I-Forest Encephalitis Virus
Igama lomkhiqizo
I-HWTS-FE006 I-Forest Encephalitis Virus Nucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
I-Forest encephalitis (FE), eyaziwa nangokuthi i-encephalitis ethwala umkhaza (i-Tick-borne encephalitis, i-TBE), isifo esithathelwanayo esiyingozi sesimiso sezinzwa esiphakathi esibangelwa igciwane le-encephalitis yasehlathini. I-Forest encephalitis virus ingeyohlobo lwe-Flavivirus yomndeni wakwaFlaviviridae. Izinhlayiya zegciwane ziyindilinga ezinobubanzi obungu-40-50nm. Isisindo samangqamuzana singaba ngu-4 × 106I-Da, kanye ne-genome yegciwane i-RNA enomucu owodwa[1]. Ngokomtholampilo, kubonakala ngomkhuhlane ophakeme, ikhanda elibuhlungu, ukuquleka, ukucasuka kwe-meningeal ngokushesha, nokukhubazeka kwemisipha yentamo nezitho, futhi inezinga eliphezulu lokufa. Ukuxilongwa kusenesikhathi futhi ngokushesha kwegciwane le-encephalitis yehlathi kuyisihluthulelo sokwelashwa kwe-encephalitis yamahlathi, futhi ukusungulwa kwendlela elula, eqondile futhi esheshayo yokuxilongwa kwe-etiological kubaluleke kakhulu ekuxilongweni komtholampilo kwe-encephalitis yamahlathi.[1,2].
Isiteshi
| FAM | ihlathi encephalitis virus nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | i-serum entsha |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500Amakhophi/mL |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela LineGene 9600 Plus Real-Time PCR Detection Systems I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Nucleic Acid Extraction noma Ikhithi Yokuhlanza (YDP315-R) yi-Tiangen Biotech(Beijing) Co., Ltd., ukukhishwa kufanele kwenziwe ngokuvumelana neziyalezo ngokuqinile. Ivolumu yesampula ekhishiwe enconyiwe ingu-140μL futhi ivolumu ye-elution enconyiwe ingu-60μL.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-06060, HWTS-30, HWTS-3017-96) HWTS-3006B). ukukhishwa kufanele kwenziwe ngokulandela imiyalelo ngokuqinile. Ivolumu yesampula ekhishiwe enconyiwe ingu-200μL futhi ivolumu ye-elution enconyiwe ingu-80μL.
