I-Enterovirus Universal, ev71 neCoxa16
Igama lomkhiqizo
I-HWTS-EV026B-ENYOVIRUS Universal, EV71 ne-COXA16 Kit Detection Dectional (Fluorescence PCR)
Hwts-ev020y / z-Free ENTERVOVIRUS Universal, EV71 ne-COXA16 Kit Dectional Dection CCRICS (Fluescence PCR)
Isitifiketi
Ce / MDA (HWTS-EV026)
I-Epidemiology
Isifo sesandla sesandla (HFMD) yisifo esivamile esithelelekayo ezinganeni. Kwenzeka kakhulu ezinganeni ezingaphansi kweminyaka engu-5, futhi kungadala ama-herpes ezandleni, izinyawo, umlomo kanye nezinye izingxenye, futhi inani elincane lezingane lingadala izinkinga ezinjenge-myocarditis, njll. I-meningincephalititis, njll. Izifo ziwohloka ngokushesha futhi zithambekele ekufeni uma zingalashwa ngokushesha.
Njengamanje, ama-serotypes angama-108 atholakele, ahlukaniswe ngamaqembu amane: a, B, C no-D. ENTENTOVIRUSES abadala abahlukahlukene, kepha i-ENTOVIRUS 71 (ev71) kanye neCoxsaIVirus A16 (ev716) ajwayelekile kakhulu futhi Isengezo ku-HFMD, kungadala izinkinga zesistimu emikhulu emikhulu njenge-meningitis, encephalitis, kanye ne-acute ukukhubazeka kwe-flaccid.
Insungubezi
| -Yenza | I-entovirus |
| I-VIC (Hex) | Coxa16 |
| Ivupho | Ev71 |
| Cy5 | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | Uketshezi: ≤-18 ℃ ebumnyameniI-Lyophilization: ≤30 ℃ |
| Ishalofu-impilo | Uketshezi: izinyanga eziyi-9I-Lyophilization: Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | Isampula ye-throat swab, uketshezi lwe-herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| Indlu edayisa insimbi | 500copies / ml |
| Izinsimbi ezisebenzayo | Ingaqondanisa amathuluzi we-PCR e-PCR eseMakethe emakethe.I-ABI 7500 real-time PCRS Systems I-ABI 7500 Fast Real-Time Pcr Systems SLAN-96P real-time PCRS Systems I-QuantStudio®5 Systems real-time PCREMS I-Lightcycler®480 Izinhlelo ze-PCR zangempela zesikhathi sangempela I-LineGene 9600 Plus Real-Time Pcc Retection Systems I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Isixazululo se-PCR esiphelele
I-realent enconyiwe I-realent: Macro & Micro-Vival DNA / RNA KIT (HWTS-3017) (angasetshenziswa nge-macro & micro-avin autory extractor (HWS-EQ011)) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd. Ukukhishwa kufanele kwenziwe ngokuya ngencwadi yokufundisa. Ivolumu yesampula ekhishwe i-200μL, futhi ivolumu yokuqedwa enconyiwe ingu-80μL.
I-reagent enconyelwe i-reagent: I-Macro & Micro-Test Sampula Sampula Represe Reagent (HWS-3005-8). Ukukhishwa kufanele kwenziwe ngokuya ngencwadi yokufundisa. Amasampula okukhishwa yi-Oropharyngell Swabs noma ama-herpes fluid amasampula eziguli eziqoqwe esizeni. Faka ama-swabs aqoqiwe ngqo kwi-macro & micro-test Saint Reled Reled Recent, Vortex bese uxuba kahle, indawo yokushisa yegumbi imizuzu engu-5, uthathe bese uxuba uhlanganise kahle ukuthola i-rna yesampula ngayinye.
I-realent enconyiwe ye-realent: Qiaamp Viral RNA MINI KIT (52904) nge-Qiagen noma nge-nucleic acid extraction noma i-reagent yokuhlanza (ypp15-r). Ukukhishwa kufanele kwenziwe ngokuqinile nge-Instruction Manual.
