I-Dengue Virus, Zika Virus kanye neChikungunya Virus Multiplex
Igama lomkhiqizo
I-HWTS-FE040 Dengue Virus, Zika Virus kanye neChikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Dengue fever (DF), ebangelwa ukutheleleka kwe-dengue virus (DENV), ingesinye sezifo ezithathelwanayo ze-arbovirus eziwubhubhane. I-transmission medium ihlanganisa i-Aedes aegypti ne-Aedes albopictus. I-DF ivame kakhulu ezindaweni ezishisayo nezishisayo. I-DENV ingeye-flavivirus engaphansi kwe-flaviviridae, futhi ingahlukaniswa ngama-serotypes angu-4 ngokuya nge-surface antigen. Ukubonakaliswa kwemitholampilo yokutheleleka kwe-DENV ikakhulukazi kuhlanganisa ikhanda, imfiva, ubuthakathaka, ukwanda kwe-lymph node, i-leukopenia nokunye, nokopha, ukushaqeka, ukulimala kwesibindi noma ngisho nokufa ezimweni ezimbi kakhulu. Eminyakeni yamuva nje, ukuguquka kwesimo sezulu, ukufudukela kwabantu emadolobheni, ukuthuthuka okusheshayo kwezokuvakasha kanye nezinye izici zinikeze izimo ezisheshayo nezilula zokudlulisa nokusabalalisa i-DF, okuholela ekwandeni okuqhubekayo kwendawo ewubhubhane ye-DF.
Isiteshi
| FAM | I-DENV i-nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | -18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | I-serum entsha |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | 500 Amakhophi/mL |
| Ukucaciswa | Imiphumela yokuhlolwa kokugxambukela ibonisa ukuthi lapho ukugcwala kwe-bilirubin ku-serum kungengaphezu kuka-168.2μmol/ml, ukuhlushwa kwe-hemoglobin ekhiqizwa yi-hemolysis akukho ngaphezu kwe-130g/L, ukuhlushwa kwe-lipid yegazi akukho ngaphezu kuka-65mmol/ml, inani eliphelele le-IgG ku-serum alikho ngaphezu kuka-5mg/mL, akukho mphumela wokutholwa kwegciwane i-dengue, igciwane le-chikungue. Igciwane le-Hepatitis A, igciwane le-Hepatitis B, igciwane le-Hepatitis C, i-Herpes virus, i-Eastern equine encephalitis virus, i-Hantavirus, igciwane le-Bunya, igciwane le-West Nile kanye namasampula e-genomic serum yabantu akhethiwe ukuze kuhlolwe i-cross-reactivity, futhi imiphumela ibonisa ukuthi akukho ukusabela okuphambene phakathi kwale kit kanye namagciwane ashiwo ngenhla. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
I-TIANamp Virus DNA/RNA Kit (YDP315-R), futhi ukukhipha kufanele kwenziwe ngokuhambisana ngokuqinile nomyalelo wokusetshenziswa. Ivolumu yesampula ekhishiwe ingu-140μL, futhi ivolumu ye-lution enconyiwe ingu-60μL.
Inketho yesi-2.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HC30, HWTS-300) Macro & Micro-Test Med-Tech Co., Ltd., futhi ukukhishwa kufanele kwenziwe ngokuvumelana nomyalelo wokusetshenziswa. Ivolumu yesampula ekhishiwe ingu-200μL, futhi ivolumu ye-lution enconyiwe ingu-80μL.
