL), 10% (v/v) yegazi ne-phenylephrine (2mg/mL), i-oxymetazoline (2mg/mL), i-sodium chloride (kuhlanganise nezivimbelakuvunda) (20 mg/mL), i-beclomethasone (20mg/msomL), i-dexamethasone (0) (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), i-fluticasone (2mg/mL), i-histamine hydrochloride (5mg/mL), i-alpha interferon (800IU/mL), i-zanamivirimvirvir (10mg rimg), i-zanamivirimvir/10mg rimg, i-alpha interferon (800IU/mL), i-zanamivirimvir/10mg rimg (60ng/mL), i-peramivir (1mg/mL), i-lopinavir (500mg/mL), i-ritonavir (60mg/mL), i-mupirocin (20mg/mL), i-azithromycin (1mg/mL), i-ceftriaxone (40μg/mL), i-meropenem (200mg/mL), i-levofloxamy (10mg/mL), i-levofloxamy (0.6mg/mL) yokuhlolwa kokuphazanyiswa, futhi imiphumela ibonisa ukuthi izinto eziphazamisayo ezinokugxila okukhulunywe ngazo ngenhla azinakho ukusabela kokuphazamiseka emiphumeleni yokuhlolwa yamagciwane. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR SystemApplied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems |
Ukugeleza komsebenzi
Inketho 1.
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) ekhiqizwe i-Jiangsu Macro & Micro-Test Coed. umthamo we-elution onconywayo ngu-80μL.
Inketho yesi-2.
I-QIAamp Viral RNA Mini Kit (52904) ekhiqizwe i-QIAGEN noma i-Nucleic Acid Extraction noma i-Purification Kit (YDP315-R) ekhiqizwe yi-Tiangen Biotech (Beijing) Co., Ltd. Isampula yevolumu ekhishiwe ingu-140μL, futhi ivolumu enconyiwe ye-elution ngu-60μL.
