Ikhithi ye-RT-time ye-Fluorescent RT-PCR yokuthola i-SARS-CoV-2
Igama lomkhiqizo
I-HWTS-RT057A-real-time fluorescent rt-pcr kit ukuthola i-SARS-CoV-2
I-HWTS-RT057FF-IQHAWULEKI KOKUGCINA KWESIKHATHI SAMAHHALA SAMEMO SAMAHHALA WOKUTHOLA ISARS-CoV-2 -SUBPatage
Isitifiketi
CE
I-Epidemiology
I-coronavirus yenoveli (SARS-Cov-2) isabalale ngezinga elikhulu emhlabeni jikelele. Enqubweni yokusabalalisa, ukuguqulwa okusha kwenzeka njalo, okuholela ekuhlukaneni okusha. Lo mkhiqizo usetshenziselwa ukutholwa okusizayo kanye nokwehlukanisa amacala ahlobene nokutheleleka ngemuva kokusakazeka okukhulu kwe-alpha, beta, i-gamma, delta kanye nezinkinga ze-omicron mutant kusukela ngoDisemba 2020.
Insungubezi
| -Yenza | U-2019-NCOV Orf1ab gene |
| Cy5 | Ngo-2019-NCOV N Gene |
| I-VIC (Hex) | Uhlobo lwangaphakathi lwereferensi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | Uketshezi: ≤-18 ℃ ebumnyameni |
| I-Lyophile: ≤30 ℃ ebumnyameni | |
| Ishalofu-impilo | Uketshezi: izinyanga eziyi-9 |
| I-Lyophilised: Izinyanga eziyi-12 | |
| Uhlobo lwe-Specimen | I-Nasopharyngel Swabs, i-Oropharyngengeal Swabs |
| CV | ≤5.0% |
| Ct | ≤38 |
| Indlu edayisa insimbi | 300copies / ml |
| Ukucacisa | Akukho okuphambana nokusebenzisa imali ngama-coronaviveses womuntu sarS-CoV namanye amagciwane ajwayelekile. |
| Izinsimbi ezisebenzayo: | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR SLAN ®-96P real-time PCRS Systems I-QuantStudio ™ 5 Izinhlelo ze-PCR zangempela zesikhathi sangempela I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1.
Ukukhishwa kwe-nucleic acid noma ikhithi yokuhlanza (indlela yobuhlalu ye-magnetic) (HWTS-3001, i-HWTS-3004-32, i-HWS-3004-48) kusuka kuJiangsu Macro & Micro-Test Med-Tech Co, Ltd.
Inketho 2.
I-realent enconyiwe I-realent: I-QAAMP Viral RNA MINI KIT (52904), i-Viral RNA Extraction Kit (yppp35-R) ekhiqizwe yiTiangen Biotech (Beijing) Co, Ltd.
