I-Hepatitis B Virus DNA Quantitative Fluorescence
Igama lomkhiqizo
Ikhithi Yokuhlola I-HWTS-HP015 Hepatitis B Virus DNA Quantitative Fluorescence Diagnostic (i-Fluorescence PCR)
I-Epidemiology
I-Hepatitis B yisifo esibangelwa yigciwane le-hepatitis B (HBV), elibonakala kakhulu ngezilonda zokuvuvukala kwesibindi, futhi lingabangela ukulimala kwezitho eziningi. Iziguli ze-Hepatitis B zibonakala ngokwezokwelapha njengokukhathala, ukulahlekelwa yisifiso sokudla, ukuvuvukala kwemilenze engezansi noma okuvamile, kanye ne-hepatomegaly ngenxa yokusebenza kwesibindi okungasebenzi kahle. Amaphesenti amahlanu abantu abadala abanegciwane kanye nama-95% abantu abanegciwane eliqondile abakwazi ukuqeda i-HBV ngempumelelo, okuholela ekuthelelekeni ngegciwane okuqhubekayo, kanti ezinye izifo ezingamahlalakhona ekugcineni ziphenduka i-cirrhosis yesibindi kanye ne-hepatocellular carcinoma.[1-4].
Isiteshi
| I-FAM | I-HBV-DNA |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | i-serum entsha, i-Plasma |
| Tt | ≤42 |
| CV | ≤5.0% |
| I-LoD | 5 IU/mL |
| Ukucaciswa | Imiphumela yokunemba ikhombisa ukuthi wonke amacala angu-50 e-HBV DNA negative serum amasampula e-serum anempilo awanawo umphumela omuhle; imiphumela yokuhlolwa kwe-cross-reactivity ikhombisa ukuthi akukho ukusabela okuhlangene phakathi kwale kit namanye amagciwane (i-HAV, i-HCV, i-DFV, i-HIV) kokutholwa kwe-nucleic acid ngamasampula egazi, kanye nama-genome abantu. |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test Virus DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ukukhipha kufanele kwenziwe ngokwencwadi yemiyalelo, ivolumu yesampula ekhishwe ingu-300μL, kanti ivolumu yokususa enconyiwe ingu-70μL.
