I-Chlamydia Trachomatis, Neisseria Gonorrhoeae kanye ne-Trichomonas vaginalis
Igama lomkhiqizo
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae and Trichomonas vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Chlamydia trachomatis (CT) iwuhlobo lwe-prokaryotic microorganism ene-parasitic eqinile kumaseli e-eukaryotic.I-Chlamydia trachomatis ihlukaniswe ngama-serotypes e-AK ngokuya ngendlela ye-serotype.Izifo ze-Urogenital tract ngokuvamile zibangelwa i-trachoma biological variant DK serotypes, futhi abesilisa babonakaliswa kakhulu njenge-urethritis, engakhululeka ngaphandle kokwelashwa, kodwa iningi labo liba elingapheli, ngezikhathi ezithile lithuthuke, futhi lingahlanganiswa ne-epididymitis, i-proctitis, njll.
Isiteshi
| FAM | I-Chlamydia trachomatis |
| I-ROX | I-Neisseria gonorrhoeae |
| CY5 | I-Trichomonal vaginitis |
| VIC/HEX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | -18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-swab yomlomo wesibeletho wesifazane,I-swab yesitho sangasese sowesifazane,I-swab ye-urethral yesilisa |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Amakhophi/mL |
| Ukucaciswa | Akukho ukuhlangana kabusha namanye amagciwane okutheleleka nge-STD ngaphandle kwebanga lokutholwa kwekhithi yokuhlola, njengeTreponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus type 1, Herpes simplex virus type 2, Candida albicans, njll. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Pipette 1mL yesampula okufanele ihlolwe ku-1.5mL ye-DNase/RNase-free centrifuge tube, i-centrifuge ku-12000rpm imizuzu emi-3, lahla amandla amakhulu futhi ugcine imvula.Engeza u-200µL we-saline evamile emvuleni ukuze umise futhi.I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhishwa kufanele kwenziwe ngokuvumelana nomyalelo wokusetshenziswa.Ivolumu yesampula ekhishiwe ingu-200µL, futhi ivolumu ye-elution enconyiwe ngu-80µL.
Inketho yesi-2.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Nucleic Acid Extraction noma Ikhithi Yokuhlanza (YDP302).Ukukhipha kufanele kwenziwe ngokuqinile ngokuvumelana nomyalelo wokusetshenziswa.Ivolumu ye-elution enconyiwe ngu-80µL.
