I-Borrelia Burgdorferi Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-OT076 Borrelia Burgdorferi Nucleic Acid (i-Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
Isifo seLyme sibangelwa ukutheleleka ngeBorrelia burgdorferi futhi sidluliselwa kakhulu phakathi kwezilwane ezihlala kuzo, phakathi kwezilwane ezihlala kuzo kanye nabantu ngama-hard tick. I-pathogen iBorrelia burgdorferi ingabangela i-Erythema chronicum migrans yabantu, kanye nezifo ezihilela izinhlelo eziningi ezifana nenhliziyo, imizwa, kanye namalunga, njll., futhi izimpawu zemitholampilo ziyahlukahluka. Ngokusho kokukhula kwezifo, ingahlukaniswa ngokutheleleka kwasendaweni kwasekuqaleni, ukutheleleka okusabalele phakathi kanye nokutheleleka okuqhubekayo okungapheli, okulimaza kakhulu impilo yabantu. Ngakho-ke, ekuxilongweni kweBorrelia burgdorferi emtholampilo, kubaluleke kakhulu ukusungula indlela elula, eqondile futhi esheshayo yokuxilonga iBorrelia burgdorferi.
Isiteshi
| I-FAM | I-DNA yeBorrelia burgdorferi |
| I-VIC/HEX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isampula yegazi eliphelele |
| Tt | ≤38 |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-500/mL |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-ABI 7500 Zesikhathi Sangempela Izinhlelo ze-PCR ze-ABI 7500 ze-Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
I-QIAamp DNA Blood Midi Kit kaQiagen (51185).It kufanele kukhishwengokuvumelana ngokuqinileemyalezweni, futhi ivolumu yokuxazulula enconywayo ingu100μL.
Inketho 2.
IgaziGi-DNA ye-enomicEikhithi yokukhipha (DP318,Cha.: JingchangIrekhodi Ledivayisi20210062) ekhiqizwe yiTiangen Biochemical Technology (Beijing) Co., Ltd.. It kufanele kukhishwengokuvumelana ngokuqinileemyalezweni, futhi ivolumu yokuxazulula enconywayo ingu100μL.
Inketho 3.
Ikhithi Yokuhlanza I-DNA Ye-Wizard® Genomic (A1120) yi-Promega.It kufanele kukhishwengokuvumelana ngokuqinileemyalezweni, futhi ivolumu yokuxazulula enconywayo ingu100μL.
