Igciwane le-Dengue, i-virus virus ne-chikungunya virus Multiplex
Igama lomkhiqizo
Igciwane le-HWTS-FE040 dengue, virus virus virus kanye ne-chikungunya virus Multiplex nucleic acid detection detection (Fluorescence PCR)
I-Epidemiology
I-Dengue Fever (DF), edonswa ukutheleleka nge-dengue virus (Denv), ingenye yezifo eziwubhaliwe kakhulu i-Arbovirus izifo ezithathelwanayo. Okuphakathi kwalo okuphakathi kufaka phakathi i-AEDES AEGYPTI ne-AEDES allopictus. I-DF ivame kakhulu ezindaweni ezishisayo nezingaphansi komhlaba. I-Denv ingeye-flavivirirus ngaphansi kwe-flaviviridae, futhi ingahlukaniswa ibe ama-serotypes ama-4 ngokuya nge-antigen engaphezulu. Ukubonakaliswa kwemitholampilo kokutheleleka kweDenV kufaka phakathi ikhanda, umkhuhlane, ubuthakathaka, ukukhulisa kwe-lymph node, i-leukopenia kanye nokunye ukulimala noma ukufa kwe-hepatic noma ukufa ezimweni ezinzima. Eminyakeni yamuva nje, ukuguquguquka kwesimo sezulu, ukuthuthukiswa kwamadolobha, ukuthuthukiswa kwezokuvakasha kanye nezinye izinto kuhlinzeke ngezimo ezisheshayo nezilula zokudlulisela nokusakazeka kwe-DF, okuholela ekunwetshisweni njalo kwendawo ye-epidemic ye-DF.
Insungubezi
| -Yenza | I-Denv nucleic acid |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | -18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-9 |
| Uhlobo lwe-Specimen | I-serum entsha |
| Ct | ≤38 |
| CV | <5% |
| Indlu edayisa insimbi | Amakhophi angama-500 / ml |
| Ukucacisa | Imiphumela yokuhlola ukuphazamiseka ikhombisa ukuthi lapho ukugcwala kwe-bilirubin ku-serum kungabi ngaphezu kwe-168.2μmol / ml, i-hemoglobin okuhlushwa okukhiqizwa yi-hemolysis akuphezulu kwe-130mmol Ukuhlushwa ku-serum akukubi ngaphezu kwe-5mg / ml, akukho mthelela egciwane le-dengue, igciwane le-zika noma Ukutholwa kwegciwane le-chikungunya. I-Hepatitis I-Virus, i-Hepatitis B Virus, i-Hepatitis C Virus, i-herpes virus, i-Eastern, i-Bunya virus, amasampula e-West Nile kanye ne-Suman genomic serum akhethiwe ukuhlolwa kwe-cross-genomic serum, futhi imiphumela ikhombisa ukuthi akukho ukusabela kwe-cross phakathi kwale kit kanye namagciwane ashiwo ngenhla. |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 uhlelo lwe-real-time PCR Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1.
I-Tinamp Virus DNA / RNA KIT (yppp15-R), futhi ukukhishwa kufanele kwenziwe ngokuqinile ngomyalo wokusetshenziswa. IVolumu yesampula ekhishwe i-140μL, kanti ivolumu yokuqedwa enconyiwe ingu-60μL.
Inketho 2.
I-Macro & Micro-Test General DNA / RNA KIT (HWTS-3017-50, HWS-3017-50, HWTS-3017-48, HWS-3017-48, HWS-3017-46, HWS-3017-96, I-NUCLEIC AUCLEIC autholt extractor (HWTS-3006C, HWTS-3006B)) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd., kanye nokukhishwa kufanele kwenziwe ngokuya ngomyalo wokusebenzisa. Ivolumu yesampula ekhishwe i-200μL, futhi ivolumu yokuqedwa enconyiwe ingu-80μL.
