Igciwane lehlathi elithi encephalitis
Igama lomkhiqizo
I-HWTS-FE006 Forest Encephalitis Virus NUCLEIC Acid Detection Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Forest Encephalitis (FE), eyaziwa nangokuthi i-endencephalititis ebhulwayo (i-TICKET-Bornence encephalitis, i-TBE), iyisifo esithelelekayo sohlelo oluphakathi lwezinzwa olubangelwa yigciwane lehlathi elibangelwa yigciwane lezinzwa. Igciwane le -ntuct--encephalitis lingelakahlobo lwe-genus flavivivirus yomndeni we-flaviviridae. Izinhlayiya zegciwane ziyindilinga nge-40-50nm. Isisindo samangqamuzana singaba ngu-4 × 106I-DA, futhi leli gciwane genome liwumqondo omuhle, i-rna ekhubazekile eyodwa[1]. Ngokwengqondo, kubonakaliswa ngumkhuhlane ophakeme, ikhanda, i-coma, ukuqala okusheshayo kokucasuka kwe-veningeeal, kanye nokukhubazeka kwemisipha kwentamo nezingalo, futhi kunesilinganiso sokufa okuphezulu. Ukuxilongwa kusenesikhathi nokushesha kwe-Forest Encephalitis Virus kuyisihluthulelo sokwelashwa kwehlathi elithi Encephalitis, kanye nokusungulwa kwendlela elula, ethize futhi esheshayo futhi esheshayo futhi esheshayo futhi ngokushesha indlela yokuxilongwa kwendlela yokuxilongwa kubaluleke kakhulu ekuhlolweni komtholampilo kwe-Incephalitis[1,2].
Insungubezi
| -Yenza | I-PRETH Encephalitis Virus nucleic acid |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-9 |
| Uhlobo lwe-Specimen | i-serum entsha |
| Tt | ≤38 |
| CV | ≤5.0% |
| Indlu edayisa insimbi | 500copies / ml |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection Systems I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
I-reaglent enconyiwe ye-realent: ukukhishwa kwe-nucleic acid noma i-punication kit (yppp15-R) nguTiangen Biotech (Beijing) Co, Ltd., Ukukhishwa kufanele kwenziwe ngokwemiyalo ngokuqinile. Ivolumu yesampula ekhishwe inconyelwe yi-140μL futhi ivolumu yokuqedwa enconyiwe ingu-60μL.
I-realent enconyiwe: I-Macro & Micro-Test General DNA / RNA KIT (HWTS-3017-50, HWS-3017-50, HWS-3017-48, i-HWS-3017-48, ne-Macro & Micro-Test Aptoutic acid extractor ( I-HWTS-3006, HWTS-3006C, HWTS-3006B). Ukukhishwa kufanele kwenziwe ngokuya ngemiyalo ngokuqinile. Ivolumu yesampula ekhishwe inconyelwe ingu-200μL futhi ivolumu yokuqedwa enconyiwe liyi-80μL.
