Igciwane le-Hepatitis B
Igama Lomkhiqizo
Ikhithi Yokuthola I-Nucleic Acid Ye-HWTS-HP001-Hepatitis B Virus (i-Fluorescence PCR)
I-Epidemiology
I-Hepatitis B yisifo esithathelwanayo esinokulimala kwesibindi kanye nezitho eziningi zomzimba okubangelwa yigciwane le-hepatitis B (HBV). Iningi labantu liba nezimpawu ezifana nokukhathala okukhulu, ukulahlekelwa yisifiso sokudla, izitho ezingezansi noma ukuvuvukala komzimba wonke, i-hepatomegaly, njll. 5% yeziguli ezindala kanye no-95% wezingane iziguli ezitheleleke ngonina azikwazi ukuhlanza igciwane le-HBV ngempumelelo ekuthelelekeni okuqhubekayo futhi ziqhubekele ekubeni yi-cirrhosis yesibindi noma i-carcinoma yeseli lesibindi eliyinhloko..
Isiteshi
| I-FAM | I-HBV-DNA |
| I-VIC (HEX) | Inkomba yangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ Ebumnyameni |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Igazi lemithambo yegazi |
| Ct | ≤33 |
| CV | ≤5.0％ |
| I-LoD | 25IU/mL |
| Ukucaciswa | Akukho ukuhlangana kwe-Cytomegalovirus, igciwane le-EB, i-HIV, i-HAV, i-Syphilis, i-Human Herpesvirus-6, i-HSV-1/2, i-Influenza A, i-Propionibacterium Acnes, i-Staphylococcus Aureus kanye ne-Candida albican. |
| Izinsimbi Ezisebenzayo | Ingafanelana namathuluzi e-PCR e-fluorescent ajwayelekile emakethe. Izinhlelo ze-PCR ze-ABI 7500 Zesikhathi Sangempela Izinhlelo ze-PCR ze-ABI 7500 ze-Fast Real-Time Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR ze-LightCycler®480 Zesikhathi Sangempela Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ama-reagent okukhipha anconyiwe: I-Macro kanye ne-Micro-TestIgciwaneI-DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ukukhipha kufanele kuqalwe ngokuya nge-IFU ye-reagent yokukhipha. Umthamo wesampula okhishwe ungama-200µL kanti umthamo wokususa onconywayo ungama-80 μL.
Ama-reagent okukhipha anconyiwe: Ama-Nucleic Acid Extraction noma ama-Purification Reagents (YDP315). Ukukhipha kufanele kuqalwe ngokuhambisana ne-IFU. Umthamo wesampula okhishwe ungama-200µL kanti umthamo wokususa onconyiwe ungama-100 μL.
