I-Nucleic Acid Yegciwane Lokuphefumula Elihambisana Nokuphefumula
Igama lomkhiqizo
Ikhithi Yokuthola I-Nucleic Acid Ye-HWTS-RT016-I-Respiratory Syncytial Virus (i-Fluorescence PCR)
I-Epidemiology
Igciwane le-Respiratory syncytial (RSV) liyigciwane le-RNA, elivela emndenini we-paramyxoviridae. Lidluliselwa ngamaconsi omoya kanye nokuxhumana okuseduze futhi liyimbangela eyinhloko yokutheleleka kwepheshana lokuphefumula eliphansi ezinganeni. Izinsana ezinegciwane le-RSV zingase zibe ne-bronchiolitis enzima kanye ne-pneumonia, okuhlobene ne-asthma ezinganeni. Izinsana zinezimpawu ezinzima, okuhlanganisa umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kanye ne-laryngitis, bese kuba yi-bronchiolitis kanye ne-pneumonia. Izingane ezimbalwa ezigulayo zingaba yinkimbinkimbi nge-otitis media, i-pleurisy kanye ne-myocarditis, njll. Ukutheleleka kwepheshana lokuphefumula eliphezulu kuyisibonakaliso esiyinhloko sokutheleleka kubantu abadala kanye nezingane ezindala.
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | i-nasopharyngeal swab, i-oropharyngeal swab |
| Ct | ≤38 |
| CV | <5.0% |
| I-LoD | 5Amakhophi angu-00/mL |
| Ukucaciswa | Akukho ukusabela okuhlangene lapho usebenzisa le khithi ukuthola ezinye izifo ezibangelwa ukuphefumula (i-coronavirus entsha i-SARS-CoV-2, i-human coronavirus SARSr-CoV, i-MERSr-CoV, i-HCoV-OC43, i-HCoV-229E, i-HCoV-HKU1, i-HCoV-NL63, izinhlobo zegciwane le-parainfluenza 1, 2, kanye ne-3, i-chlamydia pneumoniae, i-human metapneumovirus, i-enterovirus A, B, C, D, i-human metapneumovirus, igciwane le-Epstein-Barr, igciwane le-measles, i-human cytomegalovirus, i-rotavirus, i-norovirus, igciwane le-mumps, igciwane le-varicella-zoster, i-legionella, i-bordetella pertussis, i-haemophilus influenzae, i-staphylococcus aureus, i-streptococcus pneumoniae, i-streptococcus pyogenes, i-klebsiella pneumoniae, i-mycobacterium tuberculosis, i-aspergillus fumigatus, i-candida albicans, i-candida glabrata, i-pneumocystis jiroveci, i-cryptococcus neoformans) kanye ne-human i-DNA yezakhi zofuzo. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480, Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 Real-Time PCR. |
Ukugeleza Komsebenzi
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. iyanconywa ekukhishweni kwesampula futhi izinyathelo ezilandelayo kufanele zenziwe ngokuhambisana ne-IFU ye-Kit.
