I-Aspirin Safety Medicine
Igama lomkhiqizo
I-HWTS-MG050-Aspirin Safety Medication Kit(Fluorescence PCR)
I-Epidemiology
I-Aspirin, njengesidakamizwa esisebenzayo sokuhlanganisa ama-platelet, isetshenziswa kabanzi ekuvimbeleni nasekwelapheni izifo zenhliziyo nemithambo yegazi.Ucwaningo luthola ukuthi ezinye iziguli zitholakale zingakwazi ukuvimbela ngempumelelo umsebenzi wamaplatelet naphezu kokusetshenziswa kwe-aspirin yedosi ephansi yesikhathi eside, okungukuthi, ukumelana ne-aspirin (AR). Izinga licishe libe ngu-50% -60%, futhi kunomehluko osobala wezinhlanga. I-Glycoprotein IIb/IIIa (GPI IIb/IIIa) idlala indima ebalulekile ekuhlanganisweni kweplatelet kanye ne-acute thrombosis ezindaweni zokulimala kwemithambo. Ucwaningo luye lwabonisa ukuthi ama-polymorphisms ofuzo adlala indima ebalulekile ekuphikisweni kwe-aspirin, ikakhulukazi egxile ku-GPIIIa P1A1/A2, PEAR1 kanye ne-PTGS1 gene polymorphisms. I-GPIIa P1A2 iwufuzo oluyinhloko lokumelana ne-aspirin. Uguquko kulesi sakhi sofuzo lushintsha ukwakheka kwama-receptors e-GPIIb/IIIa, okuholela ekuxhumekeni phakathi kwama-platelet nokuhlangana kwe-platelet. Ucwaningo lwathola ukuthi imvamisa ye-P1A2 alleles ezigulini ezimelana ne-aspirin yayiphezulu kakhulu kunaleyo ezigulini ezizwela i-aspirin, futhi iziguli ezinezinguquko ze-homozygous ze-P1A2/A2 zazingasebenzi kahle ngemva kokuthatha i-aspirin. Iziguli ezine-mutant P1A2 alleles ezibhekene ne-stenting zinezinga lomcimbi we-subacute thrombotic eliphindwe kahlanu kune-P1A1 yeziguli zohlobo lwasendle oluyi-homozygous, oludinga imithamo ephezulu ye-aspirin ukuze kuzuzwe imiphumela ye-anticoagulant. I-PEAR1 GG allele isabela kahle ku-aspirin, futhi iziguli ezine-AA noma i-AG genotype ezithatha i-aspirin (noma ehlanganiswe ne-clopidogrel) ngemva kokufakwa kwe-stent ziba ne-infarction ephezulu ye-myocardial kanye nokufa. I-PTGS1 GG genotype inengozi enkulu yokumelana ne-aspirin (HR: 10) kanye nezigameko eziphezulu zezehlakalo zenhliziyo nemithambo yegazi (HR: 2.55). I-genotype ye-AG inobungozi obumaphakathi, futhi kufanele kuqashelwe kakhulu umphumela wokwelashwa kwe-aspirin. I-AA genotype izwela kakhulu i-aspirin, futhi izehlakalo zezehlakalo zenhliziyo nemithambo yegazi ziphansi uma kuqhathaniswa. Imiphumela yokutholwa yalo mkhiqizo imelela kuphela imiphumela yokutholwa yezakhi zofuzo zabantu ze-PEAR1, PTGS1, ne-GPIIa.
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-swab yomphimbo |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Izinsimbi Ezisebenzayo | Isebenza ekuthayipheni i-reagent yokuthola i-I: I-Applied Biosystems 7500 Real-Time PCR Systems, I-QuantStudio®5 Amasistimu e-PCR wesikhathi sangempela, I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), I-LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), I-BioRad CFX96 Real-Time PCR System, I-BioRad CFX Opus 96 Real-Time PCR System. Kusebenza kuhlobo lwe-II esebenzayo yokuthola: EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza komsebenzi
I-Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nge-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ivolumu yesampula ekhishiwe ingu-200μL futhi ivolumu ye-lution enconyiwe ingu-100μL.
