Ikhithi Yokuthola I-Herpes Simplex Virus Type 2 Nucleic Acid (i-Fluorescence PCR)

Ikhithi Yokuthola I-Herpes Simplex Virus Type 2 Nucleic Acid (Fluorescence PCR) Efakiwe

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola i-herpes simplex virus type 2 nucleic acid esezingeni eliphezulu kumasampula e-urethral swab yabesilisa kanye ne-cervical swab yabesifazane.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama Lomkhiqizo

Ikhithi Yokuthola I-Herpes Simplex Virus Type 2 Nucleic Acid (i-Fluorescence PCR)

Ukusetshenziswa Okuhlosiwe

Le khithi isetshenziselwa ukuthola i-herpes simplex virus type 2 nucleic acid esezingeni eliphezulu kumasampula e-urethral swab yabesilisa kanye ne-cervical swab yabesifazane.

I-Epidemiology

Uhlobo 2 lwegciwane i-Herpes Simplex (HSV2) luyigciwane eliyindilinga elenziwe nge-tegument, i-capsid, i-core, kanye ne-envelope, futhi liqukethe i-DNA eqondile enezintambo ezimbili. Igciwane le-Herpes lingangena emzimbeni ngokuxhumana okuqondile noma ngokuxhumana ngokocansi nesikhumba kanye nolwelwesi lwama-mucous, futhi lihlukaniswe libe yi-primary kanye ne-recurrent. Ukutheleleka komgudu wokuzala kubangelwa kakhulu yi-HSV2, iziguli zesilisa zibonakala njengezilonda zepenile, kanti iziguli zesifazane zibonakala njengezilonda zomlomo wesibeletho, ze-vulvar, nezesitho sangasese sowesifazane. Ukutheleleka kokuqala kwegciwane le-herpes lobulili ikakhulukazi izifo ezithathelwanayo, ngaphandle kwe-herpes ezimbalwa zasendaweni ezine-mucous membranes noma isikhumba, eziningi zazo ezingenazo izimpawu zomtholampilo ezisobala. Ukutheleleka kwe-herpes yobulili kunezici zokuthwala igciwane impilo yonke kanye nokuphinda kube lula, futhi iziguli kanye nabathwali balo bangumthombo wokutheleleka kwalesi sifo. E-China, izinga le-serological positive le-HSV2 lingaba ngu-10.80% kuya ku-23.56%. Isigaba sokutheleleka kwe-HSV2 singahlukaniswa sibe ukutheleleka okuyinhloko kanye nokutheleleka okuphindaphindayo, kanti cishe ama-60% eziguli ezine-HSV2 aphinde abuye.

I-Epidemiology

I-FAM: I-Herpes Simplex Virus Type 2 (HSV2)·

I-VIC(HEX): Ukulawula Kwangaphakathi

Ukusetha Izimo Zokukhulisa ze-PCR

Isinyathelo	Imijikelezo	Izinga lokushisa	Isikhathi	QoqaFi-luorescentSizibonakalisonoma cha
1	Umjikelezo ongu-1	50℃	imizuzu emi-5	No
2	Umjikelezo ongu-1	95℃	imizuzu eyi-10	No
3	Imijikelezo engu-40	95℃	imizuzwana eyi-15	No
4	Imijikelezo engu-40	58℃	amasekhondi angu-31	Yebo

Amapharamitha Obuchwepheshe

Isitoreji
Uketshezi	≤-18℃ Ebumnyameni
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	I-swab yomlomo wesibeletho yowesifazane, i-swab yomlomo wesibeletho yowesilisa
Ct	≤38
CV	≤5.0%
I-LoD	Amakhophi angu-50/ukusabela
Ukucaciswa	Akukho ukuhlangana kabusha namanye amagciwane e-STD, njenge-Treponema pallidum, i-Chlamydia trachomatis, i-Ureaplasma urealyticum, i-Mycoplasma hominis, i-Mycoplasma genitalium nokunye.
Izinsimbi Ezisebenzayo	Ingafanelana namathuluzi e-PCR e-fluorescent ajwayelekile emakethe. Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR.

Ukuhamba komsebenzi

Ikhithi Yokuthola I-Herpes Simplex Virus Type 2 Nucleic Acid (i-Fluorescence PCR)

Ama-reagent adinga kodwa awanikezwa

I-saline evamile, i-Macro kanye ne-Micro-Test Sample Release Release Reagent (HWTS-3005-8), i-Macro kanye ne-Micro-Test General DNA/RNA Kit (HWTS-3017) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B), i-Nucleic Acid Extraction noma i-Purification Reagent (YDP302) yi-Tiangen Biotech (Beijing) Co., Ltd.

Izinto ezisetshenziswayo ziyadingeka kodwa azinikezwa

I-1.5mL yeshubhu ye-centrifuge engena-DNase/RNase, i-DNase/RNase engena-Tip, i-ethanol ephelele, i-pipette, i-desktop centrifuge, ingxube ye-desktop oscillating.

Okwedlule: Ikhithi Yokuthola I-Neisseria Gonorrhoeae Nucleic Acid

Olandelayo: Ikhithi Yokuthola I-Chlamydia Trachomatis Nucleic Acid (i-Fluorescence PCR)

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