Igciwane le-Hepatitis A
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-HP005 Hepatitis A Virus Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Igciwane le-Hepatitis A (HAV) liyimbangela eyinhloko ye-hepatitis ebangelwa yigciwane eliyingozi. Leli gciwane liyigciwane le-RNA elilodwa eline-positive-sense futhi lingowohlobo lwe-Hepadnavirus lomndeni i-Picornaviridae. Igciwane le-Hepatitis A, elidluliselwa kakhulu ngomzila wendle-oral, elimelana nokushisa, ama-acid, kanye nezinyibilikisi eziningi eziphilayo, lingaphila isikhathi eside ezikhwameni, emanzini, enhlabathini, noma emanzini angaphansi kolwandle [1-3]. Lidluliselwa ngokuba nokudla noma amanzi angcolile, noma lidluliselwa ngqo kusuka kumuntu kuya komunye. Ukudla okuhlobene ne-HAV kufaka phakathi ama-oyster nama-clam, ama-strawberry, ama-raspberry, ama-blueberry, ama-date, imifino eluhlaza, kanye notamatisi owomile kancane [4‒6].
Isiteshi
| I-FAM | I-HAV nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Uketshezi: izinyanga ezingu-9, i-Lyophilized: izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-serum/isihlalo |
| Tt | ≤38 |
| CV | ≤5.0% |
| I-LoD | Amakhophi ama-2/μL |
| Ukucaciswa | Sebenzisa amakhithi ukuhlola amanye amagciwane e-hepatitis njenge-hepatitis B, C, D, E, enterovirus 71, igciwane le-coxsackie, igciwane le-Epstein-Barr, i-norovirus, i-HIV kanye ne-genome yomuntu. Akukho ukusabela okuxubile. |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola I-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Amasampula e-serum
Inketho 1.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Kufanele ikhishwe ngokwemiyalelo. Umthamo wokuhlanza onconywayo ungu-80µL.
Inketho 2.
I-TIANamp Virus DNA/RNA Kit (YDP315-R) eyenziwe yi-Tiangen Biotech (Beijing) Co., Ltd. Kufanele ikhishwe ngokwemiyalelo. Umthamo wesampula okhishwe ngu-140μL. Umthamo wokuhlunga onconywayo ngu-60μL.
2.Amasampula Endle
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Kufanele ikhishwe ngokwemiyalelo. Umthamo wokuhlanza onconywayo ungu-80µL.
