Ikhithi Yokuthola Ama-Antibody e-Chikungunya Virus IgM/IgG (igolide le-Colloidal)
Igama lomkhiqizo
Ikhithi Yokuthola Ama-Antibody e-Chikungunya Virus IgM/IgG (igolide le-Colloidal)
I-Epidemiology
I-Chikungunya yisifo esithathelwanayo esibangelwa yigciwane le-chikungunya (CHIKV), esidluliselwa omiyane be-Aedes, futhi sibonakala ngomkhuhlane, ukuqubuka kanye nobuhlungu bamalunga. I-Chikungunya yaqinisekiswa ukuthi ivame eTanzania ngo-1952, futhi igciwane latholakala ngo-1956. Lesi sifo sivame kakhulue-Afrika naseNingizimu-mpumalanga ye-Asia, futhi ibangele ubhubhane olukhulu esifundeni sase-Indian Ocean eminyakeni yamuva nje [1]. Igciwane le-chikungunya linohlobo lwe-oneerotype futhi lingahlukaniswa ngezinhlobo ezintathu zofuzo, okungukuthi uhlobo lwaseNtshonalanga Afrika, uhlobo lwaseCentral-East-Southern Africa kanye nohlobo lwase-Asia [2]. Igciwane le-chikungunya selitholakale emazweni angaphezu kuka-110 e-Asia, e-Afrika, eYurophu naseMelika [3]. Izimpawu zesifo zifana nezomkhuhlane we-dengue, futhi kulula ukuzithola kabi. Nakuba izinga lokufa liphansi kakhulu, kulula ukwakha ukuqubuka okukhulu kanye nobhubhane ezindaweni ezinobuningi obukhulu bomiyane [4]. Ama-antibodies e-IgM kanye ne-IgG egazini lomuntu lonke, i-serum, noma i-plasma, futhi angasetshenziswa njengethuluzi lokuxilonga elisizayo lokutheleleka kwegciwane le-chikungunya ekuqaleni emtholampilo. Leli khithi lifanele ukuthola ama-antibodies e-Chikungunya kubantu ababonisa izimpawu ezithile.
Amapharamitha Obuchwepheshe
| Isitoreji | 4~30℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingama-24 |
| Uhlobo Lwesifanekiso | Amasampula e-serum, i-plasma, kanye negazi eliphelele |
| I-LoD | Ukutholwa kwe-IgM: ukuzwela okungu-97.56%, ukucaciswa98.74%, izinga lokuhlangana okuphelele 98.50%. Ukutholwa kwe-IgG: ukuzwela okungu-97.67%, ukucaciswa okungu-98.73%, izinga lokuhlangana okuphelele okungu-98.50%. |
| Ukucaciswa | Akukho ukusabela okuhlanganisiwe okubonwe ekuhlolweni kokusabela okuhlanganisiwe kwale khithi eneIgciwane le-encephalitis laseJapan, igciwane le-encephalitis elithwalwa yizikhaza, umkhuhlane omkhulu one igciwane le-thrombocytopenia syndrome, igciwane le-Xinjiang hemorrhagic fever, I-Hantavirus, igciwane le-Dengue, igciwane le-Hepatitis C, igciwane le-Influenza A kanye ne-Influenza Igciwane le-B. |
Ukugeleza komsebenzi:
Sicela ufunde incwadi yemiyalelo ngokucophelela ngaphambi kokuhlola. Ngaphambi kokuhlola, khipha wonke ama-reagent namasampula azohlolwa ezimweni zokugcina bese uwavumela ukuthi abuyele ekushiseni kwegumbi. Ukuhlolwa kufanele kwenziwe ekushiseni kwegumbi.
Amasampula e-serum, i-plasma, kanye negazi eliphelele:
1. Khipha ikhadi lokuhlola esikhwameni se-aluminium foil bese ulibeka endaweni eyisicaba neyomile;
2. Sebenzisa i-pipette ukuze wengeze ithonsi elilodwa le-serum, i-plasma, noma igazi eliphelele (cishe i-10µL) emthonjeni wesampula "S";
3. Bese ufaka amaconsi amabili esampula exubile (cishe ama-70 µL) emthonjeni wesampula "S";
4. Yenza isinqumo somphumela zingakapheli imizuzu eyi-15-20. Umphumela oboniswe ngemva kwemizuzu engama-20 awusebenzi.
