Ikhithi isetshenziselwa ukutholwa kwe-PCR okusekelwe ku-fluorescence esezingeni eliphezulu yezingcezwana ze-nucleic acid eziqondene nezinhlobo eziyi-14 ze-human papillomavirus (HPV) (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68) kumaseli akhishwe ngomlomo wesibeletho kwabesifazane, kanye ne-HPV 16/18 genotyping ukusiza ukuxilonga nokwelapha ukutheleleka nge-HPV.