Izinhlobo ezingu-28 ze-HPV Nucleic Acid Detection Kit (i-Fluorescence PCR)
Igama lomkhiqizo
Izinhlobo ezingu-28 ze-HPV Nucleic Acid Detection Kit (i-Fluorescence PCR)
I-Epidemiology
I-Human Papillomavirus (HPV) ingeyomndeni we-Papillomaviridae wegciwane le-DNA eliyindilinga elinemigqa emibili, elingamboziwe, elinobude be-genome obungaba yizibili eziyisisekelo (bp) ezingu-8000. I-HPV ithelela abantu ngokuxhumana okuqondile noma okungaqondile nezinto ezingcolisiwe noma ukudluliselwa ngokocansi. Leli gciwane aligcini nje ngokuba ngelakhe, kodwa futhi liqondene nezicubu, futhi lingathelela kuphela isikhumba somuntu kanye namaseli e-epithelial e-mucosal, okubangela izinhlobo ezahlukene zama-papillomas noma ama-warts esikhumbeni somuntu kanye nomonakalo owandayo ku-epithelium yomgudu wokuzala.
Umdlavuza womlomo wesibeletho ungenye yezimila eziyingozi ezivame kakhulu ohlelweni lokuzala lwabesifazane. Izifundo zikhombisile ukuthi ukutheleleka okuqhubekayo kwe-HPV kanye nokutheleleka okuningi kungenye yezimbangela eziyinhloko zomdlavuza womlomo wesibeletho. Njengamanje, ukwelashwa okusebenzayo okuqashelwayo kusantuleka komdlavuza womlomo wesibeletho obangelwa yi-HPV, ngakho-ke ukutholakala kusenesikhathi nokuvimbela ukutheleleka komlomo wesibeletho okubangelwa yi-HPV kuyisihluthulelo sokuvimbela umdlavuza womlomo wesibeletho. Kubaluleke kakhulu ukusungula isivivinyo sokuxilonga esilula, esiqondile nesisheshayo se-etiology sokuxilongwa kanye nokwelashwa komdlavuza womlomo wesibeletho.
Isiteshi
| S/N | Isiteshi | Uhlobo |
| I-PCR-Mix1 | I-FAM | 16, 18, 31, 56 |
| I-VIC(HEX) | Ukulawula Kwangaphakathi | |
| CY5 | 45, 51, 52, 53 | |
| I-ROX | 33, 35, 58, 66 | |
| I-PCR-Mix2 | I-FAM | 6, 11, 54, 83 |
| I-VIC(HEX) | 26, 44, 61, 81 | |
| CY5 | 40, 42, 43, 82 | |
| I-ROX | 39, 59, 68, 73 |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤ -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isampula yomchamo, Isampula yeswabhu yomlomo wesibeletho yowesifazane, Isampula yeswabhu yowesifazane wesibeletho |
| CV | <5.0% |
| I-LoD | Amakhophi angu-300/mL |
| Ukucaciswa | Akukho ukusabela okuhlangene nezinye izifo ezibangelwa amagciwane ezihlobene nomgudu wokuzala kanye ne-DNA yezakhi zofuzo zomuntu. |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-Applied Biosystems 7500 Real-Time PCR, Izinhlelo ze-Applied Biosystems 7500 Fast Real-Time PCR, Izinhlelo ze-QuantStudio®5 Real-Time PCR, Izinhlelo ze-SLAN-96P Real-Time PCR (Hongshi Medical Technology Co., Ltd.), Izinhlelo ze-LightCycler®480 Real-Time PCR, Izinhlelo ze-LineGene 9600 Plus Real-Time PCR Detection (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 Real-Time PCR. |
Ukugeleza komsebenzi: Bheka i-Kit IFU ukuthola imininingwane
Isixazululo 1 (Ukukhishwa kwe-nucleic acid okuzenzakalelayo)
Isixazululo 2: (I-Macro & Micro-Test Sample Release Release Reagent)
Ama-reagent adinga kodwa awanikezwa
I-Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8), I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Ikholomu ye-Macro & Micro-Test Viral DNA/RNA (HWTS-3020-50).
Izinto ezisetshenziswayo ziyadingeka kodwa azinikezwa
Amashubhu angenawo i-DNase/RNase angu-1.5mL, amathiphu angenawo i-DNase/RNase, imichilo enamashubhu angu-8 ye-PCR, i-tabletop centrifuge, i-tabletop shaker mixer.
