Izinhlobo eziyi-15 ze-Human Papillomavirus E6/E7 Gene mRNA eyingozi kakhulu
Igama lomkhiqizo
Izinhlobo ze-HWTS-CC005A-15 ze-Human Papillomavirus E6/E7 Gene mRNA Detection Kit (i-Fluorescence PCR) ezisengozini enkulu
I-Epidemiology
Umdlavuza wesibeletho ungenye yezinhlobo ezivame kakhulu zomdlavuza wesifazane emhlabeni jikelele, futhi ukuvela kwawo kuhlobene kakhulu nama-human papillomavirus (HPV), kodwa ingxenye encane kuphela yokutheleleka kwe-HPV engakhula ibe umdlavuza. I-HPV eyingozi kakhulu ithelela amangqamuzana e-epithelial wesibeletho futhi ikhiqize ama-oncoprotein amabili, i-E6 ne-E7. Le phrotheni ingathinta izinhlobo ezahlukene zamaprotheni eseli (njengamaprotheni okucindezela isimila i-pRB kanye ne-p53), yandise umjikelezo weseli, ithinte ukwakheka kwe-DNA kanye nokuqina kwe-genome, futhi iphazamise izimpendulo zomzimba zokulwa namagciwane kanye nokulwa nesimila.
Isiteshi
| Isiteshi | Ingxenye | Kuhlolwe uhlobo lwe-genotype |
| I-FAM | I-HPV Reaction Buffer 1 | I-HPV16, 31, 33, 35, 51, 52, 58 |
| I-VIC/HEX | I-gene ye-β-actin yomuntu | |
| I-FAM | I-HPV Reaction Buffer 2 | I-HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| I-VIC/HEX | I-gene ye-INS yomuntu |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | I-swab yomlomo wesibeletho |
| Ct | ≤38 |
| CV | <5.0% |
| I-LoD | Amakhophi angu-500/mL |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-TimeIzinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhipha kufanele kwenziwe ngokuqinile ngokwemiyalelo yokusebenzisa. Umthamo wokususa onconyiwe ungu-50μL. Uma isampula ingagaywanga ngokuphelele, yibuyisele esinyathelweni sesi-4 ukuze iphinde igaywe. Bese uhlola ngokwemiyalelo yokusebenzisa.
I-reagent yokukhipha enconyiwe: I-RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431). Ukukhipha kufanele kwenziwe ngokwemiyalelo yokusebenzisa ngokuqinile (Esigabeni sesi-5, phinda kabili ukuhlushwa kwesisombululo esisebenzayo se-DNaseI, okungukuthi, thatha i-20μL yesisombululo sesitoko se-RNase-Free DNaseI (1500U) uyifake kushubhu entsha ye-RNase-Free centrifuge, engeza i-60μL ye-RDD buffer, bese uxuba kancane). Ivolumu yokususa enconyiwe ingu-60μL. Uma isampula ingagaywanga ngokuphelele, yibuyisele esinyathelweni sesi-5 ukuze iphinde igaywe. Bese uhlola ngokwemiyalelo yokusebenzisa.
