Ufuzo lwegciwane le-Hepatitis B
Igama lomkhiqizo
Ikhithi Yokuthola Ufuzo Lwegciwane le-HWTS-HP002-Hepatitis B (i-PCR Ekhanyayo)
I-Epidemiology
I-Epidemiology
Isiteshi
| IsiteshiIgama | I-Reaction Buffer 1 | I-Reaction Buffer 2 |
| I-FAM | I-HBV-C | I-HBV-D |
| I-VIC/HEX | I-HBV-B | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ Ebumnyameni |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-Serum, i-Plasma |
| Ct | ≤38 |
| CV | ≤5.0％ |
| I-LoD | 1 × 102IU/mL |
| Ukucaciswa | Akukho ukusabela okuhlangene negciwane le-hepatitis C, i-human cytomegalovirus, igciwane le-Epstein-Barr, igciwane le-human immunodeficiency, igciwane le-hepatitis A, i-syphilis, igciwane le-herpes, igciwane le-influenza A, i-propionibacterium acnes (PA), njll. |
| Izinsimbi Ezisebenzayo | Ingafanelana namathuluzi e-PCR e-fluorescent ajwayelekile emakethe. Izinhlelo ze-PCR ze-ABI 7500 Zesikhathi Sangempela Izinhlelo ze-PCR ze-ABI 7500 ze-Fast Real-Time Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR ze-LightCycler®480 Zesikhathi Sangempela Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhipha kufanele kwenziwe ngokwencwadi yemiyalelo, ivolumu yesampula ekhishwe ingu200μL, futhi ivolumu yokuxazulula enconywayo ingu80μL.
I-reagent yokukhipha enconyiwe: I-Nucleic Acid Extraction noma i-Purification Reagent (YDP315). Ukukhipha kufanele kwenziwe ngokuhambisana nemiyalelo eqinile. Umthamo wesampula okhishwe ungama-200µL, kanti umthamo wokususa onconyiwe ungama-100µL.
