Ucwaningo lwamuva nje lwe-metagenomic luveze izinhlobo eziningi zamagciwane namabhaktheriya athwalwa amagundane eSifundazweni saseHeilongjiang, okuhlanganisa igciwane iLassa, igciwane i-Amur, i-Salmonella, kanye ne-Yersinia pestis.
Ucwaningo lusebenziseIkhithi Yokucebisa I-RNA Ye-Pathogen evela ku-Macro & Micro-Test, ngokusekelwe kubuchwepheshe bokubamba i-probe. Ngokususa ngokukhetha i-host rRNA kanye nezinye izingcezu zomsingathi ezigciniwe, le ndlela igcine ngempumelelo i-RNA yamagciwane, yathuthukisa kakhulu ukuzwela kokutholwa kwe-pathogen. Lesi sinyathelo sokucebisa i-RNA ye-pathogen sasibalulekile ekufinyeleleni izinga eliphezulu lokutholwa futhi sanikeza ukwesekwa okuqinile kobuchwepheshe bokuqapha i-pathogen.
Amagundane: Izindawo Eziyinhloko Zokugcina Izifo Ezibangelwa Yizilwane Ezifuywayo
Amagundane ayiqembu lezilwane ezincelisayo ezicebile kakhulu futhi ezisakazeke kabanzi, eliqukethe izinhlobo ezingaphezu kuka-2,000 emhlabeni wonke futhi liqukethe ngaphezu kuka-40% wazo zonke izinhlobo zezilwane ezincelisayo.
Ngaphandle kokubaluleka kwazo kwezemvelo, amagundane asebenza njengezindawo zokugcina ezinkulu kanye nezindawo zokuthwala izifo eziningi ezibangelwa yizilwane.Ngenxa yokuthi izindawo zokuhlala zamagundane zivame ukuhlangana nezindawo zabantu—ngokulunywa ngqo, ukuthintana nendle, noma ukudluliselwa okungaqondile ngama-fleas nezibungu—amagciwane abawagcinayo ayingozi eqhubekayo empilweni yomphakathi.
Okuphawulekayo ukuthi izifo ezithelelanayo ezingaphezu kuka-70% zivela ezilwaneni zasendle, kanti amagundane amelela enye yezindawo ezibaluleke kakhulu zokugcina amagciwane.
Isifunda Somngcele saseHeilongjiang: Umugqa Ongaphambili Wokuqapha Amagciwane
I-Heilongjiang, isifundazwe esisemngceleni enyakatho-mpumalanga yeShayina, inezinsiza eziningi zemvelo futhi ihlanganyela imingcele namazwe amaningi. Ukuhlanganiswa kokuhlukahluka okucebile kwezinhlobo zezinto eziphilayo kanye nokuhamba njalo kwabantu benqamula imingcele kudala izimo ezinhle zokusabalala kwezifo.
Phakathi kukaJuni noJulayi 2021, abacwaningi baqoqa amagundane asendle angu-67 eHengdaohezi Town, eMudanjiang City. Amasampula afaka:
l 20I-Apodemus peninsulae
l 47I-Myodes rufocanus
Zonke izinhlobo zathathwa ezindaweni zokuhlala nasemapulazini azungezile, okugqamisa ukuxhumana okuseduze phakathi kwezindawo zokuhlala zabantu kanye nezindawo zokuhlala zezilwane zasendle.
Ubuchwepheshe Bokucebisa I-Pathogen: Ukunqoba Ukuphazamiseka Kwesizinda Sombungazi
Ekulandeleni kwe-metagenomic, i-RNA yomnikazi ivame ukubusa ukufundwa kokulandelana, ifihla izimpawu ze-pathogen ezinobuningi obuphansi.
Ukuze kuxazululwe le nselele, ucwaningo lusebenziseIkhithi Yokuthola I-Metagenomic Ye-Macro & Micro-Test. Kusetshenziswa ubuchwepheshe bokubamba i-probe, ama-probe aklanywe ngokukhethekile abopha i-rRNA yokusingatha futhi alondoloze ukulandelana kwawo, okwenza kube lula ukuncipha kwawo futhi ngaleyo ndlela acebise i-RNA yamagciwane.
Le ndlela ithuthukise kakhulu ukuzwela kokutholwa kwezifo, okuvumela ukulandelana kwezansi ukuthi kutholakale ingxenye ephezulu yokufundwa kwezifo. Isinyathelo sokucebisa sabeka isisekelo esiqinile sokuchaza ngokunembile ububanzi bezifo obuthwalwa amagundane.
Ngemva kokunongwa, amasampula e-RNA ahlolwe ngokuphambene, akhiwa emtatsheni wezincwadi, kanye nokulandelana kwemiphumela ephezulu. Ukuhlaziywa kwe-Bioinformatic kufake:
l Ukuhlunga i-genome ye-Host
Ukuhlukaniswa kwe-taxonomic kusetshenziswa i-Kraken2
l Ukulungiswa kobuningi nge-Bracken
Lolu hlelo lokusebenza ludale iphrofayili ephelele yemiphakathi yamagciwane kanye namagciwane.
Okutholakele Ocwaningweni: Ukusabalala Kwamagciwane Amaningi Namabhaktheriya Ngasikhathi Sinye
Okutholakele Okubangelwa Yigciwane
Amagciwane atholakele ayengawemindeni engama-21 egciwane, okuhlanganisaI-ArenaviridaefuthiI-Hantaviridae.
Ngokudlulade novoukuhlanganiswa kusetshenziswa iMegahit, kwakhiqizwa izifo ezingamagciwane ezingu-154, ezingu-110 zazo ezazihambisana namagciwane angu-19 avela emindenini eyisishiyagalombili.
In I-Apodemus peninsulae, kwatholakala amagciwane alandelayo:
l Amagciwane e-Arena: igciwane leLassa, igciwane le-Wenzhou, igciwane leGairo
l Ama-Hantavirus: igciwane le-Amur, igciwane le-Puumala
In I-Myodes rufocanus, i-rat arterivirus kanye ne-Amur virus kutholakale.
Phakathi kwalezi zifo ezibangelwa amagciwane:
Igciwane le-Lassa liyimbangela yomkhuhlane wegazi obangelwa yigciwane, kanti amazinga okufa kwabantu afinyelela cishe ku-20%.
Igciwane le-Amur lingenye yezifo ezibangela umkhuhlane wegazi ohambisana nesifo sezinso (i-HFRS). I-China ibika umthwalo omkhulu wokutheleleka kwe-hantavirus emhlabeni jikelele.
Okutholakele Kwamagciwane
Izakhi zamagciwane eziyi-1,051 sezizonke zachazwa.
Izinhlobo eziningi kakhulu yilezi:
lI-Salmonella(26–31%)
lI-Yersinia(11–23%)
I-Salmonellaiyigciwane elikhulu elitholakala ekudleni, elibangela amacala alinganiselwa ezigidini ezingu-9 omkhuhlane we-typhoid kanye nokufa kwabantu ababalelwa ku-110,000 minyaka yonke emhlabeni jikelele.
I-Yersinia pestis, imbangela yesifo esiwumshayabhuqe, ibilokhu ibangela izifo ezintathu zobhubhane zomlando. Isifundazwe saseHeilongjiang saziwa njengenye yezindawo zemvelo ezigxile kuzo izifo eziwumshayabhuqe eShayina futhi sihlala siyisifunda esibaluleke kakhulu sokuqapha isifo esiwumshayabhuqe.
Ukubaluleka Kwalolu Cwaningo
Iminikelo Yesayensi
Lolu cwaningo lumelela umbiko wokuqala wezingcezu zegciwane i-Lassa kanye negciwane le-arteritis yamagundane kumagundane aseHeilongjiang, okwandisa isizindalwazi se-pathogen esithwalwa amagundane saseShayina.
Ichaza ngokuhlelekile imiphakathi yamagciwane kanye namagciwane ezindaweni zamagundane endawo, ihlinzeka ngemininingwane eyisisekelo yokuqapha izifo zezilwane ezindaweni ezisemngceleni.
Imiphumela Yezempilo Yomphakathi
Okutholakele kugcizelela isidingo sokuqapha okuqhubekayo kanye nokuhlolwa kwezifoA. peninsulaefuthiM. rufocanus.
Idatha inikeza ubufakazi besayensi obusekela amasu okulawula umthombo wezifo ezifana nobhubhane kanye nomkhuhlane wegazi ohambisana nesifo sezinso.
Amandla Ezindlela Zokusebenza
Ukulandelana kwe-metagenomic kwenza kube lula ukuthola izifo ezithelelanayo ezaziwayo nezintsha.
Kulolu cwaningo, ubuchwepheshe bokucebisa amagciwane be-Macro & Micro-Test bubonise ukusebenza okuqinile, buthuthukisa ngempumelelo ukutholakala kwamagciwane aqondiwe kumasampula ezinto eziphilayo eziyinkimbinkimbi. Imiphumela iqinisekisa ikhono layo lokusekela ukuhlonza amagciwane azwela kakhulu ekuqapheni izilwane zasendle.
Izincomo Zomkhiqizo
Ngokusekelwe ekusetshenzisweni kwayo okuphumelelayo kulolu cwaningo, i-Macro & Micro-Test Pathogen RNA Enrichment Kit inconywa kakhulu ocwaningweni lokuqapha i-metagenomic.
Ngokuqeda ngempumelelo ama-nucleic acid e-host kanye nokuthuthukisa ukulandelana kwamagciwane, le khithi ithuthukisa kakhulu ukuzwela kokutholwa kanye nekhwalithi yedatha. Imelela ithuluzi elibalulekile lokuthola izifo ezinembile kumasampula ayinkimbinkimbi futhi inikeza ukwesekwa okuqinile kobuchwepheshe bokuqapha impilo yomphakathi, ukuhlolwa kwengozi ye-zoonotic, kanye nokulungiselela ukuqubuka kwesifo.
Contact to learn more: marketing@mmtest.com
Isikhathi sokuthunyelwe: Mashi-02-2026