I-Orientia tsutsugamushi
Igama lomkhiqizo
I-HWTS-OT002B I-Orientia tsutsugamushiI-nucleic acid detection kit (i-fluorescence PCR)
I-Epidemiology
I-Scrub Tyhus yisifo esibuhlungu esibuhlungu esibangelwa ukutheleleka kwe-OrientieTia Tsutsugamushi (OT). I-Orientia Scrub tyhus iyi-gram-negative eduduza i-rasititic microorganism. I-Orientie Scrub Tyhus ingeyakwaGenus Orientias ku-Order Rickettsiales, umndeni ricktettsiaeae, kanye nohlobo lwe-Orientia. I-Scrub Tyhus idluliselwa ikakhulukazi ngokulunywa yizibungu ze-chigger zithwele ama-pathogen. Ibonakala ngomtholampilo ngomkhuhlane ophakeme ophakeme, e-Esychar, i-lymphadenopathy, i-hepatosplenomegalyy, kanye ne-peripheral igazi leukopenia, njll. Ezimweni ezinzima, kungadala ukwehluleka kwezimo zezinso, nokufa.
Insungubezi
| -Yenza | I-Orientia tsutsugamushi |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | i-serum entsha |
| Tt | ≤38 |
| CV | ≤5.0% |
| Indlu edayisa insimbi | Amakhophi angama-500 / μL |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real ISlan-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.) I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 kanye nezinhlelo zokutholwa kwe-PRCR yesikhathi sangempela (FQD-96A, Hangzhou Bioer Technology) I-MA-6000 Real-Time Cycler Cycler yesikhathi sangempela (Suzhou Molarray Co, Ltd.) I-Biorad CFX96 Uhlelo lwe-Real-Time PCR, Biorad CFX Opus 96 Uhlelo lwe-Real-Time PCR |
Ukugeleza komsebenzi
I-reaglent enconyiwe ye-realent: Macro & Micro-TestJwayelekileI-DNA / RNA KIT (HWTS-3019) (okungasetshenziswa nge-macro & micro-test automatic extomactor ye-nucleic acid extractor (HWS-EQ011)) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd. Ukukhishwa kufanele kwenziwe ngokuya ngokufundiswa okusetshenziselwa lokhu ukukhishwa kwe-realent. Ivolumu yesampula ekhishwe i-200μL, futhi ivolumu yokuqedwa elinconyiwe100μl.
