I-Enterovirus 71 (EV71)
Igama lomkhiqizo
Ikhithi Yokuthola I-Nucleic Acid ye-HWTS-EV003- Enterovirus 71 (EV71) (i-Fluorescence PCR)
I-Epidemiology
Isifo somlomo wesandla siyisifo esithathelwanayo esibangelwa ama-enterovirus (EV). Njengamanje, kutholakale izinhlobo ezingu-108 zama-serotypes ama-enterovirus, ahlukaniswe ngamaqembu amane: A, B, C kanye no-D. Phakathi kwawo, i-enterovirus EV71 kanye ne-CoxA16 yizona zifo eziyinhloko. Lesi sifo sivame ukwenzeka ezinganeni ezingaphansi kweminyaka emi-5 ubudala, futhi singadala i-herpes ezandleni, ezinyaweni, emlonyeni nakwezinye izingxenye. Inani elincane lezingane lizoba nezinkinga ezifana ne-myocarditis, i-pulmonary edema, kanye ne-aseptic meningoencephalitis.
Isiteshi
| I-FAM | I-EV71 |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | I-Oropharyngeal swab,Uketshezi lwe-Herpes |
| Ct | ≤35 |
| CV | <5.0% |
| I-LoD | Amakhophi angu-500/mL |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Izinhlelo ze-PCR zesikhathi sangempela ezingu-480 Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
I-reagent yokukhipha enconywayo: I-Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8), futhi ukukhishwa kufanele kwenziwe ngokwemiyalelo yokusetshenziswa. Amasampula akhishwe yi-oropharyngeal swabs noma amasampula e-herpes fluid avela ezigulini eziqoqwe endaweni. Faka ama-swabs aqoqwe ku-Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8) ngqo, uvortex bese uxuba kahle, ubeke ekamelweni lokushisa imizuzu emi-5, uwakhiphe bese uwaguqula ukuze uxube kahle, ukuze uthole i-RNA yesampula ngayinye.
Inketho 2.
I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., futhi ukukhipha kufanele kwenziwe ngokwemiyalelo yokusebenzisa. Ivolumu yesampula ekhishwe ingu-200µL, kanti ivolumu yokususa enconyiwe ingu-80µL.
Inketho 3.
I-reagent yokukhipha enconyiwe: I-QIAamp Viral RNA Mini Kit (52904) yi-QIAGEN noma i-TIANamp Virus DNA/RNA Kit (YDP315-R), futhi ukukhishwa kufanele kwenziwe ngokuhambisana ngqo nemiyalelo yokusebenzisa. Umthamo wesampula okhishwe ngu-140μL, kanti umthamo wokususa onconyiwe ngu-60μL.
