15-Type HR-HPV mRNA Detection - Ihlonza Ubukhona NOMSEBENZI we-HR-HPV

Umdlavuza womlomo wesibeletho, okuyimbangela ehamba phambili yokufa kwabantu besifazane emhlabeni wonke, ubangelwa ikakhulukazi ukutheleleka nge-HPV. Amandla e-oncogenic okutheleleka kwe-HR-HPV ancike ekukhulumeni okukhulayo kwezakhi zofuzo ze-E6 ne-E7. Amaprotheni e-E6 kanye ne-E7 abophezela kumaprotheni acindezela isimila i-p53 kanye ne-pRb ngokulandelana, futhi aqhubekisela phambili ukwanda nokuguqulwa kwamangqamuzana omlomo wesibeletho.

Kodwa-ke, ukuhlolwa kwe-HPV DNA kuqinisekisa ubukhona begciwane, akuhlukanisi phakathi kwezifo ezicashile nezibhalayo ngenkuthalo. Ngokuphambene, ukutholwa kokulotshiweyo kwe-HPV E6/E7 mRNA kusebenza njenge-biomarker ecacile kakhulu ye-viral oncogene expression, futhi ngaleyo ndlela, kuyisibikezelo esinembe kakhudlwana se-intraepithelial neoplasia engaphansi komlomo wesibeletho (CIN) noma i-invasive carcinoma.

I-HPV E6/E7 mRNAukuhlola kunikeza izinzuzo ezibalulekile ekuvimbeleni umdlavuza womlomo wesibeletho:

• Ukuhlolwa Kwengozi Okunembile: Ihlonza izifo ze-HPV ezisebenzayo, eziyingozi kakhulu, ihlinzeka ngokuhlolwa kwengozi okunembe kakhudlwana kunokuhlolwa kwe-HPV DNA.
• Isivivinyo Esisebenzayo: Iqondisa odokotela ekuhlonzeni iziguli ezidinga uphenyo olwengeziwe, kuncishiswe izinqubo ezingadingekile.
• Ithuluzi Lokuhlola Okungenzeka: Lingase lisebenze njengethuluzi lokuhlola elizimele ngokuzayo, ikakhulukazi kubantu abasengozini enkulu.
• Izinhlobo ezingu-15 ze-Human Papillomavirus E6/E7 I-Gene mRNA Detection Kit (i-Fluorescence PCR) evela ku-#MMT, ehlonza ngokufanelekile umaki wezifo ezingase zibe khona ze-HR-HPV, iyithuluzi eliwusizo lokuhlola i-HPV kanye/noma nokuphathwa kwesiguli.

Izici zomkhiqizo:

• Ukufakwa okuphelele: Izinhlobo eziyi-15 ze-HR-HPV ezihlobene nomdlavuza womlomo wesibeletho ezimboziwe;
• Ukuzwela okuhle kakhulu: amakhophi angu-500/mL;
• Ukucaciswa okuphezulu: Awukho umsebenzi wesiphambano nge-cytomegalovirus, i-HSV II kanye ne-DNA ye-genomic yomuntu;
• Okungabizi kakhulu: Kuhloswe ukuhlola okuhlobene eduze nesifo esingase sibe khona, ukuze kuncishiswe ukuhlolwa okungadingekile ngezindleko ezengeziwe;
• Ukunemba okuhle kakhulu: I-IC yayo yonke inqubo;
• Ukuhambisana okubanzi: Ngezinhlelo ze-PCR ezijwayelekile;