Iqembu B le-Streptococcus Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-UR027-Iqembu B le-Streptococcus Nucleic Acid (i-Fluorescence PCR)
Ikhithi Yokuthola I-Streptococcus Nucleic Acid Eyomisiwe Yeqembu B le-HWTS-UR028 (i-Fluorescence PCR)
Isitifiketi
CE, i-FDA
I-Epidemiology
I-Group B Streptococcus (GBS), eyaziwa nangokuthi i-streptococcus agalactiae, iyigciwane elisebenzisa i-gram-positive elivame ukuhlala ezindaweni eziphansi zesisu kanye ne-urogenital zomzimba womuntu. Cishe u-10%-30% wabesifazane abakhulelwe bane-GBS vaginal survival.
Abesifazane abakhulelwe basengozini yokutheleleka yi-GBS ngenxa yezinguquko endaweni yangaphakathi yendlela yokuzala ngenxa yezinguquko emazingeni ama-hormone emzimbeni, okuzobangela imiphumela emibi yokukhulelwa njengokubeletha ngaphambi kwesikhathi, ukuqhekeka kwezicubu ngaphambi kwesikhathi, kanye nokuzalwa komntwana eseshonile, futhi kungaholela nasekuthelelekeni kwe-puerperal kwabesifazane abakhulelwe.
I-streptococcus yeqembu le-B losana olusanda kuzalwa ihlotshaniswa nokutheleleka kwangaphambi kokuzalwa futhi iyimbangela ebalulekile yezifo ezithathelwanayo ezinzima njenge-sepsis yezingane ezisanda kuzalwa kanye ne-meningitis. 40%-70% omama abangenwe yi-GBS bazodlulisela i-GBS ezinganeni zabo ezisanda kuzalwa ngesikhathi sokubeletha ngomsele wokuzalwa, okubangela izifo ezithathelwanayo ezinkulu zezingane ezisanda kuzalwa ezifana ne-sepsis yezingane ezisanda kuzalwa kanye ne-meningitis. Uma izingane ezisanda kuzalwa zine-GBS, cishe u-1%-3% uzoba nokutheleleka okungenela ngokushesha, okungu-5% kuzoholela ekufeni.
Isiteshi
| I-FAM | Inhloso ye-GBS |
| I-VIC/HEX | Ukulawula kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ ebumnyameni; I-Lyophilization: ≤30℃ ebumnyameni |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Ukukhishelwa Kwezitho Zangasese Nasezinqeni |
| Ct | ≤38 |
| CV | ≤5.0％ |
| I-LoD | 1 × 103Amakhophi/mL |
| Izinhlobo Ezingaphansi Ezimboza | Thola ama-serotype e-streptococcus eqembu B (I a, I b, I c, II, III, IV, V, VI, VII, VIII, IX kanye ne-ND) bese imiphumela yonke ilungile. |
| Ukucaciswa | Thola amanye amasampula e-genital tract kanye ne-rectal swab njenge-candida albicans, i-trichomonas vaginalis, i-chlamydia trachomatis, i-ureaplasma urealyticum, i-neisseria gonorrhoeae, i-mycoplasma hominis, i-mycoplasma genitalium, i-herpes simplex virus, i-human papilloma virus, i-lactobacillus, i-gardnerella vaginalis, i-staphylococcus aureus, i-national negative reference N1-N10 (i-streptococcus pneumoniae, i-streptococcus pyogenes, i-streptococcus thermophilus, i-streptococcus mutans, i-streptococcus pyogenes, i-lactobacillus acidophilus bacillus, i-lactobacillus reuteri, i-escherichia coli DH5α, i-candida albicans) kanye ne-human genomic DNA, imiphumela yonke ayimibi kwi-group B streptococcus. |
| Izinsimbi Ezisebenzayo | Ingafanelana namathuluzi e-PCR e-fluorescent ajwayelekile emakethe. Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Izinhlelo ze-PCR ze-ABI 7500 Zesikhathi Sangempela Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR ze-LightCycler®480 Zesikhathi Sangempela Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler |
Isixazululo se-PCR Esiphelele
