Igciwane le-Herpes simplex uhlobo 1/2, i-Trichomonal vaginitis nucleic acid
Igama lomkhiqizo
I-HWTS-UR045-Igciwane le-Herpes simplex uhlobo 1/2, ikhithi yokuthola i-nucleic acid ye-Trichomonal vaginitis (i-Fluorescence PCR)
I-Epidemiology
I-genital herpes yisifo esivamile esidluliselwa ngocansi esibangelwa yi-HSV2, esithathelwana kakhulu. Eminyakeni yamuva nje, ukwanda kwe-genital herpes kukhuphuke kakhulu, futhi ngenxa yokwanda kwemikhuba yocansi eyingozi, izinga lokutholakala kwe-HSV1 ku-genital herpes likhuphuke futhi kwabikwa ukuthi liphezulu ngamaphesenti angama-20-30%. Ukutheleleka kokuqala ngegciwane le-genital herpes ngokuvamile akubonakali ngaphandle kwezimpawu zomtholampilo ezisobala ngaphandle kwe-herpes yendawo esikhumbeni noma esikhumbeni seziguli ezimbalwa. Njengoba i-genital herpes ibonakala ngokukhishwa kwegciwane impilo yonke kanye nokuthambekela kokubuyela emuva, kubalulekile ukuhlola amagciwane ngokushesha okukhulu futhi kuvimbele ukudluliselwa kwawo.
Amapharamitha Obuchwepheshe
| Isitoreji | -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | i-swab yesilisa ye-urethra, i-swab yesibeletho yesifazane, i-swab yesitho sangasese sowesifazane |
| Ct | ≤38 |
| CV | ＜5.0% |
| I-LoD | 400Amakhophi/mL |
| Izinsimbi Ezisebenzayo | Kusebenza ku-reagent yokuthola uhlobo I: Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR. Kusebenza ku-reagent yokuthola uhlobo lwesibili: I-EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza Komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kanye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetshenziswa ne-EudemonTM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampula okhishwe ungama-200μL kanti umthamo wokulungisa onconywayo ungama-150μL.
