I-Enterovirus 71 (EV71)
Igama lomkhiqizo
HWTS-EV003- Enterovirus 71 (EV71) Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Hand-foot-mouth isifo isifo esithathelwanayo esibangelwa ama-enteroviruses (EV).Njengamanje, kutholakale izinhlobo ezingu-108 ze-serotypes ze-enteroviruses, ezihlukaniswe ngamaqembu amane: A, B, C no-D. Phakathi kwabo, i-enterovirus EV71 ne-CoxA16 yizifo eziyinhloko.Lesi sifo sivame ukuvela ezinganeni ezingaphansi kweminyaka engu-5 ubudala, futhi zingabangela i-herpes ezandleni, ezinyaweni, emlonyeni nakwezinye izingxenye.Inombolo encane yezingane izoba nezinkinga ezifana ne-myocarditis, i-pulmonary edema, ne-aseptic meningoencephalitis.
Isiteshi
| FAM | EV71 |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | I-Oropharyngeal swab,I-Herpes fluid |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500Amakhophi/mL |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), futhi ukukhishwa kufanele kwenziwe ngokuya ngomyalelo wokusetshenziswa.Amasampula akhishiwe ama-oropharyngeal swabs noma amasampula e-herpes fluid avela ezigulini eziqoqwe esizeni.Faka ama-swabs aqoqiwe ku-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) ngokuqondile, i-vortex bese uxuba kahle, beka endaweni efudumele imizuzu emi-5, uwakhiphe bese uwaguqula ukuze uxube kahle, ukuze uthole i-RNA. yesampula ngayinye.
Inketho yesi-2.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., futhi ukukhishwa kufanele kwenziwe ngokuvumelana nomyalelo wokusetshenziswa.Ivolumu yesampula ekhishiwe ingu-200µL, futhi ivolumu ye-elution enconyiwe ngu-80µL.
Inketho3.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-QIAamp Viral RNA Mini Kit (52904) nge-QIAGEN noma i-TIANamp Virus DNA/RNA Kit (YDP315-R), futhi ukukhipha kufanele kwenziwe ngokuhambisana ngokuqinile nomyalelo wokusetshenziswa.Ivolumu yesampula ekhishiwe ingu-140μL, futhi ivolumu ye-elution enconyiwe ingu-60µL.
