Igciwane le-Forest Encephalitis
Igama lomkhiqizo
Ikhithi Yokuthola I-Nucleic Acid ye-HWTS-FE006 Forest Encephalitis Virus (i-Fluorescence PCR)
I-Epidemiology
I-Forest encephalitis (FE), eyaziwa nangokuthi i-tick-borne encephalitis (i-tick-borne encephalitis, TBE), iyisifo esithathelwanayo esibi sesimiso sezinzwa esiyinhloko esibangelwa igciwane le-forest encephalitis. Igciwane le-forest encephalitis lingelohlobo lwe-Flavivirus lomndeni wakwa-Flaviviridae. Izinhlayiya zegciwane ziyindilinga enobubanzi obungu-40-50nm. Isisindo sama-molecule singama-4×106I-Da, futhi i-genome yegciwane iyi-RNA enomqondo omuhle, enezintambo eyodwa[1]. Ngokwezokwelapha, ibonakala ngomkhuhlane ophakeme, ikhanda elibuhlungu, i-coma, ukuqala okusheshayo kokucasuka kwe-meningeal, kanye nokukhubazeka kwemisipha yentamo nemilenze, futhi inezinga eliphezulu lokufa. Ukuxilongwa kusenesikhathi nangokushesha kwegciwane le-forest encephalitis kuyisihluthulelo sokwelashwa kwe-forest encephalitis, futhi ukusungulwa kwendlela elula, eqondile nesheshayo yokuxilonga i-etiological kubaluleke kakhulu ekuxilongweni kwe-forest encephalitis emtholampilo.[1,2].
Isiteshi
| I-FAM | igciwane le-forest encephalitis nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | i-serum entsha |
| Tt | ≤38 |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-500/mL |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
I-reagent yokukhipha enconyiwe: I-Nucleic Acid Extraction noma i-Purification Kit (YDP315-R) yi-Tiangen Biotech(Beijing) Co.,Ltd., ukukhishwa kufanele kwenziwe ngokwemiyalelo eqinile. Ivolumu yesampula enconyiwe ekhishwe ingu-140μL kanti ivolumu yokususa enconyiwe ingu-60μL.
I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006, HWTS-3006C, HWTS-3006B). Ukukhipha kufanele kwenziwe ngokwemiyalelo eqinile. Ivolumu yesampula enconyiwe ingu-200μL kanti ivolumu yokususa enconyiwe ingu-80μL.
