Igciwane le-Dengue, igciwane le-Zika kanye ne-Chikungunya Virus Multiplex
Igama lomkhiqizo
I-HWTS-FE040 Dengue Virus, Zika Virus kanye neChikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Umkhuhlane we-dengue (DF), obangelwa ukutheleleka yi-dengue virus (DENV), ungenye yezifo ezithelelanayo ze-arbovirus eziwubhubhane kakhulu. Indlela yawo yokudlulisela ihlanganisa i-Aedes aegypti kanye ne-Aedes albopictus. I-DF ivame kakhulu ezindaweni ezishisayo nezishisayo. I-DENV ingeyama-flavivirus ngaphansi kwe-flaviviridae, futhi ingahlukaniswa ngama-serotypes amane ngokuya nge-antigen engaphezulu. Izimpawu zemitholampilo zokutheleleka kwe-DENV zifaka phakathi ikhanda elibuhlungu, umkhuhlane, ubuthakathaka, ukwanda kwe-lymph node, i-leukopenia njll., kanye nokopha, ukushaqeka, ukulimala kwesibindi noma ngisho nokufa ezimweni ezimbi kakhulu. Eminyakeni yamuva nje, ushintsho lwesimo sezulu, ukusabalala kwamadolobha, intuthuko esheshayo yezokuvakasha kanye nezinye izici kuhlinzeke ngezimo ezisheshayo nezilula zokudlulisa nokusabalala kwe-DF, okuholela ekwandeni okuqhubekayo kwendawo yobhubhane ye-DF.
Isiteshi
| I-FAM | I-DENV nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | I-serum entsha |
| Ct | ≤38 |
| CV | ＜5% |
| I-LoD | Amakhophi angu-500/mL |
| Ukucaciswa | Imiphumela yokuhlolwa kokuphazamiseka ikhombisa ukuthi uma ukugcwala kwe-bilirubin ku-serum kungaphezu kuka-168.2μmol/ml, ukugcwala kwe-hemoglobin okukhiqizwa yi-hemolysis kungaphezu kuka-130g/L, ukugcwala kwamafutha egazini kungaphezu kuka-65mmol/ml, ukugcwala okuphelele kwe-IgG ku-serum kungaphezu kuka-5mg/mL, akukho mphumela egciwaneni le-dengue, ekutholakaleni kwegciwane le-Zika noma igciwane le-chikungunya. Igciwane le-Hepatitis A, igciwane le-Hepatitis B, igciwane le-Hepatitis C, igciwane le-Herpes, igciwane le-Eastern equine encephalitis, igciwane le-Hantavirus, igciwane le-Bunya, igciwane le-West Nile kanye namasampula e-serum ye-genomic yabantu akhethiwe ukuze kuhlolwe i-cross-reactivity, futhi imiphumela ikhombisa ukuthi akukho ukusabela okuhlangene phakathi kwale kit kanye namagciwane okukhulunywe ngawo ngenhla. |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
I-TIANamp Virus DNA/RNA Kit (YDP315-R), futhi ukukhishwa kufanele kwenziwe ngokuhambisana ngqo nemiyalelo yokusebenzisa. Umthamo wesampula okhishwe ngu-140μL, kanti umthamo wokuhlanza onconywayo ngu-60μL.
Inketho 2.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., futhi ukukhishwa kufanele kwenziwe ngokwemiyalelo yokusetshenziswa. Umthamo wesampula okhishwe ungama-200μL, kanti umthamo wokulungisa onconywayo ungama-80μL.
