I-Hepatitis A Virus
Igama lomkhiqizo
I-HWTS-HP005 Hepatitis A Virus Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Hepatitis A virus (HAV) iyimbangela eyinhloko ye-acute viral hepatitis.Leli gciwane liyigciwane le-RNA elinomucu owodwa futhi lingelohlobo lwe-Hepadnavirus lomndeni we-Picornaviridae.Igciwane le-Hepatitis A, elisakazwa kakhulu ngomzila we-fecal-oral, elimelana nokushisa, ama-acids, nezinyibilikisi eziningi eziphilayo, lingaphila isikhathi eside ku-shellfish, emanzini, enhlabathini, noma emanzini olwandle [1-3].Sisakazwa ngokuba nokudla noma amanzi angcolile, noma atheleleke ngokuqondile kusuka kumuntu kuya kumuntu.Ukudla okuhlotshaniswa ne-HAV kufaka phakathi ama-oyster nama-clams, ama-strawberry, ama-raspberries, amajikijolo aluhlaza, amadethi, imifino enamahlamvu aluhlaza, notamatisi omiswe kancane[4-6].
Isiteshi
| FAM | I-HAV nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Uketshezi: izinyanga eziyi-9, i-Lyophilized: izinyanga eziyi-12 |
| Uhlobo Lwesifanekiso | I-Serum/indle |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 2 Amakhophi/μL |
| Ukucaciswa | Sebenzisa amakhithi ukuhlola amanye amagciwane e-hepatitis B, C, D, E, enterovirus 71, igciwane le-coxsackie, igciwane le-Epstein-Barr, i-norovirus, i-HIV ne-genome yomuntu.Ayikho i-cross-reactivity. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela I-SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Amasampula eSerum
Inketho 1.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Kufanele ikhishwe ngokulandela imiyalelo.Ivolumu ye-elution enconyiwe ngu-80µL.
Inketho yesi-2.
I-TIANamp Virus DNA/RNA Kit (YDP315-R) ekhiqizwa i-Tiangen Biotech (Beijing) Co., Ltd. Kufanele ikhishwe ngokuvumelana neziyalezo.Ivolumu yesampula ekhishiwe ingu-140μL.Ivolumu ye-elution enconyiwe ngu-60µL.
2.Amasampula endle
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Kufanele ikhishwe ngokulandela imiyalelo.Ivolumu ye-elution enconyiwe ngu-80µL.
