I-Mycobacterium Tuberculosis InH ukuguqulwa
Igama lomkhiqizo
I-HWTS-RT137 MyCobacterium Tuberculosis Innist Retation Detection Detection Kit (ukuncibilika ijika)
I-Epidemiology
I-Mycobacterium Tuberculosis, kungekudala njenge-tubercle bacillus (TB), i-bacterium ye-pathogenic ebangela isifo sofuba. Njengamanje, izidakamizwa ezisetshenziswayo ezisetshenziswayo ezisetshenziswayo ezisetshenziswayo ezisetshenziswayo zifaka i-INH, Rifampicin neHexambutol, njll. Izidakamizwa ze-anti-tuberculosos zesibili zifaka ama-fluoroquicolones, i-amikacin ne-kanamycin, njll. Izidakamizwa ezintsha ezithuthukile ziyi-linezolid, e-bedaquiline kanye ne-desamani, njll . Noma kunjalo, ngenxa yokusetshenziswa okungalungile kwesifo sofuba Izidakamizwa kanye nezimpawu zokwakheka kodonga lweseli lwe-Mycobacterium Tuberculosis, i-MyCobacterium Tuberculosis ithuthukisa ukumelana nezidakamizwa ezidakamizweni ze-anti-soturculosis, okuletha izinselelo ezinkulu ekuvinjelweni nasekwelashweni kwesifo sofuba.
Insungubezi
| -Yenza | I-MP nucleic acid |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | sputum |
| CV | ≤5% |
| Indlu edayisa insimbi | Umkhawulo wokutholwa we-Wild-Type AT Bacterium yi-2x103 bacteria / ml, kanye nomkhawulo wokutholwa kwamagciwane aguqukayo yi-2x103 bacteria / ml. |
| Ukucacisa | a. Akukho ukusabela kwesiphambano phakathi kwe-genome yabantu, amanye ama-mocobacteria angenakugwetshwa ama-mycobacteria kanye nama-pneumonia pathogens atholwe yile khithi.b. Izindawo zokuguqulwa kwezinye izinhlobo zofuzo ezingamelana nezidakamizwa e-Mycobacterium Tuberculosis, njengokumelana nokuvinjwa kwesifunda iRifampicin RPOB Gene, kwatholakala, futhi imiphumela yokuhlola ayikhombisi ukumelana ne-INH, okukhombisa ukungabi nakuphikwa kwe-INH, okukhombisa ukungabi nakuphikwa kwe-INH. |
| Izinsimbi ezisebenzayo | SLAN-96P real-time PCRS SystemsI-Biorad CFX96 Systems real-time PCRI-Lightcycler480®Uhlelo lwe-PCR lwesikhathi sangempela |
Ukugeleza komsebenzi
Uma usebenzisa i-Macro & Micro-Test General DNA / RNA KIT (HWTS-3019) (engasetshenziswa nge-Macro & Micro-3006 condractor (HWS-3006c, i-HWS-3006b) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd. Ukukhishwa, engeza i-200μL yokulawulwa okubi futhi kusetshenzwe isampula ye-Sputum ukuthi ihlolwe Ngokulandelana, bese wengeza i-10μL yokulawulwa kwangaphakathi ngokwehlukana kulawulo olubi, kusetshenzwe isampula ye-sputum, futhi izinyathelo ezalandela kufanele zenziwe ngokuqondile ngokuya ngemiyalo yokukhishwa. Ivolumu yesampula ekhishwe i-200μL, futhi ivolumu yokuqedwa enconyiwe ingu-100μL.
