I-Zaire eyomisiwe ngeqhwa kanye ne-Sudan Ebolavirus Nucleic Acid

Incazelo emfushane:

Le khithi ifaneleka ukuthola i-Ebolavirus nucleic acid kumasampula e-serum noma e-plasma ezigulini okusolwa ukuthi zinegciwane i-Zaire ebolavirus (EBOV-Z) kanye ne-Sudan ebolavirus (EBOV-S), ukuze kutholakale ukuthayipha.


Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

I-HWTS-FE035-Ikhithi Yokuthola I-Nucleic Acid Eyomisiwe e-Zaire nase-Sudan Ebolavirus (i-Fluorescence PCR)

I-Epidemiology

I-Ebolavirus ingeye-Filoviridae, okuyigciwane le-RNA elingenayo imigqa eyodwa elingakahlukaniswa. Ama-virus ayimicu emide enobude obumaphakathi be-virion obungu-1000nm kanye nobubanzi obungu-100nm. I-genome ye-Ebolavirus iyi-RNA engenayo imigqa engakahlukaniswa enobukhulu obungu-18.9kb, equkethe amaprotheni ayi-7 ahlelekile kanye neprotheni eyodwa engeyona eyakhekile. I-Ebolavirus ingahlukaniswa ngezinhlobo ezifana ne-Zaire, i-Sudan, i-Bundibugyo, i-Tai Forest kanye ne-Reston. Phakathi kwazo, uhlobo lwe-Zaire kanye nohlobo lwe-Sudan kubikwe ukuthi lubangela ukufa kwabantu abaningi ngenxa yokutheleleka. I-EHF (i-Ebola Hemorrhagic Fever) yisifo esithathelwanayo esibangelwa ukopha okubukhali esibangelwa yi-Ebolavirus. Abantu batheleleka kakhulu ngokuthintana noketshezi lomzimba, uketshezi kanye nendle yeziguli noma izilwane ezithelelekile, futhi izimpawu zemitholampilo ikakhulukazi umkhuhlane ophumayo, ukopha kanye nomonakalo wezitho eziningi. I-EHF inezinga eliphezulu lokufa elingu-50%-90%. Njengamanje, izindlela zokuxilonga i-Ebolavirus ikakhulukazi ziyizivivinyo zelebhu, okuhlanganisa izici ezimbili: ukutholwa kwe-etiological kanye nokutholwa kwe-serological. Ukutholwa kwe-etiological kufaka phakathi ukuthola ama-antigen egciwane kumasampula egazi yi-ELISA, ukuthola ama-nucleic acid ngezindlela zokukhulisa njenge-RT-PCR, njll., kanye nokusebenzisa amaseli i-Vero, i-Hela, njll. ukuze kuhlukaniswe igciwane futhi kukhuliswe. Ukutholwa kwe-serological kufaka phakathi ukuthola ama-antibodies e-Serum ethize IgM ngokubamba i-ELISA, kanye nokuthola ama-antibodies e-Serum ethize IgG yi-ELISA, i-immunofluorescence, njll.

Amapharamitha Obuchwepheshe

Isitoreji

≤30℃

Isikhathi sokuphelelwa yisikhathi Izinyanga ezingu-12
Uhlobo Lwesifanekiso i-serum, iplaamasampula e-sma
CV ≤5.0%
I-LoD Amakhophi angu-500/μL
Izinsimbi Ezisebenzayo Kusebenza ku-reagent yokuthola uhlobo I:

Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time,

I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5,

Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.),

Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer),

I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.),

Uhlelo lwe-BioRad CFX96 Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 Real-Time PCR.

Kusebenza ku-reagent yokuthola uhlobo lwesibili:

I-EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

Ukugeleza Komsebenzi

I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ukukhishwa kufanele kwenziwe ngokwemiyalelo, futhi ivolumu yesampula ekhishwe ingu-200μL kanti ivolumu yokususa enconywayo ingu-80μL.


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