I-Candida Albicans Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-FG005-Nucleic Acid esekelwe ku-Enzymatic Probe Isothermal Amplification (EPIA) yama-Candida Albicans
Isitifiketi
CE
I-Epidemiology
Uhlobo lwe-Candida luyisitshalo esikhulu kunazo zonke esivamile se-fungus emzimbeni womuntu, esitholakala kabanzi endleleni yokuphefumula, endleleni yokugaya ukudla, endleleni yokungena kanye nezinye izitho ezixhumana nomhlaba wangaphandle. Alunazo izifo ngokuvamile futhi lungolwamabhaktheriya abangela izifo angenawo amagciwane. Ngenxa yokusetshenziswa okukhulu kwama-immunosuppressive agents, ukuthuthukiswa kwe-tumor radiotherapy, i-chemotherapy, ukwelashwa okungena emzimbeni, kanye nokufakelwa kwezitho, kanye nokusetshenziswa kabanzi kwenani elikhulu lama-antibiotic e-broad-spectrum, isitshalo esivamile siyaba nokungalingani, okuholela ekuthelelekeni kwe-Candida endleleni yokungena kanye nendlela yokuphefumula.
Ukutheleleka nge-Candida emgudwini we-genitourinary kungabangela abesifazane ukuthi bahlushwe yi-candidal vulvitis kanye ne-vaginitis, futhi kubangele amadoda ukuthi ahlushwe yi-candidal balanitis, i-acroposthitis kanye ne-prostatitis, okuthinta kakhulu impilo nomsebenzi weziguli. Izinga lokusabalala kwe-candidiasis emgudwini we-genital liyakhula unyaka nonyaka. Phakathi kwazo, ukutheleleka kwe-candida emgudwini we-genital tract kwabesifazane kubalelwa cishe ku-36%, kanti abesilisa babalelwa cishe ku-9%, kanti ukutheleleka kwe-Candida albicans (CA) yikona okuyinhloko, okubalelwa cishe ku-80%.
Ukutheleleka ngesikhunta okujwayelekile kokutheleleka nge-Candida albicans kuyimbangela ebalulekile yokufa ngenxa yokutheleleka kwe-nosocomial. Phakathi kweziguli ezibucayi ezise-ICU, ukutheleleka nge-Candida albicans kubalelwa cishe ku-40%. Phakathi kwazo zonke izifo zesikhunta ze-visceral, ukutheleleka ngesikhunta se-pulmonary yikona okuvame kakhulu futhi kuyanda unyaka nonyaka. Ukuxilongwa kusenesikhathi kanye nokuhlonzwa kokutheleleka ngesikhunta se-pulmonary kubaluleke kakhulu emtholampilo.
Imibiko yamanje yezokwelapha yezinhlobo ze-Candida albicans ifaka phakathi uhlobo A, uhlobo B, kanye nohlobo C, futhi izinhlobo ezintathu ze-genotypes zingaphezu kuka-90%. Ukuxilongwa okunembile kokutheleleka kwe-Candida albicans kunganikeza ubufakazi bokuxilongwa nokwelashwa kwe-candidal vulvitis kanye ne-vaginitis, i-candidal balanitis yabesilisa, i-acroposthitis kanye ne-prostatitis, kanye nokutheleleka kwe-Candida albicans yomgudu wokuphefumula.
Isiteshi
| I-FAM | i-CA nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ Ebumnyameni; I-Lyophilized: ≤30℃ Ebumnyameni |
| Isikhathi sokuphelelwa yisikhathi | Uketshezi: izinyanga ezingu-9; I-Lyophilized: izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-Swab Yomgudu Wezitho Zangasese, I-Sputum |
| Tt | ≤28 |
| CV | ≤10.0% |
| I-LoD | Amakhophi angu-5/µL, amabhaktheriya angu-102/mL |
| Ukucaciswa | Akukho ukuhlangana kwezifo nezinye izifo ezibangelwa ukutheleleka kwe-genitourinary tract, njenge-Candida tropicalis, i-Candida glabrata, i-Trichomonas vaginalis, i-Chlamydia trachomatis, i-Ureaplasma urealyticum, i-Neisseria gonorrhoeae, i-Group B streptococcus, i-Herpes simplex virus type 2, njll.; akukho ukuhlangana kwezifo phakathi kwale kit nezinye izifo ezibangelwa ukutheleleka kokuphefumula, njenge-Adenovirus, i-Mycobacterium tuberculosis, i-Klebsiella pneumoniae, i-Measles, i-Candida tropicalis, i-Candida glabrata kanye namasampula ajwayelekile e-sputum yabantu, njll. |
| Izinsimbi Ezisebenzayo | Uhlelo Lokuthola Isothermal Lokukhanya Kwe-Easy Amp Real-time (HWTS1600) Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
