1. Ukubaluleka kokuthola
I-Group B streptococcus (GBS) ivame ukuhlanganiswa esithweni sangasese sabesifazane kanye nase-rectum, okungaholela ekuthelelekeni kokuqala (GBS-EOS) ezinsaneni ezisanda kuzalwa ngokudluliselwa kusuka kumama kuya enganeni, futhi kuyimbangela eyinhloko ye-pneumonia yezingane ezisanda kuzalwa, i-meningitis, i-septicemia ngisho nokufa. Ngo-2021, i-Expert Consensus on Clinical Management of High-Risk Newborn with Early-onset Infection in the Same Room of Mothers and Infants of China Moternal and Child Health Association yaphakamisa ukuthi ukuhlolwa kwe-GBS amasonto angu-35-37 ngaphambi kokubeletha kanye nokuvimbela ama-antibiotic ngaphakathi kokubeletha (IAP) kwakuyizinyathelo ezisebenzayo zokuvimbela i-GBS-EOS ezinsaneni ezisanda kuzalwa.
2. Izinselele ezibhekene nezindlela zokuthola zamanje
Ukuqhuma kwe-membrane ngaphambi kwesikhathi (i-PROM) kubhekisela ekuqhekekeni kwe-membrane ngaphambi kokubeletha, okuyinkinga evamile esikhathini sokubeletha. Ukuqhuma kwe-membrane ngaphambi kwesikhathi Ngenxa yokuqhuma kwe-membrane, i-GBS esithweni sangasese sabesifazane abakhulelwe kungenzeka ukuthi isakazekele phezulu, okuholela ekuthelelekeni ngaphakathi kwesibeletho. Ingozi yokutheleleka ilingana ngqo nokukhula ngesikhathi sokuqhuma kwe-membrane (> 50% yabesifazane abakhulelwe bebeletha zingakapheli amahora angu-1-2 ngemuva kokuqhuma kwe-membrane, noma ngisho namahora angu-1-2).
Izindlela zokuthola ezikhona azikwazi ukuhlangabezana ngokugcwele nezidingo zesikhathi (< 1h), ukunemba kanye nokutholwa kwe-GBS ngesikhathi sokulethwa.
| Amathuluzi okuthola | Isiko lamagciwane | Isikhathi samasiko: 18-24hUma ukuhlolwa kokuzwela kwemithi: khulisa amahora angu-8-16 | Izinga lokutholakala elihle elingu-60%; ngesikhathi senqubo yokuthatha isampula, isengozini yokungenwa amagciwane afana ne-Enterococcus faecalis ezungeze isitho sangasese sowesifazane kanye ne-anus, okuholela emiphumeleni emibi/engalungile. |
| I-Immunochromatography | Isikhathi sokuthola: 15min. | Ukuzwela kuphansi, futhi kulula ukuphuthelwa ukubonwa, ikakhulukazi uma inani lamagciwane lincane, kunzima ukulibona, futhi iziqondiso azinconywa kakhulu. | |
| I-PCR | Isikhathi sokutholwa: 2-3h | Isikhathi sokuthola singaphezu kwamahora ama-2, futhi ithuluzi le-PCR lidinga ukuhlolwa ngamaqoqo, futhi akunakwenzeka ukulandela ukuhlolwa. |
3. Okuvelele komkhiqizo we-Macro & Micro-Test
Ukutholwa okusheshayo: Kusetshenziswa indlela yokukhulisa izinga lokushisa njalo ye-enzyme enelungelo lobunikazi, iziguli ezine-HIV zingawazi umphumela ngokushesha nje kwemizuzu emi-5.
Ukutholwa nganoma yisiphi isikhathi, asikho isidingo sokulinda: ifakwe i-nucleic acid amplification analyzer engaguquki yokushisa i-Easy Amp, futhi amamojula amane asebenza ngokuzimela, futhi amasampula ahlolwa njengoba efika, ngakho asikho isidingo sokulinda amasampula.
Uhlobo lwesampula eziningi: i-vaginal swab, i-rectal swab noma i-mixed vaginal swab ingatholakala, ehlangabezana nezincomo zemihlahlandlela ye-GBS, ithuthukise izinga lokutholakala elihle futhi yehlise izinga lokuhlonza okungalungile.
Ukusebenza okuhle kakhulu: ukuqinisekiswa kwesampula enkulu yezokwelapha okunama-centre amaningi (> amacala ayi-1000), ukuzwela okungu-100%, ukucaciswa okungu-100%.
I-reagent evulekile: iyahambisana nethuluzi le-PCR eliyi-fluorescence elijwayelekile lamanje.
4. Ulwazi lomkhiqizo
| Inombolo Yomkhiqizo | Igama Lomkhiqizo | Imininingwane | Inombolo yesitifiketi sokubhalisa |
| I-HWTS-UR033C | Ikhithi yokuthola i-Streptococcus nucleic acid yeqembu B (Ukukhulisa i-Enzymatic Probe Isothermal) | Ukuhlolwa okungu-50/ikhithi | Ukubhaliswa Kwemishini YaseShayina 20243400248 |
| I-HWTS-EQ008 | Uhlelo Lokuthola Isothermal Lwe-Easy Amp Real-time Fluorescence | I-HWTS-1600P (iziteshi ezi-4) | Ukubhaliswa Kwemishini YaseShayina 20233222059 |
| I-HWTS-1600S (iziteshi ezimbili) | |||
| I-HWTS-EQ009 |
Isikhathi sokuthunyelwe: Mashi-07-2024