I-Enterovirus 71 (EV71)
Igama lomkhiqizo
I-HWTS-EV003- I-Entovirus 71 (EV71) I-Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Isifo sesandla sezandla-izinyawo-siyisifo esithathelwanayo esibangelwa ama-etoloviruse (eV). Njengamanje, izinhlobo eziyi-108 zama-serotypes zama-etoloviruses atholakele, ahlukaniswe ngamaqembu amane: a, B, C no-D. Lesi sifo sivela kakhulu ezinganeni ezingaphansi kweminyaka engu-5, futhi singadala ama-herpes ezandleni, izinyawo, umlomo kanye nezinye izingxenye. Inani elincane lezingane lizothuthukisa izinkinga ezifana neMyOCarditis, i-Pulmonary Edema, ne-Aseptic Meningoencephalitis.
Insungubezi
| -Yenza | Ev71 |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-9 |
| Uhlobo lwe-Specimen | I-Oropharyngengeal Swap,Uketshezi lwe-herpes |
| Ct | ≤35 |
| CV | <5.0% |
| Indlu edayisa insimbi | 500copies / ml |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-Lightcycler®480 izinhlelo ze-PCR zangempela zesikhathi sangempela I-LineGene 9600 Plus Real-Time Pcc Retection Systems I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1.
I-realent enconyelwe i-realent: I-Macro & Micro-Test Sampula Reled Reled Reagent (HWTS-3005-8), futhi ukukhishwa kufanele kwenziwe ngokuya ngomyalo wokusetshenziswa. Amasampula akhishwe yi-Oropharyngengeal Swabs noma ama-herpes fluid amasampula avela ezigulini eziqoqwe esizeni. Faka ama-swabs aqoqwe kwi-macro & micro-300 referent Reled Reled Reagent (HWTS-3005-8) ngqo, i-Vortex bese uxubana kahle, ukhiphe uphinde uhlanganise kahle, ukuthola i-RNA kwesampula ngayinye.
Inketho 2.
I-reaglent enconyiwe I-realent: Macro & Micro-Test General DNA / RNA KIT (HWTS-3017-50, HWS-3017-32, HWS-3017-48, HWS-3017-48, HWS-3017-46, HWS-3017-48, (i-RNOR-3017-96, (engasetshenziswa ngeMacro & Micro-Test I-automatic nucleic acid extractor (HWTS-3006C, HWS-3006B)) nguJiangsu IMacro & Micro-Test Med-Tech Co, Ltd., kanye nokukhishwa kufanele kwenziwe ngokuya ngomyalo wokusetshenziswa. Ivolumu yesampula ekhishwe i-200μL, futhi ivolumu yokuqedwa enconyiwe ingu-80μL.
Inketho3.
I-reaglent enconyiwe ye-realent: I-QAAMP Viral RNA MINI KIT (52904) nguQiagen noma i-Tianamp Virus DNA / RNA KIT (yppp15-R), futhi ukukhishwa kufanele kwenziwe ngokuqinile ngomyalo wokusetshenziswa. IVolumu yesampula ekhishwe i-140μL, kanti ivolumu yokuqedwa enconyiwe ingu-60μL.
