Ikhithi ye-RT-PCR ye-fluorescent yesikhathi sangempela yokuthola i-SARS-CoV-2
Igama lomkhiqizo
Ikhithi ye-HWTS-RT057A-Real-time fluorescent RT-PCR yokuthola i-SARS-CoV-2
Ikhithi ye-HWTS-RT057F-Frize-eyomisiwe ye-Real-time fluorescent RT-PCR yokuthola i-SARS-CoV-2 -I-Subpackage
Isitifiketi
CE
I-Epidemiology
I-novel coronavirus (SARS-CoV-2) isabalale ngezinga elikhulu emhlabeni jikelele.Enqubweni yokusabalalisa, ukuguqulwa okusha kwenzeka njalo, okuholela ezinhlobonhlobo ezintsha.Lo mkhiqizo usetshenziselwa kakhulu ukutholwa nokuhlukaniswa kwamacala ahlobene nokutheleleka ngemva kokusabalala okukhulu kwezinhlobo ze-Alpha, Beta, Gamma, Delta ne-Omicron mutant kusukela ngoDisemba 2020.
Isiteshi
| FAM | 2019-nCoV ORF1ab gene |
| CY5 | 2019-nCoV N uhlobo |
| I-VIC(HEX) | isakhi sofuzo sereferensi yangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ ebumnyameni |
| I-Lyophilized: ≤30℃ ebumnyameni | |
| Impilo yeshelufu | Uketshezi: izinyanga ezingu-9 |
| I-Lyophilized: izinyanga eziyi-12 | |
| Uhlobo lwesifanekiso | i-nasopharyngeal swabs, i-oropharyngeal swabs |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 300Amakhophi/mL |
| Ukucaciswa | Akukho ukuhlangana kabusha nama-coronavirus abantu i-SARS-CoV namanye amagciwane ajwayelekile. |
| Izinsimbi Ezisebenzayo: | I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-SLAN ®-96P Real-Time PCR Systems I-QuantStudio™ 5 Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
I-Nucleic Acid Extraction noma Ikhithi Yokuhlanza (Indlela Yobuhlalu Bebuhlalu)(HWTS-3001, HWTS-3004-32, HWTS-3004-48) evela ku-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Inketho yesi-2.
Isisetshenziswa esisebenzayo sokumba esinconyiwe: I-QIAamp Viral RNA Mini Kit (52904), i-Viral RNA Extraction Kit (YDP315-R) ekhiqizwa i-Tiangen Biotech (Beijing) Co., Ltd.
