Izinhlobo eziyi-17 ze-Human Papillomavirus (16/18/6/11/44 Ukuthayipha) Ikhithi Yokuthola I-Nucleic Acid (i-Fluorescence PCR)
Igama lomkhiqizo
Izinhlobo eziyi-17 ze-Human Papillomavirus (16/18/6/11/44 Ukuthayipha) Ikhithi Yokuthola I-Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
I-Human Papillomavirus (HPV) ingeyomndeni we-Papillomaviridae wegciwane le-DNA eliyindilinga elinemigqa emibili, elingamboziwe, elinobude be-genome obungaba yizibili eziyisisekelo (bp) ezingu-8000. I-HPV ithelela abantu ngokuxhumana okuqondile noma okungaqondile nezinto ezingcolisiwe noma ukudluliselwa ngokocansi. Leli gciwane aligcini nje ngokuba ngelakhe, kodwa futhi liqondene nezicubu, futhi lingathelela kuphela isikhumba somuntu kanye namaseli e-epithelial e-mucosal, okubangela izinhlobo ezahlukene zama-papillomas noma ama-warts esikhumbeni somuntu kanye nomonakalo owandayo ku-epithelium yomgudu wokuzala.
Umdlavuza womlomo wesibeletho ungenye yezimila eziyingozi ezivame kakhulu endleleni yokuzala yabesifazane. Kuye kwabonakala ukuthi ukutheleleka kwe-HPV okuqhubekayo kanye nokutheleleka okuningi kungenye yezimbangela ezinkulu zomdlavuza womlomo wesibeletho. Okwamanje kusenokuntuleka kwezindlela zokwelapha ezisebenzayo ezamukelwa kabanzi zomdlavuza womlomo wesibeletho obangelwa yi-HPV. Ngakho-ke, ukutholakala kusenesikhathi nokuvimbela ukutheleleka komlomo wesibeletho okubangelwa yi-HPV kuyizihluthulelo zokuvimbela umdlavuza womlomo wesibeletho. Ukusungulwa kwendlela yokuxilonga elula, eqondile futhi esheshayo kubaluleke kakhulu ekuxilongweni komdlavuza womlomo wesibeletho emtholampilo.
Amapharamitha Obuchwepheshe
| Isitoreji | ≤ -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isampula yomchamo, Isampula yeswabhu yomlomo wesibeletho yowesifazane, Isampula yeswabhu yowesifazane wesibeletho |
| CV | <5.0% |
| I-LoD | Amakhophi angu-300/mL |
| Ukucaciswa | Akukho ukusabela okuhlangene nezinye izifo ezibangelwa amagciwane ezihlobene nomgudu wokuzala kanye ne-DNA yezakhi zofuzo zomuntu. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-Applied Biosystems 7500 Real-Time PCR, Izinhlelo ze-Applied Biosystems 7500 Fast Real-Time PCR, Izinhlelo ze-QuantStudio®5 Real-Time PCR, Izinhlelo ze-SLAN-96P Real-Time PCR (Hongshi Medical Technology Co., Ltd.), Izinhlelo ze-LightCycler®480 Real-Time PCR, Izinhlelo ze-LineGene 9600 Plus Real-Time PCR Detection (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), i-BioRad CFX96 Real-Time PCR System kanye ne-BioRad CFX Opus 96 Real-Time PCR System. |
Ukugeleza komsebenzi: Bheka i-Kit IFU ukuthola imininingwane
Isixazululo 1 (Ukukhishwa kwe-nucleic acid okuzenzakalelayo)
Isixazululo 2: (I-Macro & Micro-Test Sample Release Release Reagent)
Ama-reagent adinga kodwa awanikezwa
I-Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8), I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Ikholomu ye-Macro & Micro-Test Viral DNA/RNA (HWTS-3020-50).
Izinto ezisetshenziswayo ziyadingeka kodwa azinikezwa
Amashubhu e-centrifuge angenayo i-DNase/RNase angu-1.5mL, Amathiphu angenayo i-DNase/RNase, imichilo enamashubhu angu-8 e-PCR, i-desktop centrifuge, i-desktop vortex mixer.
