I-ALDH Genetic Polymorphism
Igama lomkhiqizo
I-HWTS-GE015I-ALDH Genetic Polymorphism Detection Kit (ARMS -PCR)
I-Epidemiology
I-ALDH2 gene (i-acetaldehyde dehydrogenase 2), itholakala ku-chromosome yomuntu 12. I-ALDH2 ine-esterase, i-dehydrogenase nomsebenzi we-reductase ngesikhathi esifanayo. Ucwaningo luye lwabonisa ukuthi i-ALDH2 iyi-enzyme ye-nitroglycerin ye-metabolic, eguqula i-nitroglycerin ibe yi-nitric oxide, ngaleyo ndlela iphumuze imithambo yegazi futhi ithuthukise ukuphazamiseka kwegazi. Kodwa-ke, kukhona ama-polymorphisms kufuzo lwe-ALDH2, agxile kakhulu eMpumalanga Asia. I-ALDH2*1/*1 GG yohlobo lwasendle inamandla okugaywa kwe-metabolic, kuyilapho uhlobo lwe-heterozygous luno-6% kuphela womsebenzi we-enzyme yohlobo lwasendle, futhi uhlobo oluguquguqukayo lwe-homozygous lunomsebenzi we-enzyme ocishe ube ngu-zero, ne-metabolism ebuthakathaka kakhulu futhi ayikwazi ukufeza umphumela oyifunayo, ngaleyo ndlela ibangele ukulimala emzimbeni womuntu.
Isiteshi
| FAM | I-ALDH2 |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Igazi le-EDTA anticoagulated |
| CV | <5.0％ |
| LoD | 103Amakhophi/mL |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Ama-reagents okukhipha anconyiwe: sebenzisa i-Blood Genome DNA Extraction Kit (DP318) yi-Tiangen Biotech (Beijing) Co.,Ltd. noma i-Blood Genome Extraction Kit (A1120) nge-Promega ukuze kukhishwe i-EDTA i-anticoagulated blood Genomic DNA.
Ama-reagents okukhipha anconyiwe: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhishwa kufanele kwenziwe ngokuvumelana nemiyalelo eqinile. Ivolumu ye-elution enconyiwe ingu100μL.
