I-Xinjiang Hemorrhagic Fever Virus
Igama lomkhiqizo
HWTS-FE007B/C Xinjiang Hemorrhagic Fever Virus Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Igciwane le-Xinjiang hemorrhagic fever laqala ukuhlukaniswa negazi leziguli ezinemfiva yokopha eTarim Basin, eXinjiang, eChina kanye nemikhaza eqinile ebanjwe endaweni, futhi yaqanjwa igama layo. Ukubonakaliswa kwemitholampilo kuhlanganisa imfiva, ikhanda elibuhlungu, ukopha, ukushaqeka kwe-hypotensive, njll. Izinguquko eziyisisekelo ze-pathological zalesi sifo yi-systemic capillary dilatation, ukuminyana, ukwanda kwe-permeability kanye nokuba ntekenteke, okuholela emazingeni ahlukene okuminyana kanye nokopha esikhumbeni kanye nolwelwesi lwamafinyila kanye nezicubu zezitho ezihlukahlukene emzimbeni wonke, ngokuwohloka kanye ne-necrosis enjalo, i-pillary gland, izitho eziqinile ze-adrenal. i-edema efana ne-jelly ku-retroperitoneum.
Isiteshi
| FAM | I-Xinjiang Hemorrhagic Fever Virus |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | i-serum entsha |
| Tt | ≤38 |
| CV | ＜5.0% |
| LoD | 1000Amakhophi/mL |
| Ukucaciswa | Akukho ukuphinda kusebenze namanye amasampula okuphefumula afana nomkhuhlane A, Umkhuhlane B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapnevirus A1/A1/A2u syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, njll. kanye ne-DNA yegenomic yomuntu. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela LineGene 9600 Plus Real-Time PCR Detection Systems I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ivolumu yesampula ekhishiwe ingu-200µL, futhi ivolumu ye-elution enconyiwe ngu-80µL.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-QIAamp Viral RNA Mini Kit (52904) nge-QIAGEN kanye ne-Nucleic Acid Extraction noma i-Purification Reagent (YDP315-R). Ukukhishwa kufanele kwenziwe ngokuhambisana ngokuqinile nemiyalelo yokusetshenziswa. Ivolumu yesampula ekhishiwe ingu-140µL, futhi ivolumu ye-elution enconyiwe ngu-60µL.
