Izinhlobo eziyi-14 ze-Human Papillomavirus enobungozi obukhulu (16/18/52 Typing)
Igama lomkhiqizo
HWTS-CC019A-Izinhlobo eziyi-14 ze-Human Papillomavirus (16/18/52 Typing) enobungozi obukhulu be-Nucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
Ucwaningo luye lwabonisa ukuthi izifo ezihlala njalo ze-HPV kanye nezifo eziningi zingenye yezimbangela eziyinhloko zomdlavuza womlomo wesibeletho.Njengamanje, izindlela zokwelapha ezisebenzayo ezibonwayo azikatholakali umdlavuza womlomo wesibeletho obangelwa i-HPV, ngakho ukutholakala kusenesikhathi kanye nokuvinjelwa kokutheleleka komlomo wesibeletho okubangelwa yi-HPV kuwukhiye wokuvimbela umdlavuza womlomo wesibeletho.Kubaluleke kakhulu ukusungula isivivinyo sokuxilonga i-etiology elula, eqondile futhi esheshayo ukuze kuhlonzwe umtholampilo kanye nokwelashwa komdlavuza womlomo wesibeletho.
Isiteshi
| Isiteshi | Uhlobo |
| FAM | I-HPV 18 |
| VIC/HEX | I-HPV 16 |
| I-ROX | I-HPV 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 |
| CY5 | I-HPV 52 |
| I-Quasar 705/CY5.5 | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Umchamo, I-Cervical Swab, I-Vaginal Swab |
| Ct | ≤28 |
| LoD | 300 Amakhophi/mL |
| Ukucaciswa | Akukho ukusabela okuphambene namanye amasampula okuphefumula afana nomkhuhlane A, Umkhuhlane B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneuvirus A1/A2 B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, njll. kanye ne-human genomic DNA. |
| Izinsimbi Ezisebenzayo | I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System kanye ne-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
1.Isampula yomchamo
A: Thatha1.4mL yesampula yomchamo okufanele ihlolwe kanye ne-centrifuge ku-12000rpm imizuzu emi-5;lahla i-supernatant (kunconywa ukuthi ugcine i-10-20μL supernatant kusukela phansi kweshubhu le-centrifuge), engeza u-200μL we-reagent yokukhulula isampula, futhi ukukhishwa okulandelayo kufanele kwenziwe ngokuvumelana nemiyalelo yokusetshenziswa kwe-Macro & Micro-Test Sample Release. I-Reagent (HWTS-3005-8).
B: Thatha1.4mL yesampula yomchamo okufanele ihlolwe kanye ne-centrifuge ku-12,000rpm imizuzu emi-5;lahla i-supernatant (kunconywa ukuthi ugcine u-10-20μL we-supernatant kusukela phansi kweshubhu le-centrifuge), bese wengeza u-200μL we-saline evamile ukuze umise futhi, njengesampula okufanele ihlolwe.Ukukhishwa okulandelayo kungenziwa nge-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & I-Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nge-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ngokuhambisana ngokuqinile nomyalelos ukusetshenziswa.Ivolumu ye-elution enconyiwe ngu-80μL.
C: Thatha1.4mL yesampula yomchamo okufanele ihlolwe kanye ne-centrifuge ku-12,000rpm imizuzu emi-5;lahla i-supernatant (kunconywa ukuthi ugcine u-10-20μL we-supernatant kusukela phansi kweshubhu le-centrifuge), futhi wengeze u-200μL wosawoti ovamile ukuze umise futhi, njengesampula okufanele ihlolwe.Ukukhishwa okulandelayo kungenziwa ngeI-QIAamp DNA Mini Kit(51304) nge-QIAGEN noma i-Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Ukukhishwa kufanele kucutshungulwe ngokulandela imiyalelo yokusetshenziswa.Ivolumu yesampula yokukhipha ingama-200μL, futhi ivolumu ye-elution enconyiwe ingu-80μL.
2. Isampula ye-swab yomlomo wesibeletho/i-vaginal swab
A: Thatha u-1mL wesampula ozohlolwa ku-1.5mLof i-centrifuge tube,futhiI-centrifuge ku-12000rpm imizuzu emi-5. Discard the supernatant (kunconywa ukuthi ugcine u-10-20μL we-supernatant kusukela phansi kweshubhu le-centrifuge), engeza u-100μL we-reagent yokukhulula isampula, bese ukhipha ngokulandela imiyalelo yokusebenzisa i-Macro & Micro-Test Sample Release Reagent ( HWTS-3005-8).
B: Ukukhishwa kungenziwa nge-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & I-Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nge-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ngokuhambisana ngokuqinile nemiyalelo yokusebenzisa.Ivolumu yesampula ekhishiwe ingu-200μL, futhi ivolumu ye-lution enconyiwe ingu-80μL.
C: Ukukhishwa kungenziwa nge-QIAamp DNA Mini Kit(51304) nge-QIAGEN noma i-Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Ukukhishwa kufanele kucutshungulwe ngokulandela imiyalelo yokusetshenziswa.Ivolumu yesampula yokukhipha ingu-200 μL, futhi ivolumu ye-lution enconyiwe ingu80 ml.
3, I-Cervical Swab/I-Vaginal Swab
Ngaphambi kokuthatha isampula, sebenzisa i-swab kakotini ukuze usule ngobumnene uketshezi okweqile emlonyeni wesibeletho, futhi usebenzise enye i-swab kakotini efakwe nesixazululo sokulondoloza amaseli noma ibhulashi lesampula lesampula leseli elikhishwe emlonyeni wesibeletho ukuze ubambelele ku-mucosa yomlomo wesibeletho bese ujika ngokulandela iwashi imijikelezo emi-3-5 ukuze uthole. amaseli e-exfoliated womlomo wesibeletho.Khipha kancane kancane i-swab kakotini noma ibhulashi,futhiyifake eshubhuni eliyisampula elino-1mL wosawoti ojwayelekile oyinyumba. Angemva kokuhlanza ngokugcwele, cindezela sula i-swab kakotini noma shayela odongeni lweshubhu bese ulahla, qinisa isivalo seshubhu, bese umaka igama lesampula (noma inombolo) bese uthayipha kushubhu yesampula.
