I-Aldh Genetic Polymorphism
Igama lomkhiqizo
I-HWTS-GE015I-ALDH Genetic Pelymorphism Detection Detection Kit (Arms -PCR)
I-Epidemiology
I-ALDH2 Gene (Acetaldehyde dehydrogenase 2), ezitholakala ku-chromosome yabantu 12. I-ALDH2 ine-EsdHogenase kanye nomsebenzi we-red restase ngasikhathi sinye. Ucwaningo luye lwabonisa ukuthi i-ALDH2 yi-enzyme ye-metabolic ye-nitroglycerin, eguqula i-nitroglycerin ibe yi-nitric oxide, ngaleyo ndlela ephumule imithambo yegazi nokuthuthukisa ukuphazamiseka kwegazi. Kodwa-ke, kukhona ama-polymorphisms kuhlobo lwe-ALDH2, olugxilwe kakhulu eMpumalanga Asia. Uhlobo lwe-Aldh2 * 1/1 GG lunekhono eliqinile le-metabolicolic, ngenkathi uhlobo lwe-heterozygous lunama-6% kuphela womsebenzi we-enzyme lwasendle, kanye nohlobo lwe-homozygous lusezingeni eliphakeme kakhulu le-zero enzyme, nge-metabolism ibuthakathaka kakhulu futhi ayikwazi ukufeza umphumela owufunayo, ngaleyo ndlela wabangela ukulimala komzimba womuntu.
Insungubezi
| -Yenza | I-ALDH2 |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | IGazi le-Edta anticoagulated |
| CV | <5.0% |
| Indlu edayisa insimbi | 103Amakhophi / ml |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 uhlelo lwe-real-time PCR Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
I-reagents yokukhishwa enconyiwe: Sebenzisa i-Blood Gene DNA Extraction Kit (D318) nguTiangen Biotech (Beijing) Co, Ltd. noma i-genome genome detection kit (A1120) nguPromega ukuze akhiphe i-Edta anticomic DNA yegazi.
I-reagents yokukhishwa enconyiwe: I-Macro & Micro-Test General DNA / RNA KIT (HWTS-3019) (engasetshenziswa nge-macro & micro-eq0 athomathikhi (HWS-EQ011)) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd. Ukukhishwa kufanele kwenziwe ngokuya ngemiyalo ngokuqinile. IVolumu Ye-Expeated Enconyiwe100μl.
