Ikhithi Yokuthola I-Legionella Pneumophila Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-RT163-Legionella Pneumophila Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
I-Legionella pneumophila iyi-coccobacillus emfushane, ene-gram-negative, ye-Legionella genus polymorphic. I-Legionella pneumophila iyi-bacterium e-facultative parasitic engahlasela i-amoeba noma ama-macrophage abantu. Ukutheleleka kwaleli gciwane kukhula kakhulu lapho kukhona ama-antibodies kanye ne-serum complement (kodwa ukuba khona kokubili akudingeki ngokuphelele). I-Legionella pneumophila iyi-pathogen ebalulekile ebangela ubhubhane kanye ne-pneumonia etholakala emphakathini kanye ne-pneumonia etholakala esibhedlela, ebangela cishe u-80% we-Legionella pneumonia. I-Legionella pneumophila ikhona kakhulu emanzini nasenhlabathini. Ukumuncwa kwamanzi angcolile kanye nenhlabathi emzimbeni womuntu ngesimo se-aerosol kungaba yindlela eyinhloko yokutheleleka kwe-Legionella. Njengamanje, izindlela eziyinhloko zokuthola nokuxilongwa kwe-Legionella pneumophila elabhorethri yi-bacterial culture kanye ne-serological assay.
Amapharamitha Obuchwepheshe
| Isitoreji | -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | isikhwehlela |
| Ct | ≤38 |
| CV | 5.0% |
| I-LoD | Amakhophi ayi-1000/μL |
| Izinsimbi Ezisebenzayo | Kusebenza ku-reagent yokuthola uhlobo I: Izinhlelo ze-Biosystems ezisetshenzisiwe 7500 Real-Time PCR,QuantStudio®5 Real-Time PCR Systems,SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.),LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, ubuchwepheshe be-Hangzhou Bioer),MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.),BioRad CFX96 Real-Time PCR System,BioRad CFX Opus 96 Real-Time PCR System. |
| Kusebenza ku-reagent yokuthola uhlobo lwesibili: I-EudemonTM AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza Komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kanye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetshenziswa ne-EudemonTM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampula okhishwe ungama-200μL kanti umthamo wokulungisa onconywayo ungama-150μL.
