I-Zaire eyomisiwe yiqhwa kanye ne-Sudan Ebolavirus Nucleic Acid
Igama lomkhiqizo
I-HWTS-FE035-Freeze-dried Zaire kanye neSudan Ebolavirus Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Ebolavirus ingeye-Filoviridae, okuyigciwane le-RNA elinomucu owodwa ongahlukaniswanga. Amagciwane ayimicu emide enobude obujwayelekile be-virion obuyi-1000nm kanye nobubanzi obuyi-100nm. I-genome ye-Ebolavirus iyi-RNA ye-negative-strand engahlukanisiwe enosayizi ongu-18.9kb, efaka ikhodi yamaphrotheni esakhiwo angu-7 kanye nephrotheni eyodwa engahlelekile. I-Ebolavirus ingahlukaniswa ngezinhlobo ezifana neZaire, Sudan, Bundibugyo, Tai Forest kanye neReston. Phakathi kwazo, uhlobo lweZaire kanye nohlobo lwaseSudan kubikwe ukuthi zibangele ukufa kwabantu abaningi ngenxa yokutheleleka. I-EHF (Ebola Hemorrhagic Fever) isifo esithathelwanayo esopha kakhulu esibangelwa i-Ebolavirus. Abantu batheleleka kakhulu ngokuthintana noketshezi lomzimba, uketshezi kanye nendle yeziguli noma izilwane ezingenwe yilesi sifo, futhi ukubonakaliswa kwemitholampilo ikakhulukazi imfiva ephumela ngaphandle, ukopha kanye nokulimala kwezitho eziningi. I-EHF inezinga eliphezulu lokufa lika-50% -90%. Njengamanje, izindlela zokuxilonga i-Ebolavirus ziwukuhlolwa kwaselabhorethri, okuhlanganisa izici ezimbili: ukutholwa kwe-etiological kanye nokutholwa kwe-serological. Ukutholwa kwe-etiological kuhlanganisa ukuthola ama-antigen egciwane kumasampula egazi nge-ELISA, ukuthola ama-nucleic acid ngezindlela zokukhulisa njenge-RT-PCR, njll., nokusebenzisa amaseli i-Vero, i-Hela, njll. ukuzehlukanisa negciwane namasiko. Ukutholwa kwe-Serological kufaka phakathi ukuthola amasosha omzimba e-Serum ethize e-IgM ngokubamba i-ELISA, kanye nokuthola amasosha omzimba athile e-IgG nge-ELISA, i-immunofluorescence, njll.
Imingcele Yezobuchwepheshe
| Isitoreji | ≤30℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | serum, plaamasampula e-sma |
| CV | ≤5.0% |
| LoD | 500 Amakhophi/μL |
| Izinsimbi Ezisebenzayo | Isebenza ekuthayipheni i-reagent yokuthola i-I: I-Applied Biosystems 7500 Real-Time PCR Systems, I-QuantStudio®5 Amasistimu e-PCR wesikhathi sangempela, I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), I-LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), I-BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. Kusebenza kuhlobo lwe-II esebenzayo yokuthola: EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ukukhishwa kufanele kwenziwe ngokulandela imiyalelo, futhi umthamo wesampula okhishiwe ngu-200μL futhi ivolumu ye-elution enconywayo ngu-80μL.
