I-Coxsackie Virus Uhlobo lwe-A16 Nucleic Acid

Le kit yamukela i-Enzymatic Probe Isothermal Amplification (EPIA), futhi yakha ama-primers athile kanye ne-RNA base-containing probes (rProbe) yesifunda esilondolozwe kakhulu se-Cox A16, futhi yengeza i-Bst enzyme kanye ne-enzyme ye-RNaseH ngesikhathi esifanayo, lapho kwesokunxele kanye iziphetho ezingakwesokudla zesisekelo se-RNA se-rProbe zilebulwe ngamaqembu e-fluorescent nesicimi, ngokulandelanayo.Sebenzisa umsebenzi we-DNA polymerase kanye nomsebenzi wokususwa kwe-strand we-enzyme ye-Bst ukuze ukhulise ithagethi ukuze ihlolwe ezingeni lokushisa elingaguquki, i-enzyme ye-RNaseH ingakwazi ukuhlukanisa izisekelo ze-RNA kuchungechunge lwe-hybrid-probe hybrid, ukuze iqembu le-fluorescent kanye nesicisha se-rProbe kube. ihlukaniswe ngaleyo ndlela i-fluorescing.Ngaphezu kwalokho, ucezu lwesokunxele lwesizinda se-rProbe RNA esisele singasetshenziswa njengesiqalo ukuze sinwetshwe ukuze sakhe umkhiqizo, ophinde uqongelele umkhiqizo.Isignali ye-fluorescent iqoqwa ngokuqhubekayo ngokwakhiwa komkhiqizo, ngaleyo ndlela ibona ukutholwa kwe-nucleic acid ehlosiwe.