Izinhlobo eziyisishiyagalombili zamagciwane okuphefumula
Igama lomkhiqizo
HWTS-RT184-Izinhlobo eziyisishiyagalombili zama-virus okuphefumula ze-nucleic acid detection detection (Fluorescence PCR)
I-Epidemiology
Ukutheleleka kwe-Respiratory Tract kuwuhlobo oluvame kakhulu lwesifo somuntu, olungenzeka kunoma yibuphi ubulili, ubudala kanye nesifunda, futhi kungenye yezimbangela ezibaluleke kakhulu zokuxineka nokufa emhlabeni[1]. Amagciwane ajwayelekile ajwayelekile afaka umkhuhlane igciwane (ifv a), umkhuhlane b virus (IFV B), i-adenovirus, i-rust metapneumovirus, i-mycoplasma pneumoniae, njll.[2,3]. Izimpawu zomtholampilo nezimpawu ezibangelwa ukutheleleka kwezifo zokuphefumula ziyefana, kepha ukutheleleka okubangelwa izindlela ezahlukahlukene zokwelashwa zinezindlela ezahlukahlukene zokwelashwa, imiphumela yekola kanye nenkambo yesifo[4,5]. Njengamanje, izindlela eziphambili zokutholwa kwelabhoratri lokutholwa kwamagciwane wokuphefumula kufaka phakathi: Ukuhlukaniswa kwegciwane, ukutholwa kwe-Antigen kanye nokutholwa kwe-nucleic acid. Le kit ibona futhi ikhombe ama-aucleic acid athile athile kubantu abanezimpawu nezimpawu zokutheleleka kokuphefumula, ngokubambisana neminye imiphumela yezomtholampilo nakwelabhoratri ukusiza ekutholakaleni ukutheleleka ngegciwane.
Amapharamitha Ezobuchwepheshe
| Ukubeka | 2-8 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | I-Oropharyngengeal Swab; Nasopharyngel swab |
| Ct | IFV A, IFVB, RSV, ADD, HMPV, RHV, PV, MP CT≤35 |
| CV | <5.0% |
| Indlu edayisa insimbi | Amakhophi angama-200 / ml |
| Ukucacisa | Ukuvuselelwa kabusha kwesiphambano: Akukho ukubheja okwenziwa yisiphambano phakathi kwe-kit ne-boca virus, i-cytomegalovirus, igciwane le-entoum-barl, i-antovirus, igciwane le-coronavirus, i-coronavirus yomuntu, i-potavirus, i-noronirus , Chlamydia pneumoniae, I-Streptococcus Pneumoniae, Klebsoccus Pneumoniae, LegionoCus, Pneumosporora, Haemophilus Umkhuhlane, i-Staphylococcus aureus, i-Mycobacterium Tuberculosis, i-Gonococcus, ama-Albicans, uCandida Glabra, I-Aspergillus Fumigatus, ama-cryptococcus neoformans, i-streptococcus salivarmarius, eMoraxella Catarrh, i-lactobacillus, i-corybacteracterium, i-DNA yomuntu. Ukuhlolwa kokuphazanyiswa I-20mg / ml), dexamethasone (20mg / ml), I-Fluniacetone (20μg / ml), i-Triamcinolone (2mg / ML), i-Budesonide (1mg / ML), i-MomeTasone (2mg / ml), i-fluticasone (2mg / ml), i-anzocaine (10% (10%), i-menl (10%), Zanamivir . I-Font Corchra |
| Izinsimbi ezisebenzayo | Kusebenza ku-Type I realent realent: Kusetshenzisiwe Biosystems 7500 Systems real-time PCR Systems, QuantStudio®Amasistimu we-PRCR yangempela ama-PRCR, SLAN-96P real-time PCRS Systems (Hongshi Technology CRRECH CCREMS Co, Ltd.) I-Cycler yesikhathi esezingeni eliphezulu (Suzhou Molarray Co, Ltd.), Biorad CFX96 Uhlelo lwe-PCR lwesikhathi sangempela, i-Biorad CFX Opus 96 Uhlelo lwe-PRCR lwesikhathi sangempela se-PRR. Kusebenza ku-Type II ukutholwa reagent: eudemonm ao800 (HWS-EQ007) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd. |
Ukugeleza komsebenzi
I-Macro & Micro-Test Viral Viral / RNA KIT (HWTS-3017) (engasetshenziswa nge-macro & micro-avin autory automactor ye-nucleic acid extractor (HWS-3006c, ne-HWS-3006B), kanye ne-Macro & Micro-Test DNA / I-RNA KIT (HWTS-3017-8) (engasetshenziswa nge-eudermonTM I-AIO800 (HWS-EQ007)) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd.
Ivolumu yesampula ekhishwe i-200μL kanye nevolumu yokuqedwa enconyiwe ingu-150μL.
