I-HPV16 kanye ne-HPV18
Igama lomkhiqizo
HWTS-CC001-I-HPV16 kanye ne-HPV18 Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Umdlavuza womlomo wesibeletho ungesinye sezimila eziyingozi ezivame kakhulu emgudwini wokuzala wabesifazane.Kukhonjisiwe ukuthi ukutheleleka nge-HPV okungapheli kanye nezifo eziningi kungenye yezimbangela ezinkulu zomdlavuza womlomo wesibeletho.Okwamanje kusenokuntuleka kwemithi yokwelapha esebenzayo eyamukelekayo yomdlavuza womlomo wesibeletho obangelwa i-HPV.Ngakho-ke, ukutholakala kusenesikhathi kanye nokuvimbela ukutheleleka komlomo wesibeletho okubangelwa i-HPV yizihluthulelo zokuvimbela umdlavuza womlomo wesibeletho.Ukusungulwa kwezivivinyo ezilula, eziqondile nezisheshayo zokuxilonga amagciwane kubaluleke kakhulu ekuhlonzweni komtholampilo komdlavuza womlomo wesibeletho.
Isiteshi
| Isiteshi | Uhlobo |
| FAM | I-HPV18 |
| VIC/HEX | I-HPV16 |
| CY5 | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | iseli yomlomo wesibeletho exfoliated |
| Ct | ≤28 |
| CV | ≤10.0% |
| LoD | 500 Amakhophi/mL |
| Ukucaciswa | Uma usebenzisa ikhithi ukuhlola izibonelo ezingaqondile ezingase zisabele kulokho okuhlosiwe, yonke imiphumela iba mibi, okuhlanganisa i-Ureaplasma urealyticum, Chlamydia trachomatis, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mildew, Gardnerella kanye nezinye izinhlobo ze-HPV ezingamboziwe. ikhithi. |
| Izinsimbi Ezisebenzayo | I-SLAN®-96P Real-Time PCR SystemsI-Applied Biosystems 7500 Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler. |
Ukugeleza komsebenzi
Inketho1.
I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), kufanele ikhishwe ngokuya ngemiyalo.
Inketho2.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, HWTS-3006C), kufanele ikhishwe ngokulandela imiyalelo.Ivolumu yesampula ekhishiwe ingu-200μL, futhi ivolumu ye-elution enconyiwe ingu-80µL.
Inketho3.
I-QIAamp DNA Mini Kit (51304) ekhiqizwe i-QIAGEN noma i-TIANamp Virus DNA/RNA Kit (YDP315) ekhiqizwa yi-Tiangen Biotech (Beijing) Co., Ltd., kufanele ikhishwe ngokuhambisana neziyalezo eziqinile.Ivolumu yesampula ekhishiwe ingu-200μL, futhi ivolumu ye-elution enconyiwe ingu-80µL.
