I-Mycobacterium Tuberculosis Rifampicin Resistance
Igama lomkhiqizo
HWTS-RT074A-Mycobacterium Tuberculosis Rifampicin Resistance Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Rifampicin ibisetshenziswa kabanzi ekwelapheni iziguli ezinesifo sofuba samaphaphu kusukela ngasekupheleni kwawo-1970, futhi inomphumela obalulekile.Kube yisinqumo sokuqala ukufushanisa ukwelashwa ngamakhemikhali ezigulini ze-pulmonary tuberculosis.Ukumelana ne-rifampicin kubangelwa ikakhulukazi ukuguqulwa kofuzo lwe-rpoB.Nakuba imishanguzo emisha yokulwa nesifo sofuba ihlale iphuma, kanye nokusebenza emtholampilo kweziguli ezinesifo sofuba samaphaphu nakho kuqhubekile nokuba ngcono, kusenokuntuleka kwezidakamizwa ezilwa nesifo sofuba, futhi ukwenzeka kokusetshenziswa kwezidakamizwa okungenangqondo emtholampilo kuphezulu uma kuqhathaniswa.Ngokusobala, isifo sofuba iMycobacterium ezigulini ezinesifo sofuba samaphaphu asikwazi ukubulawa ngokuphelele ngesikhathi esifanele, okuholela emazingeni ahlukene okumelana nemithi emzimbeni wesiguli, sandise isikhathi sokugula, futhi kwandisa ingozi yokufa kwesiguli.Le khithi ifanele ukuxilonga okuyisizayo kokutheleleka kwesifo sofuba i-Mycobacterium kanye nokutholwa kofuzo olumelana ne-rifampicin, olusiza ukuqonda ukumelana nemithi ye-mycobacterium tuberculosis etheleleke iziguli, kanye nokuhlinzeka ngezindlela ezisizayo zokuqondisa imithi yomtholampilo.
I-Epidemiology
| Igama Eliqondisiwe | Intatheli | Quencher | ||
| Isivimbeli sokusabelaA | Isivimbeli sokusabelaB | Isivimbeli sokusabelaC | ||
| I-rpoB 507-514 | I-rpoB 513-520 | IS6110 | FAM | Lutho |
| I-rpoB 520-527 | rpoB 527-533 | / | CY5 | Lutho |
| / | / | Ukulawula kwangaphakathi | I-HEX(VIC) | Lutho |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ ebumnyameni |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo lwesifanekiso | Isikhwehlela |
| CV | ＜5％ |
| LoD | uhlobo lwasendle olumelana ne-rifampicin: 2x103amagciwane/mL i-homozygous mutant: 2x103amagciwane/mL |
| Ukucaciswa | Ithola uhlobo lwasendle lwe-mycobacterium tuberculosis kanye nezindawo eziguqukayo zezinye izakhi zofuzo ezimelana nezidakamizwa ezifana ne-katG 315G>C\A, InhA-15C>T, imiphumela yokuhlola ikhombisa ukungamelani ne-rifampicin, okusho ukuthi akukho ukusabela okuphambene. |
| Izinsimbi Ezisebenzayo: | I-SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) |
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