I-Borrelia Burgdorferi Nucleic Acid
Igama lomkhiqizo
I-HWTS-OT068 Borrelia Burgdorferi Nucleic Acid Detection Kit(Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
Isifo se-Lyme sibangelwa ukungenwa yi-Borrelia burgdorferi futhi sisakazwa kakhulu phakathi kwezilwane, phakathi kwezilwane ezihlala kuzo kanye nabantu ngemikhaza eqinile.I-pathogen i-Borrelia burgdorferi ingadala i-Erythema chronicum migrans yabantu, kanye nezifo ezibandakanya izinhlelo eziningi ezifana nenhliziyo, imizwa, kanye namalunga, njll., futhi ukubonakaliswa kwemitholampilo kuhlukahlukene.Ngokusho kokuthuthukiswa kwezifo, zingahlukaniswa zibe ukutheleleka kwasendaweni kwasekuqaleni, ukutheleleka okusakazwa okuphakathi kanye nokutheleleka okungapheli okungapheli, okulimaza kakhulu impilo yabantu.Ngakho-ke, ekuxilongweni komtholampilo kwe-Borrelia burgdorferi, kubaluleke kakhulu ukusungula indlela elula, eqondile futhi esheshayo yokuxilongwa kwe-etiological ye-Borrelia burgdorferi.
Isiteshi
| FAM | I-DNA ye-Borrelia burgdorferi |
| VIC/HEX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo lwesifanekiso | Isampula yegazi lonke |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Amakhophi/mL |
| Izinsimbi Ezisebenzayo | I-ABI 7500 Real-Time PCR Systems ABI 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
I-QIAamp DNA Blood Midi Kit ka-Qiagen (51185).It kufanele ikhishwengokuvumelana ngokuqinileemfundweni, kanye nevolumu yokwelulekwa enconyiwe100μL.
Inketho yesi-2.
IgaziGiDNA enomicEikhithi yokukhipha (DP318,Cha.: JingchangIrekhodi ledivayisi20210062) ekhiqizwe i-Tiangen Biochemical Technology (Beijing) Co., Ltd.. It kufanele ikhishwengokuvumelana ngokuqinileemfundweni, kanye nevolumu yokwelulekwa enconyiwe100μL.
Inketho yesi-3.
I-Wizard® Genomic DNA Purification Kit (A1120) yi-Promega.It kufanele ikhishwengokuvumelana ngokuqinileemfundweni, kanye nevolumu yokwelulekwa enconyiwe100μL.
