Imithi Yokuphepha Ye-Aspirin
Igama lomkhiqizo
Ikhithi Yokuthola Imithi Yokuphepha ye-HWTS-MG050-Aspirin (i-Fluorescence PCR)
I-Epidemiology
I-aspirin, njengomuthi osebenzayo wokulwa nokuhlanganiswa kwama-platelet, isetshenziswa kabanzi ekuvimbeleni nasekwelapheni izifo zenhliziyo nemithambo yegazi. Ucwaningo luthole ukuthi ezinye iziguli zitholakale zingakwazi ukuvimbela ngempumelelo umsebenzi wama-platelet naphezu kokusetshenziswa kwe-aspirin isikhathi eside ngesilinganiso esiphansi, okungukuthi, ukumelana ne-aspirin (AR). Izinga licishe libe ngu-50%-60%, futhi kukhona umehluko osobala wobuhlanga. I-Glycoprotein IIb/IIIa (GPI IIb/IIIa) idlala indima ebalulekile ekuhlanganisweni kwama-platelet kanye ne-thrombosis ebukhali ezindaweni zokulimala kwemithambo yegazi. Izifundo zibonise ukuthi ama-polymorphisms ezakhi zofuzo adlala indima ebalulekile ekumelaneni ne-aspirin, ikakhulukazi egxile kuma-polymorphisms ezakhi zofuzo ze-GPIIIa P1A1/A2, PEAR1 kanye ne-PTGS1. I-GPIIIa P1A2 iyizakhi zofuzo eziyinhloko zokumelana ne-aspirin. Ukuguquka kwalesi sakhi sofuzo kushintsha isakhiwo sama-receptor e-GPIIb/IIIa, okuholela ekuxhumaneni phakathi kwama-platelet kanye nokuhlanganiswa kwama-platelet. Ucwaningo luthole ukuthi ukuvama kwama-allele e-P1A2 ezigulini ezingamelani ne-aspirin kwakuphakeme kakhulu kunasezigulini ezizwela i-aspirin, kanti iziguli ezine-homozygous mutations ye-P1A2/A2 zazingasebenzi kahle ngemva kokuthatha i-aspirin. Iziguli ezine-alleles ye-P1A2 eguquliwe ezithola i-stenting zinezinga le-subacute thrombotic event eliphindwe kahlanu kuneziguli ze-homozygous wild-type ze-P1A1, ezidinga imithamo ephezulu ye-aspirin ukuze kufezwe imiphumela yokulwa nokugayeka kwegazi. I-allele ye-PEAR1 GG isabela kahle ku-aspirin, kanti iziguli ezine-genotype ye-AA noma ye-AG ezithatha i-aspirin (noma zihlanganiswe ne-clopidogrel) ngemva kokufakelwa kwe-stent zine-myocardial infarction ephezulu kanye nokufa. I-genotype ye-PTGS1 GG inengozi ephezulu yokumelana ne-aspirin (HR: 10) kanye nezinga eliphezulu lezehlakalo zenhliziyo (HR: 2.55). I-genotype ye-AG inengozi ephakathi, futhi kufanele kuqashelwe kakhulu umphumela wokwelashwa kwe-aspirin. I-genotype ye-AA izwela kakhulu ku-aspirin, futhi izehlakalo zezehlakalo zenhliziyo ziphansi kakhulu. Imiphumela yokuthola yalo mkhiqizo imelela kuphela imiphumela yokuthola izakhi zofuzo ze-PEAR1, PTGS1, kanye ne-GPIIIa zabantu.
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-swab yomphimbo |
| CV | ≤5.0% |
| I-LoD | 1.0ng/μL |
| Izinsimbi Ezisebenzayo | Kusebenza ku-reagent yokuthola uhlobo I: Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR. Kusebenza ku-reagent yokuthola uhlobo lwesibili: I-EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza Komsebenzi
I-Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampula okhishwe ungama-200μL kanti umthamo wokulungisa onconywayo ungama-100μL.
