Orientia tsutsugamushi
Igama lomkhiqizo
I-HWTS-OT002B Orientia tsutsugamushiI-Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Scrub typhus yisifo se-acute febrile esibangelwa ukutheleleka kwe-Orientia tsutsugamushi (Ot). I-Orientia scrub typhus iyi-Gram-negative obligate intracellular parasitic microorganism. I-Orientia scrub typhus iyingxenye yohlobo lwe-Orientia ngokulandelana kwe-Rickettsiales, umndeni wakwa-Rickettsiaceae, kanye nohlobo lwe-Orientia. I-Scrub typhus isakazwa ngokulunywa yizibungu ze-chigger ezithwala amagciwane. Emtholampilo ibonakala ngemfiva ephezulu engazelelwe, i-eschar, i-lymphadenopathy, i-hepatosplenomegaly, ne-peripheral blood leukopenia, njll. Ezimweni ezimbi kakhulu, ingabangela i-meningitis, ukwehluleka kwesibindi nezinso, ukwehluleka kwesistimu yezitho eziningi, ngisho nokufa.
Isiteshi
| FAM | Orientia tsutsugamushi |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo lwesifanekiso | i-serum entsha |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Amakhophi/μL |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 ye-Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
I-reagent yokukhipha enconyiwe: I-Macro & Micro-TestOkujwayelekileI-DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhishwa kufanele kwenziwe ngokuvumelana nomyalelo wokusetshenziswa kwalesi sici esisebenzayo. Ivolumu yesampula ekhishiwe ingu-200µL, futhi ivolumu ye-elution enconyiwe ithi100µL.
