I-SARS-CoV-2 influenza A influenza B Nucleic Acid Ihlanganisiwe
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-RT060A-SARS-CoV-2 Umkhuhlane A Umkhuhlane B I-Nucleic Acid Combined (i-Fluorescence PCR)
Isitifiketi
I-AKL/TGA/CE
I-Epidemiology
Isifo Segciwane Le-Corona sika-2019 (i-COVID-19) sibangelwa yi-SARS-CoV-2 engeyakwa-β Coronavirus yohlobo. I-COVID-19 iyisifo esithathelwanayo sokuphefumula esiyingozi, futhi isixuku ngokuvamile sisengozini. Njengamanje, iziguli ezine-SARS-CoV-2 zingumthombo oyinhloko wokutheleleka, kanti iziguli ezingenazo izimpawu zingase zibe umthombo wokutheleleka. Ngokusekelwe ocwaningweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku eziyi-1-14, ikakhulukazi izinsuku ezi-3-7. Izimpawu eziyinhloko kwakuwumkhuhlane, ukukhwehlela okomile kanye nokukhathala. Iziguli ezimbalwa zinezimpawu ezifana nokuminyana kwamakhala, ikhala eligobhozayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo.
I-Influenza iyisifo sokuphefumula esibuhlungu esibangelwa yigciwane le-influenza. Ithelelana kakhulu futhi isakazeka ikakhulukazi ngokukhwehlela nokuthimula. Ngokuvamile iqubuka entwasahlobo nasebusika. Kunezinhlobo ezintathu ze-Influenza, i-Influenza A (IFV A), i-Influenza B (IFV B) kanye ne-Influenza C (IFV C), zombili zingaphansi komndeni we-ortomyxovirus. I-Influenza A kanye ne-B, ezingama-RNA virus ahlukaniswe ngomugqa owodwa, ziyizimbangela eziyinhloko zezifo zabantu. I-Influenza A iyisifo esithathelwanayo sokuphefumula esibuhlungu, okuhlanganisa i-H1N1, i-H3N2 kanye nezinye izinhlobo, kulula ukushintsha. Ukuqubuka komhlaba wonke, "ukushintsha" kubhekisela ekuguqukeni komkhuhlane A, okuholela "kuhlobo oluncane" olusha lwegciwane. I-Influenza B ihlukaniswe ngezigaba ezimbili: i-Yamagata ne-Victoria. I-Influenza B ine-antigenic drift kuphela, futhi igwema ukubhekwa nokuqedwa yisistimu yomzimba yomuntu ngokuguqulwa. Kodwa amagciwane e-influenza B ashintsha kancane kakhulu kune-influenza A yabantu, ephinde ibangele izifo zokuphefumula kanye nobhubhane kubantu.
Isiteshi
| I-FAM | I-SARS-CoV-2 |
| I-ROX | I-IFV B |
| CY5 | I-IFV A |
| I-VIC(HEX) | Izakhi zofuzo zokulawula kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ Ebumnyameni |
| Ukwenziwa kwe-Lyophilization: ≤30℃ ebumnyameni | |
| Isikhathi sokuphelelwa yisikhathi | Uketshezi: izinyanga ezingu-9 |
| Ukuvuselelwa kwe-Lyophilization: izinyanga ezingu-12 | |
| Uhlobo Lwesifanekiso | Ama-swabs ase-Nasopharyngeal, ama-swabs ase-Oropharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-300/mL |
| Ukucaciswa | Imiphumela yokuhlolwa okuhlanganisiwe ikhombisile ukuthi ikhithi yayihambisana ne-coronavirus yomuntu i-SARSr-CoV, i-MERSr-CoV, i-HcoV-OC43, i-HcoV-229E, i-HcoV-HKU1, i-HCoV-NL63, igciwane le-syncytial lokuphefumula i-A ne-B, igciwane le-parainfluenza i-1, i-2 ne-3, i-rhinovirusA, i-B ne-C, i-adenovirus 1, i-2, i-3, i-4, i-5, i-7 ne-55, i-human metapneumovirus, i-enterovirus A, i-B, i-C ne-D, igciwane le-cytoplasmic pulmonary i-human, igciwane le-EB, igciwane le-measles i-Human cytomegalovirus, i-rotavirus, i-norovirus, igciwane le-mumps, igciwane le-varicella zoster, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniae, i-Legionella, i-pertussis, i-Haemophilus influenzae, i-Staphylococcus aureus, i-Streptococcus pneumoniae, i-Streptococcus pyogenes, i-Klebsiella pneumoniae, i-Mycobacterium tuberculosis, i-Aspergillus i-fumigatus, i-Candida albicans, i-Candida glabrata Akukho ukusabela okuhlangene phakathi kwe-Pneumocystis yersini kanye ne-Cryptococcus neoformans. |
| Izinsimbi Ezisebenzayo: | Ingafanelana namathuluzi e-PCR e-fluorescent ajwayelekile emakethe. Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
I-reagent yokukhipha enconywayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Inketho 2.
I-reagent yokukhipha enconyiwe: I-Nucleic Acid Extraction noma i-Purification Reagent (YDP302) yi-Tiangen Biotech (Beijing) Co.,Ltd.
