Ukutholwa kwe-BCR-ABL Okubalulekile Kokwelashwa kwe-CML

I-myelogenousleukemia engapheli (CML) yisifo esiyingozi samaseli e-hematopoietic stem. Ngaphezu kuka-95% weziguli ze-CML ziphethe i-Philadelphia chromosome (Ph) kumaseli azo egazi. Futhi isakhi sofuzo esihlanganisiwe se-BCR-ABL sakhiwe ukudluliselwa phakathi kwe-ABL proto-oncogene (Abelson murine leukemia viral oncogene homolog 1) engalweni ende ye-chromosome 9 (9q34) kanye nofuzo lwe-breakpoint cluster region (BCR) engalweni ende ye-chromosome 212 (22q12). Iphrotheni yayo ehlanganisiwe efakwe yileli jini inomsebenzi we-tyrosine kinase (TK), futhi yenza kusebenze izindlela zayo zokusayina ezansi nomfula (ezifana ne-RAS, PI3K, ne-JAK/STAT) ukuze kuthuthukiswe ukuhlukana kwamaseli futhi kuvimbele i-apoptosis yeseli, okwenza amangqamuzana andle kabi, futhi ngaleyo ndlela abangele ukuvela kwe-CML.

I-BCR-ABL fusion gene, ebalulekile ekuthuthukisweni kwe-CML, isebenza njengophawu olubalulekile lokuxilonga kwe CML. Ukutholwa kwayo kusiza ukuqapha isiguli's impendulo kuma-tyrosine kinase inhibitors (TKIs) futhi ibikezela ukuphindaphinda kwe-leukemia, okubalulekile ekulawuleni isiguli se-CML ngempumelelo.

I-BCR-ABL yomuntuIkhithi yokutholwa kwe-Fusion Gene Mutationkusuka ku-Macro & Micro-Test ukuze kutholwe ikhwalithi ye-BCR-ABL ezigulini ze-CML, isiza ukuxilongwa kwe-CML futhi iqondise ukwelashwa okuhlosiwe komtholampilo ukuthuthukisa amasu okwelapha okuphatha ngempumelelo isiguli.BCR-ABL fusion gene, okuyikuyintokubalulekileekuthuthukisweni kwe-CML,isebenza njengomaka okhiye wokuxilongaofCML. Ukutholwa kwayo kuyasiza ukuqaphaisiguli'simpendulo kuma-tyrosine kinase inhibitors (TKIs)futhiibikezela ukuphindaphinda kwe-leukemia, okusho ukuthikubalulekileto ngempumeleloCMLukuphathwa kwesiguli.

Ikhithi Yokuthola I-BCR-ABL Fusion Gene Mutation kusuka ku-Macro & Micro-Test ukuze kutholwe ikhwalithi ye-BCR-ABL ezigulini ze-CML, isizaCMLukuxilongwafuthiabaqondisiukwelashwa okuhlosiwe komtholampilotothuthukisaeamasu okwelapha okuphatha isiguli ngempumelelo.

OkupheleleDetection:Kanyekanyeukutholwaokwe3izakhi zofuzo ezivamile (P210, P190, P230)okweukusabalala okubanzi kanyeukuvimbela emacaleni aphuthelwe;

Ukuzwela Okuphezulu:Inguqulo ye-liquid ithola izakhi zofuzo ezixubilenge LoDamakhophi angu-1000/mL,futhi inguqulo ye-lyophilizedof 500 amakhophi/mL;

Kuhle kakhuluAukunemba: Iokwangaphakathiukulawulakanye ne-UNG enzyme ukuvimbela izinto ezingamanga&okungalungile;

Ukusebenza Okulula nokusebenza kahle:Ukusebenza kweshubhu elivaliwe,mahhala kwemikhiqizongemuva kokucubungula,futhiukutholwangaphakathiImizuzu engama-60okweinhloso kanye nemiphumela ethembekile;

OkuningiIzinketho:Izinguqulo eziwuketshezi kanye ne-lyophilized zezinketho zamaklayenti;

Ukuhambisana Okubanzi:Ihambisana nezinhlelo eziningi ze-PCR zokufinyeleleka kwelebhu ebanzi.