Ukuguqulwa kwe-Mycobacterium Tuberculosis INH
Igama lomkhiqizo
Ikhithi Yokuthola Ukuguquka Kwe-HWTS-RT137 Mycobacterium Tuberculosis INH (Melting Curve)
I-Epidemiology
I-Mycobacterium tuberculosis, efushaniswa ne-Tubercle bacillus (TB), yigciwane elibangela isifo sofuba. Njengamanje, imithi yokuqala yokulwa nesifo sofuba esetshenziswa kakhulu ifaka phakathi i-INH, i-rifampicin ne-hexambutol, njll. Imithi yesibili yokulwa nesifo sofuba ifaka phakathi i-fluoroquinolones, i-amikacin ne-kanamycin, njll. Imithi emisha ethuthukisiwe yi-linezolid, i-bedaquiline ne-delamani, njll. Kodwa-ke, ngenxa yokusetshenziswa okungalungile kwemithi yokulwa nesifo sofuba kanye nezici zesakhiwo sodonga lweseli se-mycobacterium tuberculosis, i-mycobacterium tuberculosis ithuthukisa ukumelana nemithi elwa nesifo sofuba, okuletha izinselele ezinkulu ekuvimbeleni nasekwelapheni isifo sofuba.
Isiteshi
| I-FAM | i-asidi ye-nucleic ye-MP |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | isikhwehlela |
| CV | ≤5% |
| I-LoD | Umkhawulo wokutholwa kwamabhaktheriya e-INH ohlobo lwasendle ungama-bacteria angu-2x103/mL, kanti umkhawulo wokutholwa kwamabhaktheriya aguquliwe ungama-bacteria angu-2x103/mL. |
| Ukucaciswa | a. Akukho ukusabela okuhlangene phakathi kwe-genome yabantu, ezinye izifo ze-mycobacteria ezingenayo i-tuberculous kanye ne-pneumonia ezitholwe yile kit.b. Izindawo zokuguquka kwezakhi zofuzo ezingamelani nemithi ku-Mycobacterium tuberculosis yohlobo lwasendle, njengesifunda esinquma ukumelana kwezakhi zofuzo i-rifampicin rpoB, zatholakala, futhi imiphumela yokuhlolwa ayizange ibonise ukumelana ne-INH, okubonisa ukuthi akukho ukusabela okuphambene. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96PIzinhlelo ze-PCR ze-BioRad CFX96 Real-TimeI-LightCycler480®Uhlelo lwe-PCR Lwesikhathi Sangempela |
Ukugeleza Komsebenzi
Uma usebenzisa i-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ukuze kukhishwe, engeza u-200μL wesampula yesikhwehlela esingesihle kanye nesampula yesikhwehlela esicutshunguliwe ukuze sihlolwe ngokulandelana, bese ufaka u-10μL wesilawuli sangaphakathi ngokwehlukana kusampula yesikhwehlela esingesihle, esicutshunguliwe ukuze sihlolwe, futhi izinyathelo ezilandelayo kufanele zenziwe ngokuqinile ngokwemiyalelo yokukhipha. Ivolumu yesampula ekhishwe ingu-200μL, kanti ivolumu yokususa enconywayo ingu-100μL.
