Orientia tsutsugamushi
Igama lomkhiqizo
I-HWTS-OT002B Orientia tsutsugamushiIkhithi Yokuthola I-Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
I-Scrub typhus yisifo esibangelwa umkhuhlane obangelwa ukutheleleka yi-Orientia tsutsugamushi (Ot). I-Orientia scrub typhus iyi-microorganism e-Gram-negative ebophayo ngaphakathi kweseli. I-Orientia scrub typhus ingeye-genus Orientia ngokulandelana kwe-Rickettsiales, umndeni we-Rickettsiaceae, kanye ne-genus Orientia. I-Scrub typhus idluliselwa kakhulu ngokulunywa yizibungu ze-chigger ezithwala amagciwane. Ibonakala ngokwezokwelapha ngomkhuhlane omkhulu ongazelelwe, i-eschar, i-lymphadenopathy, i-hepatosplenomegaly, kanye ne-peripheral blood leukopenia, njll. Ezimweni ezimbi kakhulu, ingabangela i-meningitis, ukwehluleka kwesibindi nezinso, ukwehluleka kwezitho eziningi zomzimba, ngisho nokufa.
Isiteshi
| I-FAM | Orientia tsutsugamushi |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | i-serum entsha |
| Tt | ≤38 |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-500/μL |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.) I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Uhlelo lwe-BioRad CFX96 Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 Real-Time PCR |
Ukugeleza Komsebenzi
I-reagent yokukhipha enconyiwe: I-Macro kanye ne-Micro-TestJikeleleIkhithi ye-DNA/RNA (HWTS-301)9) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhishwa kufanele kwenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhipha. Umthamo wesampula okhishwe ungama-200µL, futhi umthamo wokuhlanza onconywayo ungowe100µL.
