Iqembu B Streptococcus nucleic acid ukuthola ikhithi(Enzymatic Probe Isothermal Amplification)
1.Ukubaluleka kokutholwa
I-Group B streptococcus (GBS) ivamise ukugqunywa esithweni sangasese sabesifazane kanye ne-rectum, okungaholela ekuthelelekeni kwangaphambi kwesikhathi (GBS-EOS) ezinsaneni ezisanda kuzalwa ngokuthelelana okuqondile kusuka kumama kuya enganeni, futhi iyimbangela eyinhloko yenyumoniya ezisanda kuzalwa, i-meningitis, i-septicemia kanye ngisho nokufa.Ngo-2021, I-Expert Consensus on Clinical Management yezingane ezisanda kuzalwa ezisengcupheni enkulu ezinokutheleleka kwangaphambi kwesikhathi egumbini elifanayo lomama nezinsana ze-China Maternal and Child Health Association yaphakamisa ukuthi ukuhlolwa kwe-GBS emasontweni angama-35-37 ngaphambi kokubeletha kanye nokuvimbela i-intrapartum antibiotic (IAP). ) kwakuyizinyathelo eziphumelelayo zokuvimbela i-GBS-EOS ezinganeni ezisanda kuzalwa.
2. Izinselele ezibhekene nezindlela zamanje zokubona
Ukuphuka ngaphambi kwesikhathi kolwelwesi (PROM) kubhekisela ekuqhekekeni kolwelwesi ngaphambi kokusikwa, okuyinkinga evamile esikhathini sokuzala.Ukuphuka ngaphambi kwesikhathi kolwelwesi Ngenxa yokwephuka kolwelwesi, i-GBS esithweni sangasese sabesifazane abakhulelwe maningi amathuba okuthi isabalale iye phezulu, okuholela ekuthelelekeni kwe-intrauterine.Ingozi yokutheleleka ilingana ngqo nokukhula ngesikhathi sokuqhekeka kolwelwesi (> 50% wabesifazane abakhulelwe bebeletha phakathi namahora angu-1-2 ngemva kokugqabuka kolwelwesi, noma amahora angu-1-2).
Izindlela ezikhona zokuthola azikwazi ukuhlangabezana ngokugcwele nezimfuneko zokufika ngesikhathi (< 1h), ukunemba kanye nokutholwa kwe-GBS ocingweni ngesikhathi sokulethwa.
| Amathuluzi okuthola | Amasiko amagciwane | Isikhathi Sesiko : 18-24hUma ukuhlolwa kokuzwela kwezidakamizwa : ukwanda kwe-8-16h | 60 % izinga lokutholwa okuhle;phakathi nenqubo yokuthatha amasampula, ingangenwa amagciwane afana ne-Enterococcus faecalis azungeze isitho sangasese sowesifazane kanye nedidi, okuholela emiphumeleni engemihle/engelona iqiniso. |
| I-Immunochromatography | Isikhathi sokutholwa: 15min. | Ukuzwela kuphansi, futhi kulula ukuphuthelwa ukubonwa, ikakhulukazi uma inani lamagciwane lilincane, kunzima ukulibona, futhi imihlahlandlela ayinconywa kancane. | |
| I-PCR | Isikhathi sokutholwa: 2-3h | Isikhathi sokutholwa singaphezu kwamahora angu-2, futhi ithuluzi le-PCR lidinga ukuhlolwa ngamaqoqo, futhi akwenzeki ukulandela ukuhlolwa. |
3. Izithombe ezinhle kakhulu zomkhiqizo we-Macro & Micro-Test
Ukutholwa ngokushesha: Ngokusebenzisa indlela yokugaya i-enzyme enelungelo lobunikazi yokuhlola izinga lokushisa elingaguquki, iziguli ezine-positive zingakwazi ukwazi umphumela ngokushesha ngemva kwemizuzu emi-5.
Ukutholwa nganoma yisiphi isikhathi, asikho isidingo sokulinda: ifakwe i-nucleic acid amplification analyzer ehlala njalo, futhi amamojula amane asebenza ngokuzimela, futhi amasampula ahlolwe njengoba efika, ngakho-ke asikho isidingo sokulinda amasampula.
Uhlobo lwamasampula amaningi: i-swab ye-vaginal, i-rectal swab noma i-swab ye-vaginal exubile ingatholwa, ehlangabezana nokunconywa kwemihlahlandlela ye-GBS, ithuthukisa izinga lokutholwa okuhle futhi yehlise izinga lokungaxilongwa kahle.
Ukusebenza okuhle kakhulu: ukuqinisekiswa komtholampilo okuyizikhungo eziningi ezinkulu (> amacala ayi-1000), ukuzwela 100%, ukucaciswa okungu-100%.
I-reagent evuliwe: ihambisana nethuluzi lamanje le-PCR le-fluorescence quantitative.
4. Ulwazi lomkhiqizo
| Inombolo Yomkhiqizo | Igama Lomkhiqizo | Ukucaciswa | Inombolo yesitifiketi sokubhalisa |
| I-HWTS-UR033C | Iqembu B Streptococcus nucleic acid ukuthola ikhithi (I-Enzymatic Probe Isothermal Amplification) | 50 ukuhlolwa/ikhithi | China Machinery Registration 20243400248 |
| I-HWTS-EQ008 | I-Easy Amp Real-time Fluorescence Isothermal Detection System | HWTS-1600P (4-channel) | China Machinery Registration 20233222059 |
| HWTS-1600S (2-channel) | |||
| I-HWTS-EQ009 |
Isikhathi sokuthumela: Mar-07-2024