I-Dengue Virus, Zika Virus kanye neChikungunya Virus Multiplex
Igama lomkhiqizo
I-HWTS-FE040 Dengue Virus, Zika Virus kanye neChikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Dengue fever (DF), ebangelwa ukutheleleka kwe-dengue virus (DENV), ingesinye sezifo ezithathelwanayo ze-arbovirus eziwubhubhane.I-transmission medium ihlanganisa i-Aedes aegypti ne-Aedes albopictus.I-DF ivame kakhulu ezindaweni ezishisayo nezishisayo.I-DENV ingeye-flavivirus engaphansi kwe-flaviviridae, futhi ingahlukaniswa ngama-serotypes angu-4 ngokuya nge-surface antigen.Ukubonakaliswa komtholampilo kokutheleleka kwe-DENV ikakhulukazi kuhlanganisa ikhanda, imfiva, ubuthakathaka, ukwanda kwe-lymph node, i-leukopenia nokunye, nokopha, ukushaqeka, ukulimala kwesibindi noma ngisho nokufa ezimweni ezimbi kakhulu.Eminyakeni yamuva nje, ukuguquguquka kwesimo sezulu, ukufudukela kwabantu emadolobheni, ukuthuthuka okusheshayo kwezokuvakasha kanye nezinye izici zinikeze izimo ezisheshayo nezilula zokudlulisa nokusabalalisa i-DF, okuholela ekwandeni okuqhubekayo kwendawo ewubhubhane ye-DF.
Isiteshi
| FAM | MP nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | -18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | I-serum entsha |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Amakhophi/mL |
| Ukucaciswa | Imiphumela yokuhlolwa kokugxambukela ikhombisa ukuthi lapho ukugcwala kwe-bilirubin ku-serum ingekho ngaphezu kwe-168.2μmol/ml, ukugcwala kwe-hemoglobin ekhiqizwa yi-hemolysis akukho ngaphezu kwe-130g/L, ukugcwala kwe-lipid yegazi akukho ngaphezu kwama-65mmol/ml, ingqikithi ye-IgG. ukugxila ku-serum akudluli ku-5mg/mL, awukho umthelela kugciwane lodenga, igciwane leZika noma ukutholwa kwegciwane le-chikungunya.Igciwane le-Hepatitis A, igciwane le-Hepatitis B, igciwane le-Hepatitis C, i-Herpes virus, i-Eastern equine encephalitis virus, i-Hantavirus, igciwane le-Bunya, igciwane le-West Nile kanye namasampula e-genomic serum yabantu akhethiwe ukuze kuhlolwe i-cross-reactivity, futhi imiphumela ibonisa ukuthi akukho ukungezwani phakathi kwaleli kit kanye namagciwane ashiwo ngenhla. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
I-TIANamp Virus DNA/RNA Kit (YDP315-R), futhi ukukhipha kufanele kwenziwe ngokuhambisana ngokuqinile nomyalelo wokusetshenziswa.Ivolumu yesampula ekhishiwe ingu-140μL, futhi ivolumu ye-lution enconyiwe ingu-60μL.
Inketho yesi-2.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., futhi ukukhishwa kufanele kwenziwe ngokuvumelana nomyalelo wokusetshenziswa.Ivolumu yesampula ekhishiwe ingu-200μL, futhi ivolumu ye-lution enconyiwe ingu-80μL.
