I-Monkeypox Virus Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-Nucleic Acid Yegciwane Le-HWTS-OT071-Monkeypox (i-Fluorescence PCR)
Ikhithi Yokuthola I-Nucleic Acid ye-HWTS-OT078-Eyomisiwe Ngokuqandisa ye-Monkeypox Virus Nucleic Acid (i-Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
I-Monkeypox (MP) isifo esithathelwanayo esibangelwa yi-Monkeypox Virus (MPV). Lesi sifo sidluliselwa kakhulu yizilwane, futhi abantu bangatheleleka ngokulunywa yizilwane ezithelelekile noma ngokuthintana ngqo negazi, uketshezi lomzimba kanye nokuqubuka kwezilwane ezithelelekile. Leli gciwane lingadluliselwa futhi phakathi kwabantu, ikakhulukazi ngamaconsi okuphefumula ngesikhathi sokuxhumana ubuso nobuso isikhathi eside, noma ngokuthintana ngqo noketshezi lomzimba wesiguli noma izinto ezingcolile.
Izimpawu zomtholampilo zokutheleleka nge-monkeypox kubantu zifana neze-smallpox, ngokuvamile ngemva kwesikhathi sokufukamela sezinsuku ezingu-12, kuvela umkhuhlane, ikhanda elibuhlungu, ubuhlungu bemisipha nomhlane, ama-lymph node akhuphukile, ukukhathala nokungakhululeki. Ukuqubuka kuvela ngemva kwezinsuku ezingu-1-3 zomkhuhlane, ngokuvamile kuqala ebusweni, kodwa nakwezinye izingxenye. Inkambo yesifo ngokuvamile ihlala amasonto angu-2-4, kanti izinga lokufa lingu-1%-10%. I-Lymphadenopathy ingenye yomehluko omkhulu phakathi kwalesi sifo ne-smallpox.
Isiteshi
| Isiteshi | I-Monkeypox |
| I-FAM | Igciwane le-Monkeypox i-MPV-1 gene |
| I-VIC/HEX | Igciwane le-Monkeypox i-MPV-2 gene |
| I-ROX | / |
| CY5 | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ Ebumnyameni; I-Lyophilized: ≤30℃ Ebumnyameni |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Uketshezi Lokuqubuka, I-Nasopharyngeal Swab, I-throat Swab, I-Serum |
| Ct | ≤38 |
| CV | ≤5.0％ |
| I-LoD | Amakhophi angu-200/mL |
| Ukucaciswa | Akukho ukuhlangana kwe-reactive negciwane le-Smallpox, igciwane le-Cowpox, igciwane le-Vaccinia, igciwane le-Herpes simplex, njll. Akukho ukuhlangana kwe-reactive nezinye izifo ezibangela isifo sokuqubuka. Akukho ukuhlangana kwe-reactive ne-DNA yomuntu ye-genomic. |
| Izinsimbi Ezisebenzayo | Ingafanelana namathuluzi e-PCR e-fluorescent ajwayelekile emakethe. Izinhlelo ze-PCR ze-ABI 7500 Zesikhathi Sangempela Izinhlelo ze-PCR ze-QuantStudio® 5 Zesikhathi Sangempela Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler |
Isixazululo se-PCR Esiphelele
