Igciwane Lomkhuhlane Wegazi laseXinjiang
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-FE007B/C I-Xinjiang Hemorrhagic Fever Virus Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Igciwane le-Xinjiang hemorrhagic fever laqala ukuhlukaniswa egazini leziguli ezine-hemorrhagic fever eTarim Basin, eXinjiang, eShayina kanye ne-hard ticks ezitholakale endaweni, futhi laqanjwa igama lalo. Izimpawu zemitholampilo zifaka phakathi umkhuhlane, ikhanda elibuhlungu, ukopha, ukushaqeka okucindezelayo, njll. Izinguquko eziyisisekelo zesifo salesi sifo ukwandiswa kwe-capillary systemic, ukuminyana, ukwanda kokungena kanye nobuthakathaka, okuholela emazingeni ahlukahlukene okuminyana kanye nokopha esikhumbeni kanye nasezithweni zangasese kanye nezicubu zezitho ezahlukahlukene emzimbeni wonke, kanye nokuwohloka kanye nokuqothuka kwezitho eziqinile njengesibindi, i-adrenal gland, i-pituitary gland, njll., kanye nokuvuvukala okufana ne-jelly ku-retroperitoneum.
Isiteshi
| I-FAM | Igciwane Lomkhuhlane Wegazi laseXinjiang |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | i-serum entsha |
| Tt | ≤38 |
| CV | ＜5.0% |
| I-LoD | Amakhophi angu-1000/mL |
| Ukucaciswa | Akukho ukuhlangana kwezifo nezinye izibonelo zokuphefumula ezifana ne-Influenza A, i-Influenza B, i-Legionella pneumophila, i-Rickettsia Q fever, i-Chlamydia pneumoniae, i-Adenovirus, i-Respiratory Syncytial Virus, i-Parainfluenza 1, 2, 3, i-Coxsackie virus, i-Echo virus, i-Metapneumovirus A1/A2/B1/B2, i-Respiratory syncytial virus A/B, i-Coronavirus 229E/NL63/HKU1/OC43, i-Rhinovirus A/B/C, i-Boca virus 1/2/3/4, i-Chlamydia trachomatis, i-adenovirus, njll. kanye ne-human genomic DNA. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhipha kufanele kwenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhipha. Ivolumu yesampula ekhishwe ingu-200µL, kanti ivolumu yokususa enconyiwe ingu-80µL.
I-reagent yokukhipha enconyiwe: I-QIAamp Viral RNA Mini Kit (52904) yi-QIAGEN kanye ne-Nucleic Acid Extraction noma i-Purification Reagent (YDP315-R). Ukukhipha kufanele kwenziwe ngokuhambisana nemiyalelo yokusebenzisa. Ivolumu yesampula ekhishwe ingu-140µL, kanti ivolumu yokususa enconyiwe ingu-60µL.
