I-Mycoplasma Genitalium (Mg) Ikhithi Yokuthola I-Acid Yenucleic
Igama lomkhiqizo
I-Mycoplasma Genitalium (Mg) Ikhithi Yokuthola I-Acid Yenucleic
I-Epidemiology
Izifo ezithathelwana ngocansi (STD) zisalokhu zingenye yezinsongo ezinkulu ekuphepheni kwempilo yomphakathi emhlabeni wonke, okungaholela ekungazalini, ekuzalweni ngaphambi kwesikhathi, ekuveleni kwe-tumor, kanye nezinkinga eziningi ezinkulu [1-4]. Kunezinhlobo eziningi zezifo ezithathelwana ngocansi, okuhlanganisa amagciwane, amagciwane, i-chlamydia, i-mycoplasma, kanye nama-spirochetes. Izinhlobo ezivamile zifaka phakathi i-Neisseria gonorrhoeae, i-Chlamydia trachomatis, i-Ureaplasma urealyticum, i-Herpes simplex virus type 1, i-Herpes simplex virus type 2, i-Mycoplasma hominis, i-Mycoplasma genitalium kanye nokunye.
Isiteshi
| I-FAM | Mg |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | uukukhishwa kwe-rethral,ukukhiqizwa komlomo wesibeletho |
| Tt | ≤38 |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-500/μL |
| Ukucaciswa | Akukho ukuhlangana kwezifo ezithathelwana ngocansi nezinye izifo ezithathelwana ngocansi, njenge-Chlamydia trachomatis, i-Ureaplasma urealyticum, i-Neisseria gonorrhoeae, i-Mycoplasma hominis, i-Herpes simplex virus type 1, kanye ne-Herpes simplex virus type 2. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-TimeI-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukuhamba komsebenzi
Ama-reagent adinga kodwa awanikezwa
I-saline evamile, i-Macro kanye ne-Micro-Test Sample Release Release Reagent (HWTS-3005-8), i-Macro kanye ne-Micro-Test General DNA/RNA Kit (HWTS-3017) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B), i-Nucleic Acid Extraction noma i-Purification Reagent (YDP302) yi-Tiangen Biotech (Beijing) Co., Ltd.
Izinto ezisetshenziswayo ziyadingeka kodwa azinikezwa
Amashubhu e-centrifuge angenawo i-DNase/RNase angama-1.5 mL, amathiphu angenawo i-DNase/RNase, imichilo yamashubhu ayi-8 ye-PCR, i-pipette, i-desktop centrifuge, i-desktop oscillation mixer.
