Izinhlobo eziyi-15 ze-Human Papillomavirus E6/E7 Gene mRNA
Igama lomkhiqizo
HWTS-CC005A-15 Izinhlobo ze-Human Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)
I-Epidemiology
Umdlavuza womlomo wesibeletho ungolunye lwezinhlobo ezivame kakhulu zomdlavuza wabesifazane emhlabeni wonke, futhi ukwenzeka kwawo kuhlobene eduze nama-human papillomaviruses (HPV), kodwa ingxenye encane kuphela yokutheleleka nge-HPV engakhula ibe umdlavuza.I-HPV enobungozi obukhulu ihlasela amaseli e-epithelial yomlomo wesibeletho futhi ikhiqize ama-oncoprotein amabili, i-E6 ne-E7.Le phrotheni ingathinta izinhlobonhlobo zamaphrotheni eselula (njengamaphrotheni e-tumor suppressor pRB kanye ne-p53), andise umjikelezo weseli, ithinte ukuhlanganiswa kwe-DNA nokuzinza kwe-genome, futhi iphazamise ukusabela kwe-antiviral kanye ne-antitumor immune.
Isiteshi
| Isiteshi | Isakhi | I-Genotype ihloliwe |
| FAM | I-HPV Reaction Buffer 1 | HPV16,31,33,35,51,52,58 |
| VIC/HEX | Isakhi sofuzo se-β-actin yomuntu | |
| FAM | I-HPV Reaction Buffer 2 | I-HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Isakhi sofuzo somuntu we-INS |
Imingcele Yezobuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo lwesifanekiso | I-swab yomlomo wesibeletho |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 Amakhophi/mL |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR SystemApplied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhishwa kufanele kwenziwe ngokulandela imiyalelo yokusetshenziswa. .Ivolumu ye-elution enconyiwe ngu-50μL.Uma isampula lingagayiwe ngokuphelele, libuyisele esinyathelweni sesi-4 ukuze ligaywe kabusha.Bese uvivinya ngokwemiyalelo yokusetshenziswa.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431).Ukukhishwa kufanele kwenziwe ngokulandela imiyalelo yokusetshenziswa ngokuqinile (Esinyathelweni sesi-5, phinda kabili ukugxilwa kwesisombululo sokusebenza se-DNaseI, okungukuthi, thatha u-20μL wesisombululo sesitoko se-RNase-Free DNaseI (1500U) uwafake kushubhu entsha ye-RNase-Free centrifuge, engeza u-60μL webhafa ye-RDD, bese uxuba ngobumnene).Ivolumu ye-elution enconyiwe ngu-60μL.Uma isampula lingagayiwe ngokuphelele, libuyisele esinyathelweni sesi-5 ukuze ligaywe kabusha.Bese uvivinya ngokwemiyalelo yokusetshenziswa.
