Uhlobo lwegciwane le-Poliovirus Ⅰ
Igama lomkhiqizo
I-HWTS-EV006- Ikhithi Yokuthola I-Poliovirus Uhlobo Ⅰ I-Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
I-Poliovirus yigciwane elibangela i-poliomyelitis, isifo esithathelwanayo esisakazeka kabanzi. Leli gciwane livame ukuhlasela uhlelo lwezinzwa oluphakathi, lilimaza amangqamuzana ezinzwa ezinyakazayo ophondweni olungaphambili lomgogodla, futhi libangele ukukhubazeka okuthambile kwezitho, okuvame kakhulu ezinganeni, ngakho-ke libizwa nangokuthi i-polio. Ama-poliovirus angaphansi kohlobo lwe-enterovirus lomndeni we-picornaviridae. I-Poliovirus ihlasela umzimba womuntu futhi isakazeka kakhulu ngomgudu wokugaya ukudla. Ingahlukaniswa ngama-serotype amathathu ngokuya ngokuvikeleka komzimba, uhlobo I, uhlobo II, kanye nohlobo III.
Isiteshi
| I-FAM | igciwane le-polio uhlobo I |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | Isampula yendle esanda kuqoqwa |
| Ct | ≤38 |
| CV | <5.0% |
| I-LoD | Amakhophi angu-1000/mL |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-TimeIzinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-TimeI-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
Ama-reagent okukhipha anconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ukukhipha kufanele kwenziwe ngokwe-IFU ngokuqinile. Umthamo wokususa onconyiwe ungu-80μL.
Inketho 2.
Ama-reagent okukhipha anconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3022) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ukukhipha kufanele kwenziwe ngokwe-IFU ngokuqinile. Umthamo wokususa onconyiwe ungu-100μL.
