Ukutholwa kwe-mRNA ye-HR-HPV yohlobo lwe-15 - Kukhomba Ukuba khona kanye NOMSEBENZI we-HR-HPV

Umdlavuza wesibeletho, imbangela ehamba phambili yokufa kwabesifazane emhlabeni jikelele, ubangelwa kakhulu ukutheleleka kwe-HPV. Amandla okutheleleka kwe-HR-HPV okubangela umdlavuza ancike ekukhuleni kokubonakaliswa kwezakhi zofuzo ze-E6 ne-E7. Amaprotheni e-E6 ne-E7 abopha amaprotheni okucindezela isimila i-p53 ne-pRb ngokulandelana, futhi aqhubekisele phambili ukwanda nokuguqulwa kwamaseli esibeletho.

Kodwa-ke, ukuhlolwa kwe-HPV DNA kuqinisekisa ukuba khona kwegciwane, akubonisi phakathi kokutheleleka okufihliwe kanye nokutheleleka okubhaliwe ngokusebenzayo. Ngokuphambene nalokho, ukutholakala kwe-HPV E6/E7 mRNA transcripts kusebenza njenge-biomarker ecacile yokubonakaliswa kwe-viral oncogene esebenzayo, ngakho-ke, kuyisibonakaliso esinembile se-cervical intraepithelial neoplasia (CIN) noma i-invasive carcinoma.

I-HPV E6/E7 mRNAUkuhlolwa kunikeza izinzuzo ezibalulekile ekuvimbeleni umdlavuza wesibeletho:

• Ukuhlolwa Kwengozi Okunembile: Kuhlonza ukutheleleka kwe-HPV okusebenzayo nokuyingozi kakhulu, okunikeza ukuhlolwa kwengozi okunembe kakhulu kunokuhlolwa kwe-DNA ye-HPV.
• I-Triage Ephumelelayo: Iqondisa odokotela ekuboneni iziguli ezidinga uphenyo olwengeziwe, inciphisa izinqubo ezingadingekile.
• Ithuluzi Lokuhlola Elingase Libe Khona: Lingasebenza njengethuluzi lokuhlola elizimele esikhathini esizayo, ikakhulukazi kubantu abasengozini enkulu.
• Izinhlobo ezingu-15 ze-Human Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR) evela ku-#MMT, ethola ngokwekhwalithi uphawu lokutheleleka okungenzeka kube okuqhubekayo kwe-HR-HPV, iyithuluzi eliwusizo lokuhlola i-HPV kanye/noma ukuphathwa kwesiguli.

Izici zomkhiqizo:

• Ukumbozwa okugcwele: Izinhlobo ezingu-15 ze-HR-HPV ezihlobene nomdlavuza wesibeletho zimboziwe;
• Ukuzwela okuhle kakhulu: amakhophi angu-500/mL;
• Ukucaca okuphezulu: Akukho msebenzi ohlangene ne-cytomegalovirus, i-HSV II kanye ne-DNA ye-genomic yomuntu;
• Okonga izindleko: Izinhloso zokuhlola zihlobene kakhulu nesifo esingaba khona, ukuze kuncishiswe ukuhlolwa okungadingekile ngezindleko ezengeziwe;
• Ukunemba okuhle kakhulu: IC yenqubo yonke;
• Ukuhambisana okubanzi: Ngezinhlelo ze-PCR ezijwayelekile;