Ukuhlolwa kwe-HPV Genotyping njenge-Diagnostic Biomarkers ye-Cervical Cancer Risk - Ezicelweni Zokutholwa Kwe-HPV Genotyping

Ukutheleleka nge-HPV kuvame kubantu abaya ocansini, kodwa ukutheleleka okuqhubekayo kwenzeka kuphela engxenyeni encane yamacala. Ukuphikelela kwe-HPV kubandakanya ingozi yokuthola izilonda zomlomo wesibeletho futhi, ekugcineni, umdlavuza womlomo wesibeletho.

Ama-HPV awakwazi ukukhuliswaku-vitrongezindlela ezijwayelekile, kanye nokwehlukahlukana okubanzi kwemvelo kokusabela kwamasosha omzimba ahlekisayo ngemva kokutheleleka kukhinyabeza ukusetshenziswa kokuhlolwa kwe-antibody eqondene ne-HPV ekuxilongeni. Ngakho-ke, ukuhlonzwa kokutheleleka nge-HPV kutholwa ngokuhlolwa kwamangqamuzana, ikakhulukazi ngokutholwa kwe-genomic HPV DNA.

Njengamanje, kunezinhlobonhlobo zezindlela zokuhweba ze-HPV ze-genotyping ezikhona. Ukukhethwa okufanele kakhulu kuncike ekusetshenzisweni okuhlosiwe, okungukuthi: i-epidemiology, ukuhlolwa komgomo, noma izifundo zomtholampilo.

Ezifundweni ze-epidemiological, izindlela ze-HPV genotyping zivumela ukudweba kohlobo oluthile lokuvama.
Ukuze kuhlolwe umgomo, lezi zivivinyo zihlinzeka ngedatha ngokuphathelene nezinguquko zokuvama kwezinhlobo ze-HPV ezingafakiwe emithini yamanje, futhi zenza kube lula ukulandelelwa kwezifo ezibeleselayo.
Ezifundweni zomtholampilo, imihlahlandlela yamanje yamazwe ngamazwe incoma ukusetshenziswa kokuhlolwa kwe-HPV genotyping phakathi kwabesifazane abaneminyaka engu-30 nangaphezulu abane-negative cytology kanye nemiphumela emihle ye-HR HPV, ku-HPV-16 ne-HPV-18 ekhethekile. Ukuthola i-HPV nokubandlulula ama-genotypes anobungozi obukhulu kanye nobuncane kabili noma ngaphezulu ukuze uthole iziguli ezinezifo ezifanayo ze-genotype eziqhubekayo, okuholela ekuphathweni okungcono komtholampilo.

I-Macro & Micro-Test HPV genotyping kits:

• Izinhlobo eziyi-14 ze-HPV (Genotyping) I-Nucleic Acid Detection Kit (Fluorescence PCR)
• Izinhlobo eziyi-14 ze-HPV eziqandisiwe (Genotyping) I-Nucleic Acid Detection Kit(Fluorescence PCR)
• Izinhlobo ezingama-28 ze-HPV (Genotyping) Ikhithi Yokubona (Fluorescence PCR)(18 HR-HPVs +10 LR-HPVs)
• Izinhlobo ezingama-28 ze-HPV eziqandisiwe(Genotyping) Ikhithi Yokuthola (i-Fluorescence PCR)

Izici zomkhiqizo ezibalulekile:

• Ukutholwa ngesikhathi esisodwa kwama-genotypes amaningi ekuphenduleni okukodwa;
• Isikhathi esifushane sokuguqula i-PCR ngezinqumo zomtholampilo ezisheshayo;
• Izinhlobo eziningi zesampula (umchamo/i-swab) ukuze uthole ukuhlolwa kokutheleleka nge-HPV okunethezekile nokufinyeleleka kalula;
• Izilawuli Zangaphakathi Ezimbaxambili zivimbela amaphuzu angamanga futhi ziqinisekisa ukwethembeka kokuhlolwa;
• Izinguqulo eziwuketshezi kanye ne-lyophilized zezinketho zamaklayenti;
• Ukuhambisana nezinhlelo eziningi ze-PCR zokuzivumelanisa nezimo zelebhu.