Xinjiang hemorrhagic fever virus
Igama lomkhiqizo
I-HWTS-FE007B / C XINJIANG HEMORRHAGIC FUFTRAGH NUCLEIC Acid Detection Detection Kit (Fluorescence PCR)
I-Epidemiology
Igciwane le-Xinjiang hemorrhagic hemorrhagic fever i-virus lahlukaniswa okokuqala egazini leziguli ezinomkhuhlane we-hemorrhagic kuTarim Basin, XINJIANG, China kanye nemikhaza enzima ethwetshiwe endaweni yakini, futhi yathola igama. Ukubonakaliswa kwemitholampilo kufaka umkhuhlane, ikhanda, ukopha, ukushaqeka, njll. Izinguquko eziyisisekelo zalesi sifo, ukugcwala, ukukhuphuka kwamazinga ahlukahlukene nokugcwala kwezikhumba kanye Izicubu zezitho ezahlukahlukene emzimbeni wonke, ngokuwohloka kanye ne-necrosis yezitho eziqinile ezinjengesibindi, I-Adrenal Gland, i-Pituitary Gland, njll., kanye ne-edema enjengejelly ku-retropestitoneum.
Insungubezi
| -Yenza | Xinjiang hemorrhagic fever virus |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-9 |
| Uhlobo lwe-Specimen | i-serum entsha |
| Tt | ≤38 |
| CV | <I-5.0% |
| Indlu edayisa insimbi | 1000copies / ml |
| Ukucacisa | AKUKHO BANYE UKUXHUMANISA KWAMAHHALA namanye amasampula okuphefumula anjenge-A, Umkhuhlane B, Legisonalla q Fever, Adenovirus, 3, 3, i-Perapneumovirus A1 / A2 / B1 / B2, I-virus ebonakalayo yokuvumelanisa A / B, iCoronavirus 229E / NL63 / Hku1 / OC43, Rhinovirus A / B / C, i-Chlamydia Trachomatis, i-Adenovirus, njll. |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystem 7500 amasistimu we-PRCR wesikhathi sangempela, Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection Systems I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
I-realent enconyiwe I-realent: Macro & Micro-Vival DNA / RNA KIT (HWTS-3017) (angasetshenziswa nge-macro & micro-avin autory extractor (HWS-EQ011)) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd. Ukukhishwa kufanele kwenziwe ngokuya ngomyalo wokusebenzisa le mpendulo ye-extraction. Ivolumu yesampula ekhishwe i-200μL, futhi ivolumu yokuqedwa enconyiwe ingu-80μL.
I-realent enconyiwe I-realent: Qiaamp Viral Viral RNA KIT KIT (52904) nge-Qiagen Extraction ne-Nucleic Acid Extraction noma i-Reagent yokuhlanza (ypp15-R). Ukukhishwa kufanele kwenziwe ngokuqinile ngemiyalo yokusetshenziswa. IVolumu yesampula ekhishwe i-140μL, kanti ivolumu yokuqedwa enconyiwe ingu-60μL.
