I-Zaire eyomisiwe ngeqhwa kanye ne-Sudan Ebolavirus Nucleic Acid
Igama lomkhiqizo
I-HWTS-FE035-Ikhithi Yokuthola I-Nucleic Acid Eyomisiwe e-Zaire nase-Sudan Ebolavirus (i-Fluorescence PCR)
I-Epidemiology
I-Ebolavirus ingeye-Filoviridae, okuyigciwane le-RNA elingenayo imigqa eyodwa elingakahlukaniswa. Ama-virus ayimicu emide enobude obumaphakathi be-virion obungu-1000nm kanye nobubanzi obungu-100nm. I-genome ye-Ebolavirus iyi-RNA engenayo imigqa engakahlukaniswa enobukhulu obungu-18.9kb, equkethe amaprotheni ayi-7 ahlelekile kanye neprotheni eyodwa engeyona eyakhekile. I-Ebolavirus ingahlukaniswa ngezinhlobo ezifana ne-Zaire, i-Sudan, i-Bundibugyo, i-Tai Forest kanye ne-Reston. Phakathi kwazo, uhlobo lwe-Zaire kanye nohlobo lwe-Sudan kubikwe ukuthi lubangela ukufa kwabantu abaningi ngenxa yokutheleleka. I-EHF (i-Ebola Hemorrhagic Fever) yisifo esithathelwanayo esibangelwa ukopha okubukhali esibangelwa yi-Ebolavirus. Abantu batheleleka kakhulu ngokuthintana noketshezi lomzimba, uketshezi kanye nendle yeziguli noma izilwane ezithelelekile, futhi izimpawu zemitholampilo ikakhulukazi umkhuhlane ophumayo, ukopha kanye nomonakalo wezitho eziningi. I-EHF inezinga eliphezulu lokufa elingu-50%-90%. Njengamanje, izindlela zokuxilonga i-Ebolavirus ikakhulukazi ziyizivivinyo zelebhu, okuhlanganisa izici ezimbili: ukutholwa kwe-etiological kanye nokutholwa kwe-serological. Ukutholwa kwe-etiological kufaka phakathi ukuthola ama-antigen egciwane kumasampula egazi yi-ELISA, ukuthola ama-nucleic acid ngezindlela zokukhulisa njenge-RT-PCR, njll., kanye nokusebenzisa amaseli i-Vero, i-Hela, njll. ukuze kuhlukaniswe igciwane futhi kukhuliswe. Ukutholwa kwe-serological kufaka phakathi ukuthola ama-antibodies e-Serum ethize IgM ngokubamba i-ELISA, kanye nokuthola ama-antibodies e-Serum ethize IgG yi-ELISA, i-immunofluorescence, njll.
Amapharamitha Obuchwepheshe
| Isitoreji | ≤30℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | i-serum, iplaamasampula e-sma |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-500/μL |
| Izinsimbi Ezisebenzayo | Kusebenza ku-reagent yokuthola uhlobo I: Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 Real-Time PCR. Kusebenza ku-reagent yokuthola uhlobo lwesibili: I-EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza Komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ukukhishwa kufanele kwenziwe ngokwemiyalelo, futhi ivolumu yesampula ekhishwe ingu-200μL kanti ivolumu yokususa enconywayo ingu-80μL.







