I-Mycobacterium Tuberculosis INH Ukuguqulwa
Igama lomkhiqizo
I-HWTS-RT137 Mycobacterium Tuberculosis INH Mutation Detection Kit (Ijika Elincibilikayo)
I-Epidemiology
I-Mycobacterium tuberculosis, kafushane nje njenge-Tubercle bacillus (TB), igciwane le-pathogenic elidala isifo sofuba. Njengamanje, imithi elwa nesifo sofuba evame ukusetshenziswa yomugqa wokuqala ihlanganisa i-INH, i-rifampicin ne-hexambutol, njll. Imithi yomugqa wesibili yokulwa nesifo sofuba ihlanganisa i-fluoroquinolones, i-amikacin kanye ne-kanamycin, njll. Imithi emisha ethuthukisiwe yi-linezolid, i-bedaquiline ne-delamani, njll. Nokho, ngenxa yokusetshenziswa okungalungile kwezidakamizwa ze-anti-tuberculosis kanye nesakhiwo se-tuberculosis cell, i-tuberculosis cell structure, i-tuberculosis kanye ne-tuberculosis. Isifo sofuba i-mycobacterium siqala ukumelana nemithi elwa nesifo sofuba, okuletha izinselele ezinkulu ekuvimbeleni nasekwelapheni isifo sofuba.
Isiteshi
| FAM | I-MP nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | isikhwehlela |
| CV | ≤5% |
| LoD | Umkhawulo wokuthola amabhaktheriya ohlobo lwasendle lwe-INH ngu-2x103 bacteria/mL, futhi umkhawulo wokutholwa kwamagciwane aguqukile ngu-2x103 bacteria/mL. |
| Ukucaciswa | a. Akukho ukusabela okuphambene phakathi kofuzo lomuntu, amanye amagciwane angenayo i-mycobacteria kanye ne-pneumonia atholwe yile khithi.b. Izindawo eziguqukayo zezinye izakhi zofuzo ezimelana nezidakamizwa kuhlobo lwasendle lwe-Mycobacterium tuberculosis, njengesifunda esinquma ukumelana kofuzo lwe-rifampicin rpoB, zitholiwe, futhi imiphumela yokuhlolwa ayizange ibonise ukumelana ne-INH, ebonisa ukuthi akukho ukuphinda kusebenze okuphambene. |
| Izinsimbi Ezisebenzayo | SLAN-96P Real-Time PCR SystemsI-BioRad CFX96 Real-Time PCR SystemsI-LightCycler480®Isistimu ye-PCR Yesikhathi Sangempela |
Ukugeleza komsebenzi
Uma usebenzisa i-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nge-Jiangsu Macro & Micro-Test Med-Tech Extraμ, engeza inqubo ye-negative Med-Tech0μ, Ltd. isampula yesikhwehlela okufanele ihlolwe ngokulandelana, bese wengeza u-10μL wokulawula kwangaphakathi ngokuhlukene ekulawuleni okunegethivu, isampula yesikhwehlela esicutshunguliwe ukuze sihlolwe, futhi izinyathelo ezilandelayo kufanele zenziwe ngokuqinile ngokwemiyalelo yokukhipha. Ivolumu yesampula ekhishiwe ingu-200μL, futhi ivolumu ye-lution enconyiwe ingu-100μL.
