I-Candida Albicans Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-FG001A-Candida Albicans Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Uhlobo lwe-Candida luyisitshalo esikhulu kunazo zonke esivamile se-fungus emzimbeni womuntu. Lutholakala kabanzi endleleni yokuphefumula, endleleni yokugaya ukudla, endleleni yokungena korogenital nakwezinye izitho ezixhumana nomhlaba wangaphandle. Ngokuvamile, alunazo izifo futhi lungolwamabhaktheriya abangela izifo angosomathuba. Ngenxa yokusetshenziswa kabanzi kwe-immunosuppressant kanye nenani elikhulu lama-antibiotic e-broad-spectrum, kanye ne-tumor radiotherapy, i-chemotherapy, ukwelashwa okuhlaselayo, ukufakelwa kwezitho, isitshalo esivamile asilingani futhi ukutheleleka kwe-candida kwenzeka endleleni yokungena korogenital kanye nendlela yokuphefumula.
Ukutheleleka nge-Candida komgudu wokuzala kungenza abesifazane bahlushwe yi-Candida vulva kanye ne-vaginitis, okuthinta kakhulu impilo yabo nomsebenzi wabo. Ukwanda kwe-candidiasis yomgudu wokuzala kuyanda unyaka nonyaka, phakathi kwawo ukutheleleka nge-Candida komgudu wokuzala wesifazane kubalelwa cishe ku-36%, kanti ukutheleleka nge-Candida komgudu wokuzala wesilisa kubalelwa cishe ku-9%, phakathi kwawo, i-Candida albicans (CA) yiyo ebangela ukutheleleka kakhulu, kubalelwa cishe ku-80%. Ukutheleleka nge-fungus, ngokuvamile i-Candida albicans, kuyimbangela ebalulekile yokufa esibhedlela, kanti ukutheleleka nge-CA kubalelwa cishe ku-40% weziguli zase-ICU. Phakathi kwazo zonke izifo ze-visceral fungal, ukutheleleka nge-fluor fungal yikona okuvame kakhulu, futhi lo mkhuba uyanda unyaka nonyaka. Ukuxilongwa kusenesikhathi kanye nokuhlonzwa kokutheleleka kwe-fluor fungal kubaluleke kakhulu emtholampilo.
Isiteshi
| I-FAM | Ama-Candida Albicans |
| I-VIC/HEX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Ukuphuma koketshezi esithweni sangasese sowesifazane, isikhwehlela |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | 1 × 103Amakhophi/mL |
| Ukucaciswa | Akukho ukuhlangana kwezifo nezinye izifo ezibangelwa ukutheleleka kwe-genitourinary tract ezifana ne-Candida tropicalis, i-Candida glabrata, i-Trichomonas vaginalis, i-Chlamydia trachomatis, i-Ureaplasma urealyticum, i-Neisseria gonorrhoeae, i-Group B Streptococcus, i-herpes simplex virus type 2 kanye nezinye izifo ezibangelwa ukutheleleka kwe-pathogen yokuphefumula ezifana ne-adenovirus, i-Mycobacterium tuberculosis, i-Klebsiella pneumoniae, i-measles virus kanye namasampula avamile e-sputum yabantu. |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
Ama-reagent okukhipha anconyiwe: I-Macro & Micro-Test Sample Release Release Release Reagent (HWTS-3005-8)
Inketho 2.
Ama-reagent okukhipha anconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006)
