Borrelia Burgdorferi nucleic acid
Igama lomkhiqizo
I-HWTS-OT076 Borrelia Burgdorferi nucleic acid ukutholwa (i-fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
Isifo seLyme sibangelwa ukutheleleka ngeBorrelia Burgdorferi futhi sidluliselwa ikakhulukazi phakathi kwabaphathi bezilwane, phakathi kwezilwane ezisingathwa kanye nabantu ngemikhaza kanzima. I-pathogen bolllelia burgdorferi ingadala ama-Ournerthemame Clorum Migran, kanye nezifo ezibandakanya izinhlelo eziningi ezifana nenhliziyo, izinzwa, kanye nokuhlangana, njll., Futhi ukubonakaliswa kwemitholampilo kuhlukahlukene. Ngokusho kokuthuthuka kwenkambo yezifo, kungahlukaniswa ekuthelelekeni kusenesikhathi kwasendaweni, ukutheleleka okusatshalaliswa okuphakathi nokutheleleka sekwedlule isikhathi, okulimaza kakhulu impilo yabantu. Ngakho-ke, ekuhlolweni komtholampilo kweBorrelia Burgdorferi, kubaluleke kakhulu ukusungula indlela elula, eqondile futhi esheshayo yokuxilongwa kwe-etiologia beBorrelia Burgdorferi.
Insungubezi
| -Yenza | I-DNA yeBorrelia Burgdorferi |
| VIC / Hex | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | Isampula yegazi lonke |
| Tt | ≤38 |
| CV | ≤5.0% |
| Indlu edayisa insimbi | Amakhophi angama-500 / ml |
| Izinsimbi ezisebenzayo | I-ABI 7500 real-time PCRS Systems I-ABI 7500 Fast Real-Time Pcr Systems I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1.
Qiaamp DNA Blood Midi Kit Kit (51185).It kufanele ikhishwengokuhambisana ngokuqinileemiyalweni, futhi ivolumu yokuqedwa enconyiwe100μl.
Inketho 2.
IgaziGI-Enomic DNAExtraction kit (dp38,Cha.: JingchangIrekhodi ledivayisi20210062) Kukhiqizwe yiTiangen Biochemical Technology (Beijing) Co, Ltd.. It kufanele ikhishwengokuhambisana ngokuqinileemiyalweni, futhi ivolumu yokuqedwa enconyiwe100μl.
Inketho 3.
I-Wizard® genomic DNA yokuhlanza ikhithi yokuhlanza (A1120) nguPromega.It kufanele ikhishwengokuhambisana ngokuqinileemiyalweni, futhi ivolumu yokuqedwa enconyiwe100μl.
