Ikhithi ye-RT-PCR ye-fluorescent yesikhathi sangempela yokuthola i-SARS-CoV-2
Igama lomkhiqizo
Ikhithi ye-HWTS-RT057A-yesikhathi sangempela ye-RT-PCR yokuthola i-SARS-CoV-2
Ikhithi ye-RT-PCR ye-HWTS-RT057F-eyomisiwe nge-Real-time fluorescent RT-PCR yokuthola i-SARS-CoV-2 -Subpackage
Isitifiketi
CE
I-Epidemiology
I-coronavirus entsha (SARS-CoV-2) isakazeke ngezinga elikhulu emhlabeni jikelele. Enkambisweni yokusabalala, izinguquko ezintsha zihlala zenzeka, okuholela ezinhlotsheni ezintsha. Lo mkhiqizo usetshenziselwa kakhulu ukuthola nokuhlukanisa amacala ahlobene nokutheleleka ngemuva kokusabalala okukhulu kwezinhlobo ze-Alpha, Beta, Gamma, Delta kanye ne-Omicron mutant kusukela ngoDisemba 2020.
Isiteshi
| I-FAM | I-2019-nCoV ORF1ab gene |
| CY5 | I-2019-nCoV N gene |
| I-VIC(HEX) | i-gene yokubhekisela yangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ Ebumnyameni |
| I-Lyophilized: ≤30℃ Ebumnyameni | |
| Isikhathi sokuphelelwa yisikhathi | Uketshezi: izinyanga ezingu-9 |
| I-Lyophilized: izinyanga ezingu-12 | |
| Uhlobo Lwesifanekiso | ama-nasopharyngeal swabs, ama-oropharyngeal swabs |
| CV | ≤5.0% |
| Ct | ≤38 |
| I-LoD | Amakhophi angu-300/mL |
| Ukucaciswa | Akukho ukuhlangana kwezifo ezibangelwa yi-coronavirus yabantu i-SARS-CoV kanye nezinye izifo ezivamile. |
| Izinsimbi Ezisebenzayo: | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-SLAN ®-96P Zesikhathi Sangempela Izinhlelo ze-PCR ze-QuantStudio™ ezi-5 zesikhathi sangempela Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
Ikhithi Yokukhipha Noma Yokuhlanza I-Nucleic Acid (Indlela Yobuhlalu Be-Magnetic) (HWTS-3001, HWTS-3004-32, HWTS-3004-48) evela ku-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Inketho 2.
I-reagent yokukhipha enconywayo: I-QIAamp Viral RNA Mini Kit (52904), I-Viral RNA Extraction Kit (YDP315-R) eyenziwe yi-Tiangen Biotech (Beijing) Co., Ltd.
