I-ALDH Genetic Polymorphism
Igama lomkhiqizo
I-HWTS-GE015Ikhithi Yokuthola I-ALDH Genetic Polymorphism (ARMS -PCR)
I-Epidemiology
I-ALDH2 gene (i-acetaldehyde dehydrogenase 2), itholakala ku-chromosome yomuntu 12. I-ALDH2 ine-esterase, i-dehydrogenase kanye nomsebenzi we-reductase ngesikhathi esifanayo. Izifundo zibonise ukuthi i-ALDH2 iyi-enzyme ye-metabolic ye-nitroglycerin, eguqula i-nitroglycerin ibe yi-nitric oxide, ngaleyo ndlela ikhulule imithambo yegazi futhi ithuthukise ukuphazamiseka kokugeleza kwegazi. Kodwa-ke, kunezinhlobo eziningi ze-enzyme ku-ALDH2 gene, ezigxile kakhulu eMpumalanga Asia. I-ALDH2*1/1 GG yohlobo lwasendle inekhono eliqinile le-metabolic, kuyilapho uhlobo lwe-heterozygous luno-6% kuphela womsebenzi we-enzyme yohlobo lwasendle, kanti uhlobo lwe-homozygous mutant alunawo umsebenzi we-enzyme cishe, kanti i-metabolism ibuthakathaka kakhulu futhi ayikwazi ukufeza umphumela oyifunayo, ngaleyo ndlela ibangele ukulimala emzimbeni womuntu.
Isiteshi
| I-FAM | I-ALDH2 |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Igazi elingasebenzi kahle le-EDTA |
| CV | <5.0％ |
| I-LoD | 103Amakhophi/mL |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Ama-reagent okukhipha anconyiwe: sebenzisa i-Blood Genome DNA Extraction Kit (DP318) yi-Tiangen Biotech (Beijing) Co., Ltd. noma i-Blood Genome Extraction Kit (A1120) yi-Promega ukuze ukhiphe i-EDTA yegazi elingasebenzi kahle i-Genomic DNA.
Ama-reagent okukhipha anconyiwe: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhipha kufanele kwenziwe ngokwemiyalelo ngokuqinile. Ivolumu yokususa enconyiwe ingu100μL.
