I-Enterovirus Universal, EV71 kanye ne-CoxA16
Igama Lomkhiqizo
I-HWTS-EV026B-Enterovirus Universal, EV71 kanye ne-CoxA16 Nucleic Acid Detection Kit(Fluorescence PCR)
I-HWTS-EV020Y/Z-I-Enterovirus Universal, EV71 ne-CoxA16 Nucleic Acid Detection Kit(Fluorescence PCR)
Isitifiketi
CE/MDA (HWTS-EV026)
I-Epidemiology
I-Hand-foot-mouth disease (HFMD) yisifo esithathelwanayo esivamile ezinganeni. Ngokuvamile kwenzeka ezinganeni ezingaphansi kweminyaka engu-5 ubudala, futhi kungabangela i-herpes ezandleni, ezinyaweni, emlonyeni nakwezinye izingxenye, futhi inani elincane lezingane lingabangela izinkinga ezifana ne-myocarditis, i-pulmonary edema, i-aseptic meningoencephalitis, njll. Izingane ezigula kakhulu ziwohloka ngokushesha futhi zivame ukufa uma zingelashwa ngokushesha.
Njengamanje, kutholakale ama-serotypes angu-108 we-enteroviruses, ahlukaniswe ngamaqembu amane: A, B, C kanye no-D. Ama-Enteroviruses abangela i-HFMD ahlukahlukene, kodwa i-enterovirus 71 (EV71) ne-coxsackievirus A16 (CoxA16) yizona ezivame kakhulu futhi ngaphezu kwe-HFMD, zingabangela i-encephalitis enzima efana ne-encephalitis, i-paralysis eyinkimbinkimbi, i-paralysis system.
Isiteshi
| FAM | I-Enterovirus |
| I-VIC (HEX) | I-CoxA16 |
| I-ROX | EV71 |
| CY5 | Ukulawula kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ ebumnyameniI-Lyophilization: ≤30℃ |
| Impilo yeshelufu | Uketshezi: izinyanga ezingu-9I-Lyophilization: izinyanga eziyi-12 |
| Uhlobo Lwesifanekiso | Isampula ye-swab yomphimbo, i-Herpes fluid |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 500Amakhophi/mL |
| Izinsimbi Ezisebenzayo | Ingakwazi ukufanisa amathuluzi e-PCR e-fluorescent avamile emakethe.I-ABI 7500 Real-Time PCR Systems ABI 7500 Fast Real-Time PCR Systems SLAN-96P Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems I-LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Isixazululo se-PCR esiphelele
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ivolumu yesampula ekhishiwe ingu-200μL, futhi ivolumu ye-lution enconyiwe ingu-80μL.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Ukukhishwa kufanele kwenziwe ngokulandela imanuwali yeziqondiso. Amasampula okukhishwa ama-oropharyngeal swabs noma amasampula e-herpes fluid eziguli aqoqwe esizeni. Engeza ama-swabs aqoqiwe ngqo ku-Macro & Micro-Test Sample Release Reagent, i-vortex bese uxuba kahle, ubeke endaweni efudumele imizuzu emi-5, ukhiphe bese uyaphendulela futhi uhlanganise kahle ukuze uthole i-RNA yesampula ngayinye.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-QIAamp Viral RNA Mini Kit (52904) nge-QIAGEN noma i-Nucleic Acid Extraction noma i-Purification Reagent (YDP315-R). Ukukhishwa kufanele kwenziwe ngokuhambisana ngokuqinile nencwadi yeziqondiso.
