Amagciwane Okuphefumula Ahlanganisiwe

Incazelo emfushane:

Le kit isetshenziselwa ukutholwa kwe-in vitro qualitative yegciwane lomkhuhlane A, igciwane lomkhuhlane B, igciwane le-syncytial yokuphefumula, i-adenovirus, i-human rhinovirus kanye ne-mycoplasma pneumoniae nucleic acids kuma-swabs e-nasopharyngeal kanye namasampula e-oropharyngeal swab.Imiphumela yokuhlolwa ingasetshenziselwa ukusiza ekuxilongeni izifo ze-pathogen yokuphefumula, futhi inikeze isisekelo sokuxilonga samangqamuzana ukuze kuhlonzwe kanye nokwelashwa kwezifo ze-pathogen zokuphefumula.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-RT050-Izinhlobo Eziyisithupha Zekhithi Yokuthola I-Pathogen Nucleic Acid Yokuphefumula(Fluorescence PCR)

I-Epidemiology

Umkhuhlane, owaziwa ngokuthi 'umkhuhlane', isifo esithathelwanayo esinamandla sokuphefumula esibangelwa igciwane lomkhuhlane, elithathelanayo kakhulu futhi lisakazwa kakhulu ngokukhwehlela kanye nokuthimula.

I-Respiratory syncytial virus (RSV) igciwane le-RNA, eliyingxenye yomndeni we-paramyxoviridae.

I-Human adenovirus (HAdV) igciwane le-DNA elinemicu ekabili elingenayo imvilophu.Okungenani ama-genotypes angu-90 atholiwe, angahlukaniswa abe yi-7 subgenera AG.

I-Human rhinovirus (HRV) iyilungu lomndeni wakwa-Picornaviridae kanye nohlobo lwe-Enterovirus.

I-Mycoplasma pneumoniae (MP) iwuhlobo lwe-pathogenic microorganism ephakathi kwamagciwane namagciwane ngosayizi.

Isiteshi

Isiteshi	I-PCR-Mix A	I-PCR-Mix B
Isiteshi se-FAM	IFV A	I-HAdV
Isiteshi se-VIC/HEX	HRV	IFV B
Isiteshi se-CY5	I-RSV	MP
Isiteshi se-ROX	Ukulawula Kwangaphakathi	Ukulawula Kwangaphakathi

Imingcele Yezobuchwepheshe

Isitoreji	-18℃
Impilo yeshelufu	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	I-Oropharyngeal swab
Ct	≤35
LoD	500Amakhophi/mL
Ukucaciswa	1.Imiphumela yokuhlolwa kwe-cross-reactivity ibonise ukuthi akukho ukusabela okuphambene phakathi kwekhithi kanye ne-coronavirus yabantu i-SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, izinhlobo zegciwane leParainfluenza 1, 2, kanye no-3, i-Chlamydia pneumoniae, i-metapneumovirus yabantu, i-Enterovirus A, B, C, D, i-Epstein-Barr virus, igciwane lesimungumungwane, i-cytomegalovirus yabantu, i-Rotavirus, i-Norovirus, i-Mumps virus, i-Varicella-zoster virus, i-Legionella, i-Bordetella pertussis, i-Haemophilus influenzaecus, aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata, Pneumocystis jiroveci, Cryptococcus neoformans kanye ne-human genomic nucleic acids. 2.Amandla okunqanda ukuphazamiseka: i-Mucin (60mg/mL), i-10% (v/v) igazi lomuntu, i-phenylephrine (2mg/mL), i-oxymetazoline (2mg/mL), i-sodium chloride (enezivimbelakuvunda) (20mg/mL), i-beclomethasone ( 20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), Fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha-interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), cefprozil (40μg/mL), Meropenem (200mg/mL), levofloxacin (10μg/mL), kanye tobramycin (0.6mg/mL) zikhethelwe ukuhlolwa kokuphazamiseka, futhi imiphumela yabonisa ukuthi izinto eziphazamisayo ekugxilweni okungenhla azikho ukusabela kokuphazamiseka emiphumeleni yokuhlola yamagciwane.
Izinsimbi Ezisebenzayo	I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio^®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler^®480 uhlelo lwe-PCR yesikhathi sangempela LineGene 9600 Plus Real-Time PCR Detection Systems I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System