Izinhlobo eziyi-15 zengozi enkulu yabantu e-papillomavirus e6 / E7 gene Mrna

Incazelo emfushane:

Le kit ihlose ukutholwa kokufaneleka kobungozi obungu-15 obusengozini yobungozi obungu-15 be-e6 / e7 gene gene amazinga kumaseli we-exfoliated wesibeletho sowesifazane.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Izinhlobo ze-HWTS-CC005A-15 zengozi enkulu ye-papillomavirus E6 / E7 gene gene dction Kit (Fluorescence PCR)

I-Epidemiology

Umdlavuza wesibeletho ungenye yezinhlobo ezivame kakhulu zomdlavuza wesifazane emhlabeni wonke, futhi ukwenzeka kwalo kuhlobene kakhulu nama-papillomaviruses (HPV), kepha kuphela ingxenye encane yokutheleleka kwe-HPV engakhula ibe nomdlavuza. I-HPV ebeka engcupheni ephezulu ifaka amaseli wesibeletho somlomo wesibeletho futhi ikhiqize ama-oconicrote amabili, i-E6 ne-E7. Le protein ingathinta amaprotheni ahlukahlukene amaselula (anjenge-tumor supressor amaprotheni PRB ne-P53), anwebe umjikelezo weseli, athinte izimpendulo ze-genome ne-antitumoy.

Insungubezi

Insungubezi	Ingxenye	I-genotype ihlolwe
-Yenza	I-HPV RESERMENDE Buffer 1	HPV16,31,33,35,35,55,52,52,58
VIC / Hex	I-HPV RESERMENDE Buffer 1	Uhlobo lomuntu β-actin
-Yenza	I-HPV RESCOMER SACHERD 2	I-HPV 18,39,45,56,6,65,65.68
VIC / Hex	I-HPV RESCOMER SACHERD 2	UGene Womuntu Mshu

Amapharamitha Ezobuchwepheshe

Ukubeka	Uketshezi: ≤-18 ℃
Ishalofu-impilo	Izinyanga eziyi-9
Uhlobo lwe-Specimen	Isibeletho Swab
Ct	≤38
CV	<5.0%
Indlu edayisa insimbi	Amakhophi angama-500 / ml
Izinsimbi ezisebenzayo	Kusetshenziswe ama-biosystems 7500 uhlelo lwe-real-time PCRKusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®5 Systems real-time PCREMS SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR

Ukugeleza komsebenzi

I-reaglent enconyiwe ye-realent: I-Macro & Micro-Test Viral DNA / RNA KIT (HWTS-3020-50-HPV15) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd. Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngemiyalo yokusetshenziswa . IVolumu Ye-Expeated Enconyiwe ingu-50μL. Uma isampula lingagaywa ngokuphelele, libuyisele ku-Isinyathelo 4 ukuze silahlise. Bese uvivinya ngokuya ngemiyalo yokusebenzisa.

I-reaglent enconyelwe i-realent: I-RNApRep Pure Animal Tessue Tis all I-RNA Extraction Dealction Creduntaction Kit (DP431). Ukukhishwa kufanele kwenziwe ngokuya ngemiyalo yokusebenzisa ngokuqinile (esiteji 5, okuphindwe kabili kwe-DNASEI ukusebenza kwesixazululo se-DNASEI (1500U) isisombululo se-rnase-free tube, Faka i-60μL ye-RDD buffer, uhlanganise ngobumnene). IVolumu Ye-Expery Enconyiwe ingu-60μL. Uma isampula lingagaywa ngokuphelele, libuyisele ku-Isinyathelo 5 ngokunciphisa. Bese uvivinya ngokuya ngemiyalo yokusetshenziswa.

Okwedlule: I-PAPILLOMASIRUS YOMUNTU (28 izinhlobo) genityping

Olandelayo: I-HIV 1/2 Antibody

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