I-Hepatitis B Virus DNA Quantitative Fluorescence
Igama lomkhiqizo
I-HWTS-HP015 Hepatitis B Virus DNA Quantitative Fluorescence Diagnostic Kit (Fluorescence PCR)
I-Epidemiology
I-Hepatitis B yisifo esibangelwa igciwane le-hepatitis B (HBV), elibonakala kakhulu ngokuvuvuka kwesibindi, futhi lingabangela ukulimala kwezitho eziningi.Iziguli ze-Hepatitis B zibonakaliswa ngokomtholampilo njengokukhathala, ukulahlekelwa ukudla, umkhawulo ophansi noma i-edema evamile, kanye ne-hepatomegaly ngenxa yokungasebenzi kahle kwesibindi.Amaphesenti amahlanu abantu abadala abangenwe yileli gciwane kanye nama-95% abantu abatheleleke ngokuqondile abakwazi ukuyisula ngokuphumelelayo i-HBV, okuholela ekuthelelekeni ngegciwane okuqhubekayo, kanti ezinye izifo ezingelapheki zigcina ziba yi-cirrhosis yesibindi kanye ne-hepatocellular carcinoma.[1-4].
Isiteshi
| FAM | I-HBV-DNA |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | i-serum entsha, i-Plasma |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 5 IU/mL |
| Ukucaciswa | Imiphumela ecacile ibonisa ukuthi wonke amacala angu-50 we-HBV DNA enempilo yamasampula e-serum anegethivu;imiphumela yokuhlolwa kwe-cross-reactivity ibonisa ukuthi akukho ukusabela okuphambene phakathi kwale khithi namanye amagciwane (HAV, HCV, DFV, HIV) ukuze kutholwe i-nucleic acid ngamasampula egazi, nezakhi zofuzo zomuntu. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems I-SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ukukhishwa kufanele kwenziwe ngokuya ngebhukwana lemiyalo, ivolumu yesampula ekhishiwe ingu-300μL, futhi ivolumu ye-elution enconyiwe ingu-70μL.
