I-HPV16 kanye ne-HPV18
Igama lomkhiqizo
I-HWTS-CC001-Ikhithi Yokuthola I-Nucleic Acid ye-HPV16 kanye ne-HPV18 (i-Fluorescence PCR)
I-Epidemiology
Umdlavuza womlomo wesibeletho ungenye yezimila eziyingozi ezivame kakhulu endleleni yokuzala yabesifazane. Kuye kwabonakala ukuthi ukutheleleka kwe-HPV okuqhubekayo kanye nokutheleleka okuningi kungenye yezimbangela ezinkulu zomdlavuza womlomo wesibeletho. Okwamanje kusenokuntuleka kwezindlela zokwelapha ezisebenzayo ezamukelwa kabanzi zomdlavuza womlomo wesibeletho obangelwa yi-HPV. Ngakho-ke, ukutholakala kusenesikhathi kanye nokuvimbela ukutheleleka komlomo wesibeletho okubangelwa yi-HPV kuyizihluthulelo zokuvimbela umdlavuza womlomo wesibeletho. Ukusungulwa kokuhlolwa okulula, okuqondile nokusheshayo kokuxilongwa kwamagciwane kubaluleke kakhulu ekuxilongweni komdlavuza womlomo wesibeletho emtholampilo.
Isiteshi
| Isiteshi | Uhlobo |
| I-FAM | I-HPV18 |
| I-VIC/HEX | I-HPV16 |
| CY5 | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | iseli elikhishwe umlomo wesibeletho |
| Ct | ≤28 |
| CV | ≤10.0% |
| I-LoD | Amakhophi angu-500/mL |
| Ukucaciswa | Uma usebenzisa ikhithi ukuhlola amasampula angaqondile angase ahlangane nezinhloso zayo, yonke imiphumela iba mibi, okuhlanganisa i-Ureaplasma urealyticum, i-Chlamydia trachomatis, i-Candida albicans, i-Neisseria gonorrhoeae, i-Trichomonas vaginalis, i-Mildew, i-Gardnerella kanye nezinye izinhlobo ze-HPV ezingamboziwe yikhithi. |
| Izinsimbi Ezisebenzayo | I-SLAN®-96P Izinhlelo ze-PCR Zesikhathi SangempelaIzinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler. |
Ukugeleza Komsebenzi
Inketho 1.
I-Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8), kufanele ikhishwe ngokwemiyalelo.
Inketho 2.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, HWTS-3006C), kufanele ikhishwe ngokwemiyalelo. Ivolumu yesampula ekhishwe ingu-200μL, kanti ivolumu yokulungisa enconywayo ingu-80µL.
Inketho 3.
I-QIAamp DNA Mini Kit (51304) eyenziwe yi-QIAGEN noma i-TIANamp Virus DNA/RNA Kit (YDP315) eyenziwe yi-Tiangen Biotech (Beijing) Co., Ltd., kufanele ikhishwe ngokuhambisana nemiyalelo. Umthamo wesampula okhishwe ungama-200μL, kanti umthamo wokulungisa onconywayo ungama-80μL.
