I-MTHFR Gene Polymorphic Nucleic Acid

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola izindawo ezi-2 zokuguqulwa kofuzo lwe-MTHFR.Ikhithi isebenzisa igazi eliphelele lomuntu njengesampula lokuhlola ukuze linikeze ukuhlolwa kwekhwalithi kwesimo soguquko.Ingasiza odokotela ukuthi baklame izinhlelo zokwelashwa ezifanele izici zomuntu ngamunye kusukela ezingeni lamangqamuzana, ukuze kuqinisekiswe impilo yeziguli ngezinga elikhulu kakhulu.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-GE004-MTHFR Gene Polymorphic Nucleic Acid Detection Kit (ARMS-PCR)

I-Epidemiology

I-Folic acid iyivithamini encibilika emanzini eyi-cofactor ebalulekile emigudwini ye-metabolic yomzimba.Eminyakeni yamuva nje, inani elikhulu locwaningo liqinisekisile ukuthi, ukuguqulwa kwe-folate metabolizing enzyme gene MTHFR kuzoholela ekuntulekeni kwe-folic acid emzimbeni, futhi umonakalo ovamile wokuntuleka kwe-folic acid kubantu abadala kungase kubangele i-megaloblastic anemia, i-vascular. ukulimala kwe-endothelial, njll. Ukuntuleka kwe-folic acid kwabesifazane abakhulelwe abakwazi ukuhlangabezana nezidingo zabo kanye ne-fetus, okungabangela ukukhubazeka kwe-neural tube, i-anencephaly, ukuzala, nokuphuphuma kwesisu.Amazinga we-serum folate athintwa yi-5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms.Ukuguqulwa kwe-677C>T kanye ne-1298A>C kufuzo lwe-MTHFR kudala ukuguqulwa kwe-alanine ku-valine ne-glutamic acid, ngokulandelana, okuholela ekunciphiseni komsebenzi we-MTHFR futhi ngenxa yalokho kunciphisa ukusetshenziswa kwe-folic acid.

Isiteshi

FAM I-MTHFR C677T
I-ROX I-MTHFR A1298C
I-VIC(HEX) Ukulawula kwangaphakathi

Imingcele Yezobuchwepheshe

Isitoreji

≤-18℃

Impilo yeshelufu

Izinyanga ezingu-12

Uhlobo lwesifanekiso

Igazi elisanda kuqoqwa i-EDTA anticoagulated

CV

≤5.0%

Ct

≤38

LoD

1.0ng/μL

Izinsimbi Ezisebenzayo:

I-Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

I-SLAN ®-96P Real-Time PCR Systems

I-QuantStudio™ 5 Real-Time PCR Systems

Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

I-LineGene 9600 Plus Real-Time PCR Detection System

I-MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Real-Time PCR System

I-BioRad CFX Opus 96 Real-Time PCR System

Ukugeleza komsebenzi

Inketho 1

Ama-reagents okukhipha anconyiwe: I-Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006C, HWTS-300) .

Inketho yesi-2

Ama-reagents okukhipha anconyiwe: Ikhithi yokukhipha i-Blood Genomic DNA(YDP348, JCXB20210062) yi-Tiangen Biotech(Beijing) Co.,Ltd.Ikhithi yokukhipha iGenome yegazi(A1120) nge-Promega.


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