Ukuhlolwa kwe-HPV Genotyping njengezimpawu zokuxilonga zengozi yomdlavuza wesibeletho - Ngokusetshenziswa kokutholwa kwe-HPV Genotyping

Ukutheleleka nge-HPV kuvame kubantu abahlanganyela ocansini, kodwa ukutheleleka okuqhubekayo kwenzeka kuphela engxenyeni encane yamacala. Ukuqhubeka kwe-HPV kuhilela ingozi yokuthola izilonda zomlomo wesibeletho ngaphambi komdlavuza, futhi ekugcineni, umdlavuza womlomo wesibeletho.

Ama-HPV awakwazi ukukhuliswangaphakathi kwe-vitrongezindlela ezivamile, kanye nokwehlukahlukana kwemvelo okubanzi kwempendulo yomzimba e-humoral ngemva kokutheleleka kuphazamisa ukusetshenziswa kokuhlolwa kwe-antibody ethize ye-HPV ekuxilongweni. Ngakho-ke, ukuxilongwa kokutheleleka kwe-HPV kufezwa ngokuhlolwa kwama-molecule, ikakhulukazi ngokutholwa kwe-genomic HPV DNA.

Njengamanje, kunezindlela eziningi zokukhiqiza i-HPV ezithengiswayo. Ukukhethwa kwezindlela ezifanele kakhulu kuncike ekusetshenzisweni okuhlosiwe, okungukuthi: i-epidemiology, ukuhlolwa komjovo, noma izifundo zezokwelapha.

Ezifundweni ze-epidemiological, izindlela zokuhlola i-HPV genotyping zivumela ukutholwa kokwanda kohlobo oluthile lwesifo.
Ukuze kuhlolwe umuthi wokugoma, lokhu kuhlolwa kunikeza idatha mayelana nezinguquko ekusabalaleni kwezinhlobo ze-HPV ezingafakwanga emithini yokugoma yamanje, futhi kusiza ukulandelwa kwezifo eziqhubekayo.
Ezifundweni zezokwelapha, iziqondiso zamazwe ngamazwe zamanje zincoma ukusetshenziswa kwezivivinyo ze-HPV genotyping phakathi kwabesifazane abaneminyaka engama-30 nangaphezulu abane-cytology engemihle kanye nemiphumela emihle ye-HR HPV, ku-HPV-16 ekhethekile kanye ne-HPV-18. Ukuthola i-HPV nokuhlukanisa izinhlobo ze-genotype ezinobungozi obuphezulu kanye nobephansi kabili noma ngaphezulu ukuthola iziguli ezinezifo ezifanayo eziqhubekayo ze-genotype, okuholela ekuphathweni okungcono kwezokwelapha.

Amakhithi okuhlola ufuzo lwe-HPV lwe-Macro kanye ne-Micro-Test:

• Izinhlobo ezingu-14 ze-HPV (Uhlobo Lofuzo) Ikhithi Yokuthola I-Nucleic Acid (i-Fluorescence PCR)
• Ikhithi Yokuthola I-Nucleic Acid Eyomisiwe Ngokuqandisa Eyi-14 (Uhlobo Lokuhlonza Izakhi Zofuzo) (i-PCR Yokukhanya Kwe-Fluorescence)
• Ikhithi Yokuthola Izinhlobo ze-HPV ezingu-28 (i-Genotyping) (i-Fluorescence PCR)(ama-HR-HPV angu-18 + ama-LR-HPV angu-10)
• Ikhithi Yokuthola Izinhlobo ze-HPV ezingu-28 (i-Genotyping) ezomiswe ngokuqandisa (i-Fluorescence PCR)

Izici ezibalulekile zomkhiqizo:

• Ukutholwa ngasikhathi sinye kwezinhlobo eziningi zezakhi zofuzo ekuphenduleni okukodwa;
• Isikhathi esifushane sokushintsha i-PCR ukuze kwenziwe izinqumo ezisheshayo zezokwelapha;
• Izinhlobo eziningi zamasampula (umchamo/i-swab) ukuze kuhlolwe ukutheleleka kwe-HPV ngendlela ekhululekile nefinyeleleka kalula;
• Ukulawula Kwangaphakathi Okubili kuvimbela imiphumela engamanga futhi kuqinisekisa ukuthembeka kokuhlolwa;
• Izinguqulo eziwuketshezi nezithambile zezinketho zamakhasimende;
• Ukuhambisana nezinhlelo eziningi ze-PCR ukuze kube lula ukuzivumelanisa nezimo zelebhu.