Ukuhlolwa kwe-HCV Genotype

Isithombe Esifakiwe Sokuhlonza Izakhi Zofuzo ze-HCV

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola i-genotyping ye-hepatitis C virus (HCV) subtypes 1b, 2a, 3a, 3b kanye ne-6a kumasampula e-serum/plasma emtholampilo ye-hepatitis C virus (HCV). Isiza ekuxilongweni nasekwelapheni iziguli ze-HCV.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama Lomkhiqizo

Ikhithi Yokuthola Ufuzo lwe-HWTS-HP004-HCV (i-Fluorescence PCR)

I-Epidemiology

Igciwane le-Hepatitis C (i-HCV) lingelomndeni we-flaviviridae, futhi i-genome yalo iyi-RNA eyodwa enhle, eguquguquka kalula. Leli gciwane likhona kuma-hepatocytes, ama-leukocyte e-serum kanye ne-plasma yabantu abanegciwane. Izakhi zofuzo ze-HCV zisengozini yokuguqulwa futhi zingahlukaniswa okungenani zibe izinhlobo ze-genotypes ezi-6 kanye nezinhlobo eziningi. Izinhlobo ze-genotypes ezahlukene ze-HCV zisebenzisa izindlela ezahlukene zokwelapha ze-DAA kanye nezifundo zokwelapha. Ngakho-ke, ngaphambi kokuba iziguli zelashwe ngokwelashwa kwe-DAA antiviral, i-genotype ye-HCV kumele itholakale, futhi ngisho nasezigulini ezinesifo sohlobo 1, kubalulekile ukuhlukanisa ukuthi ngabe uhlobo 1a noma uhlobo 1b.

Isiteshi

I-FAM	Uhlobo 1b, Uhlobo 2a
I-ROX	Uhlobo 6a, Uhlobo 3a
I-VIC/HEX	Ukulawula Kwangaphakathi, Uhlobo 3b

Amapharamitha Obuchwepheshe

Isitoreji	≤-18℃ Ebumnyameni
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-9
Uhlobo Lwesifanekiso	I-Serum, i-Plasma
Ct	≤36
CV	≤5.0％
I-LoD	200 IU/mL
Ukucaciswa	Sebenzisa le khithi ukuthola amanye amasampula egciwane noma amabhaktheriya afana nalawa: i-human cytomegalovirus, igciwane le-Epstein-Barr, igciwane le-human immunodeficiency, igciwane le-hepatitis B, igciwane le-hepatitis A, i-syphilis, igciwane le-herpes lohlobo 6, igciwane le-herpes simplex lohlobo 1, i-simplex igciwane le-Herpes lohlobo 2, igciwane le-influenza A, i-Propionibacterium acnes, i-Staphylococcus aureus, i-Candida albicans, njll. Imiphumela yonke ayimibi.
Izinsimbi Ezisebenzayo	Ingafanelana namathuluzi e-PCR e-fluorescent ajwayelekile emakethe. Izinhlelo ze-PCR ze-ABI 7500 Zesikhathi Sangempela Izinhlelo ze-PCR ze-ABI 7500 ze-Fast Real-Time Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR ze-LightCycler®480 Zesikhathi Sangempela Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR