Ukuguquka kwamaphuzu esakhini se-KRAS kuhilelekile ezinhlobonhlobo zezimila zabantu, ngamazinga okuguquka angaba ngu-17%–25% kuzo zonke izinhlobo zezimila, 15%–30% kumdlavuza wamaphaphu, kanye no-20%–50% kumdlavuza we-colorectal. Lokhu kuguquka kuqhuba ukumelana nokwelashwa kanye nokuqhubekela phambili kwesimila ngendlela ebalulekile: iphrotheni ye-P21 ebhalwe yi-KRAS isebenza ngaphansi kwendlela yokubonisana ye-EGFR. Uma i-KRAS isishintshile, ihlala ivuselela ukuhanjiswa kwesignali, okwenza ukwelashwa okuqondiswe ku-EGFR okungaphezulu kungasebenzi futhi kuholele ekwandeni okuqhubekayo kwamaseli abulalayo. Ngenxa yalokho, ukuguquka kwe-KRAS kuhlotshaniswa nokumelana nama-inhibitor e-EGFR tyrosine kinase kumdlavuza wamaphaphu kanye nokwelashwa kwama-antibody okulwa ne-EGFR kumdlavuza we-colorectal.

Ngo-2008, i-National Comprehensive Cancer Network (NCCN) yasungula iziqondiso zemitholampilo ezincoma ukuhlolwa kokuguquka kwe-KRAS kuzo zonke iziguli ezinomdlavuza we-colorectal osakazekayo (mCRC) ngaphambi kokwelashwa. Iziqondiso ziqokomisa ukuthi iningi lokuguqulwa kwe-KRAS okusebenza kwenzeka kuma-codon 12 no-13 e-exon 2. Ngakho-ke, ukutholakala kokuguqulwa kwe-KRAS okusheshayo nokunembile kubalulekile ekuqondiseni ukwelashwa okufanele kwezokwelapha.

Kungani Ukuhlolwa kwe-KRAS Kubalulekile Ku-Mi-estastaticCi-olorectalCi-ancer(mCRC)

Umdlavuza we-Colorectal (CRC) awusona isifo esisodwa kodwa iqoqo lezinhlobo ezihlukile zama-molecule. Ukuguqulwa kwe-KRAS—okukhona cishe ku-40-45% weziguli ze-CRC—kusebenza njengokushintshana okuqhubekayo, okukhuthaza ukukhula komdlavuza ngaphandle kwezimpawu zangaphandle. Ezigulini ezine-mCRC, isimo se-KRAS sinquma ukusebenza kahle kwama-antibody e-anti-EGFR monoclonal njenge-Cetuximab ne-Panitumumab:

I-KRAS Yohlobo Lwendle:Iziguli cishe zizozuza ekwelashweni okulwa ne-EGFR.

I-KRAS Eguquliwe:Iziguli azizuzi lutho kulezi zidakamizwa, zibeka engcupheni imiphumela emibi engadingekile, izindleko ezengeziwe, kanye nokubambezeleka kokwelashwa okusebenzayo.

Ngakho-ke ukuhlolwa kwe-KRAS okunembile nokubucayi kuyisisekelo sokuhlela ukwelashwa komuntu siqu.

Inselele Yokuthola: Ukuhlukanisa Isignali Yokuguquka

Izindlela zendabuko zivame ukungabi nokuzwela kokuguqulwa kwezakhi zofuzo okunobuningi obuncane, ikakhulukazi kumasampula anokuqukethwe okuphansi kwesimila noma ngemva kokususwa kwe-calcium. Ubunzima busekuhlukaniseni isignali ye-DNA eguquliwe engabonakali ngokumelene nesizinda se-wild-type esiphezulu—njengokuthola inalithi esigxotsheni sotshani. Imiphumela engalungile ingaholela ekwelashweni okungelona iqiniso kanye nemiphumela ebucayi.

Isixazululo Sethu: Kuklanywe Ngokunembile Ukuze Kutholakale Ukuguqulwa Okuqinisekile

Ikhithi yethu yokuthola ukuguquka kwe-KRAS ihlanganisa ubuchwepheshe obuthuthukisiwe ukuze kunqotshwe lokhu kulinganiselwa, okuletha ukunemba okumangalisayo kanye nokuthembeka kwesiqondiso sokwelashwa kwe-mCRC.

Indlela Ubuchwepheshe Bethu Obuqinisekisa Ngayo Ukusebenza Okuphezulu

• Ubuchwepheshe be-ARMS obuthuthukisiwe (Uhlelo Lokuguqula Ukuguquguquka Kwe-Amplification Refractory): Busekelwe kubuchwepheshe be-ARMS, bufaka ubuchwepheshe bokuthuthukisa obungobomuntu siqu ukuze kwandiswe ukucaciswa kokutholwa.
• Ukucebisa nge-Enzymatic: Kusebenzisa ama-endonuclease okukhawulela ukugaya iningi lesizinda se-genome yomuntu sasendle, kugcinwe izinhlobo eziguquliwe, ngaleyo ndlela kuthuthukiswe ukuxazululwa kokutholwa futhi kuncishiswe ukwanda okungaqondile ngenxa yesizinda se-genomic esiphezulu.
• Ukuvimba Izinga Lokushisa: Kwethula izinyathelo ezithile zokushisa enqubweni ye-PCR, okubangela ukungalingani phakathi kwama-primer aguquliwe namathempulethi e-wild-type, ngaleyo ndlela kunciphisa isizinda se-wild-type futhi kuthuthukise ukuxazululwa kokutholwa.
• Ukuzwela Okuphezulu: Ithola ngokunembile i-DNA eguquliwe engaphansi kwe-1%.
• Ukunemba Okuhle Kakhulu: Isebenzisa amazinga angaphakathi kanye ne-enzyme ye-UNG ukuvimbela imiphumela emihle nemibi engamanga.
• Kulula Futhi Kuyashesha: Kuqeda ukuhlola cishe ngemizuzu eyi-120, kusetshenziswa amashubhu amabili okusabela ukuze kube lula ukuthola izinguquko eziyisishiyagalombili ezihlukile, okuveza imiphumela eqondile nethembekile.
• Ukuhambisana Kwethuluzi: Kuvumelana nezinsimbi ezahlukahlukene ze-PCR.

Umuthi onembile kumdlavuza we-colorectal uqala ngokuxilongwa okunembile kwama-molecule. Ngokusebenzisa i-KRAS Mutation Detection Kit yethu, ilabhorethri yakho ingaletha imiphumela eqinisekile, esebenzisekayo ebumba ngqo indlela yokwelashwa kwesiguli.

Nika amandla ilebhu yakho ngobuchwepheshe obuthembekile nobusezingeni eliphezulu—futhi unike amandla ukunakekelwa komuntu siqu ngempela.

Xhumana nathi: ukumaketha@mmtest.Com

Funda kabanzi mayelana nokuhlanganisa lesi sixazululo esithuthukisiwe emsebenzini wakho wokuxilonga.

#Umdlavuza #I-DNA #Ukuguquka #Ukunemba #Okuqondiwe #Ukwelashwa #Umdlavuza

Ukuvula Umuthi Oqondile Kumdlavuza We-Colorectal

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