I-Hepatitis B I-Virus DNA Dianitative Fluorescence
Igama lomkhiqizo
I-HWTS-HP015 Hepatitis B Virus DNA I-DNA I-FLEORESTICENICECECECECEOstiC KIT (Fluorescence PCR)
I-Epidemiology
I-Hepatitis B yisifo esidalwa yi-hepatitis B Virus (HBV), ikakhulukazi esibonakala ngokulimala kwesibindi, futhi singadala ukulimala kwezitho eziningi. Iziguli zeHepatitis B zibonakaliswa ngokomtholampilo njengokukhathala, ukulahlekelwa yisifiso sokudla, umkhawulo ophansi noma i-edema ejwayelekile, ne-hepatomegaly ngenxa yomsebenzi wesibindi okhubazekile. Amaphesenti amahlanu abantu abadala abathelelekile kanye nama-95% abantu abatheleleke ngokuqondile abakwazi ukusula ngempumelelo i-HBV ephikelelayo, kanti ezinye izifo ezingamahlalakhona zigcina zikhule zibe yi-Cirrhosis yesibindi ne-Hepatocellular Carcinoma[1-4].
Insungubezi
| -Yenza | I-HBV-DNA |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | i-serum entsha, i-plasma |
| Tt | ≤42 |
| CV | ≤5.0% |
| Indlu edayisa insimbi | I-IU / ML |
| Ukucacisa | Imiphumela yokucaciswa ikhombisa ukuthi wonke amacala angama-50 e-HBV DNA ama-HBV DNA ama-serum amasampula awalungile; Imiphumela yokuhlolwa kwe-Cross-Resivity Test ikhombisa ukuthi akukho ukusabela kwe-cross phakathi kwamanye ama-kit namanye amagciwane (hav, HCV, DFV, i-HIV) ngokutholwa kwe-nucleic acid ngamasampula egazi, kanye ne-genomes yabantu. |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 uhlelo lwe-real-time PCR Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®5 Systems real-time PCREMS ISlan-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.) I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Plus real-time PCCRECT SYPELY (FQD-96A, Hangzhou Bioer Technology) I-MA-6000 Real-Time Cycler Cycler yesikhathi sangempela (Suzhou Molarray Co, Ltd.) I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
I-reaglent enconyiwe I-realent: I-Macro & Micro-Test Virus DNA / RNA KIT (HWTS-3017) (engasetshenziswa nge-macro & micro-eq Co, LTD .. Ukukhishwa kufanele kwenziwe ngokuya ngencwadi yokufundisa, ivolumu yesampula ekhishwe I-300μL, futhi ivolumu yokuqedwa enconyiwe lingu-70μL.
