I-Influenza B Virus Nucleic Acid Inani
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-RT140-Influenza B Virus Nucleic Acid Quantitative (i-Fluorescence PCR)
I-Epidemiology
I-Influenza, evame ukubizwa ngokuthi 'i-flu', iyisifo esithathelwanayo sokuphefumula esibangelwa yigciwane le-influenza. Sithelelana kakhulu futhi sisakazeka ikakhulukazi ngokukhwehlela nokuthimula. Ngokuvamile siqala entwasahlobo nasebusika. Kunezinhlobo ezintathu: I-Influenza A (IFV A), I-Influenza B (IFV B), kanye ne-Influenza C (IFV C), zonke ezingezomndeni wakwa-Orthomyxoviridae. Izimbangela eziyinhloko zezifo zabantu amagciwane e-Influenza A kanye ne-B, futhi angama-single-strand negative-sense, ahlukaniswe nge-RNA. Amagciwane e-Influenza B ahlukaniswe ngezigaba ezimbili ezinkulu, i-Yamagata ne-Victoria. Amagciwane e-Influenza B anokukhukhuleka kwe-antigenic kuphela, futhi agwema ukubhekwa nokusulwa kwesistimu yomzimba womuntu ngokuguqulwa kwawo. Kodwa-ke, izinga lokuvela kwegciwane le-influenza B lihamba kancane kunelegciwane le-influenza A, futhi igciwane le-influenza B lingadala nokutheleleka kwendlela yokuphefumula yomuntu futhi liholele ezifweni eziwumshayabhuqe.
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isampula ye-oropharyngeal swab |
| CV | <5.0% |
| I-LoD | Amakhophi angu-500/mL |
| Ukucaciswa | Ukusabela okuxubile: akukho ukusabela okuxubile phakathi kwale khithi kanye negciwane le-influenza A, i-adenovirus uhlobo 3, 7, i-human coronavirus SARSr-CoV, i-MERSr-CoV, i-HCoV-OC43, i-HCoV-229E, i-HCoV-HKU1, kanye ne-HCoV-NL63, i-cytomegalovirus, i-enterovirus, igciwane le-parainfluenza, igciwane le-measles, igciwane le-human metapneumovirus, igciwane le-mumps, igciwane le-respiratory syncytial type B, i-rhinovirus, i-bordetella pertussis, i-chlamydia pneumoniae, i-corynebacterium, i-escherichia coli, i-haemophilus influenzae, i-jactobacillus, i-moraxella catarrhalis, i-avirulent mycobacterium tuberculosis, i-mycoplasma pneumoniae, i-neisseria meningitidis, i-neisseria gonorrhoeae, i-pseudomonas aeruginosa, i-staphylococcus aureus, i-staphylococcus epidermidis, i-streptococcus pneumoniae, i-streptococcus pyogenes, i-streptococcus i-salivarius kanye ne-DNA ye-genomic yomuntu. |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time, Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480, Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. iyanconywa ekukhishweni kwesampula futhi izinyathelo ezilandelayo kufanele zenziwe ngokuhambisana ne-IFU ye-Kit.
