I-Plasmodium Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-OT033-Nucleic Acid esekelwe ku-Enzymatic Probe Isothermal Amplification (EPIA) ye-Plasmodium
Isitifiketi
CE
I-Epidemiology
Umalaleveva ubangelwa yiPlasmodium. I-Plasmodium iyi-eukaryote eneseli elilodwa, okuhlanganisa iPlasmodium falciparum, iPlasmodium vivax kanye nePlasmodium ovale. Yisifo esibangelwa yizinambuzane esidluliselwa yizimbotshana kanye negazi, okulimaza kakhulu impilo yabantu. Phakathi kwezinambuzane ezibangela umalaleveva kubantu, iPlasmodium falciparum iyona ebulala kakhulu. Isikhathi sokufukamela kwezinambuzane ezahlukene ze-malaria sihlukile. Esifushane kakhulu siyizinsuku eziyi-12 kuya kwezingama-30, kanti asebekhulile bangafinyelela cishe unyaka owodwa. Izimpawu ezifana nokubanda, umkhuhlane, kanye nomkhuhlane zingavela ngemva kokuqala komalaleveva, futhi i-anemia kanye ne-splenomegaly kungabonakala; izimpawu ezinzima ezifana ne-coma, i-anemia enzima, kanye nokwehluleka kwezinso okukhulu kungaholela ekufeni. Umalaleveva usakazeke emhlabeni wonke, ikakhulukazi ezindaweni ezishisayo nezishisayo njenge-Afrika, iMelika Ephakathi, kanye neNingizimu Melika.
Njengamanje, izindlela zokuthola zifaka phakathi ukuhlolwa kwegazi, ukutholwa kwe-antigen, kanye nokutholwa kwe-nucleic acid. Ukutholwa kwamanje kwe-Plasmodium nucleic acid ngokusebenzisa ubuchwepheshe bokukhulisa i-isothermal kunempendulo esheshayo kanye nokutholwa okulula, okufanelekelayo ukutholwa kwezindawo ezinkulu ezinesifo sikamalaleveva.
Isiteshi
| I-FAM | I-Plasmodium nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | igazi lonke |
| Tt | <30 |
| CV | ≤10.0% |
| I-LoD | Amakhophi ama-5/uL |
| Ukucaciswa | Akukho ukuhlangana kwegciwane le-H1N1 influenza, igciwane le-H3N2 influenza, igciwane le-influenza B, igciwane le-dengue fever, igciwane le-encephalitis laseJapan, igciwane le-respiratory syncytial, i-meningococcus, igciwane le-parainfluenza, i-rhinovirus, i-toxic dysentery, i-golden grape Cocci, i-Escherichia coli, i-Streptococcus pneumonia, i-Klebsiella pneumoniae, i-Salmonella typhi, i-Rickettsia tsutsugamushi |
| Izinsimbi Ezisebenzayo | Uhlelo Lokuthola Isothermal Lokukhanya Kwe-Easy Amp Real-time (HWTS1600) Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
