I-Herpes Simplex Virus Type 2 Nucleic Acid

Incazelo emfushane:

Le kit isetshenziselwa ukutholwa kwekhwalithi ye-herpes simplex virus uhlobo 2 lwe-nucleic acid ku-swab ye-urethral yabesilisa kanye namasampula womlomo wesibeletho wesifazane.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama Lomkhiqizo

HWTS-UR007A-Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Fluorescence PCR)

Ukusetshenziswa Okuhlosiwe

Le kit isetshenziselwa ukutholwa kwekhwalithi ye-herpes simplex virus uhlobo 2 lwe-nucleic acid ku-swab ye-urethral yabesilisa kanye namasampula womlomo wesibeletho wesifazane.

I-Epidemiology

I-Herpes Simplex Virus Type 2 (HSV2) igciwane eliyindilinga elihlanganiswe ne-tegument, capsid, core, nemvilophu, futhi liqukethe i-DNA yomugqa enemicu ekabili.I-Herpes virus ingangena emzimbeni ngokuthintana okuqondile noma ukuthintana ngokocansi nesikhumba kanye nolwelwesi lwamafinyila, futhi ihlukaniswe yaba okuyinhloko nokuphindaphindayo.Ukutheleleka emgudwini wokuzala kubangelwa ikakhulukazi i-HSV2, iziguli zesilisa zibonakala njengezilonda ze-penile, futhi iziguli zesifazane zibonakala njengezilonda zomlomo wesibeletho, i-vulvar, ne-vaginal.Izifo zokuqala zegciwane le-herpes ezithweni zangasese ngokuvamile ziyizifo eziphindaphindayo, ngaphandle kwe-herpes embalwa yendawo enolwelwesi lwamafinyila noma isikhumba, iningi lazo elingenazo izimpawu zomtholampilo ezisobala.Ukutheleleka kwe-herpes yangasese kunezici zokuthwala igciwane impilo yonke kanye nokuphindaphinda kalula, futhi kokubili iziguli nabathwali bawumthombo wokutheleleka kwalesi sifo.E-China, izinga le-serological positive le-HSV2 licishe libe ngu-10.80% kuya ku-23.56%.Isigaba sokutheleleka nge-HSV2 singahlukaniswa sibe ukutheleleka okuyisisekelo kanye nokutheleleka okuphindaphindayo, futhi cishe u-60% weziguli ezithelelekile ze-HSV2 ziyaphinda.

I-Epidemiology

I-FAM: I-Herpes Simplex Virus Type 2 (HSV2)·

I-VIC(HEX): Ukulawula Kwangaphakathi

 

Izilungiselelo ze-PCR Amplification Conditions

Isinyathelo

Imijikelezo

Izinga lokushisa

Isikhathi

QoqaFi-luorescentSama-ignalsnoma cha

1

1 Umjikelezo

50℃

5 imiz

No

2

1 Umjikelezo

95℃

10 imiz

No

3

40 imijikelezo

95℃

15 amasekhondi

No

4

58℃

31 isekhondi

Yebo

Imingcele Yezobuchwepheshe

Isitoreji  
Uketshezi

≤-18℃ ebumnyameni

Impilo yeshelufu Izinyanga ezingu-12
Uhlobo lwesifanekiso

I-swab yomlomo wesibeletho wesifazane, I-swab ye-urethral yesilisa

Ct

≤38

CV

≤5.0%

LoD 50Amakhophi/ukusabela
Ukucaciswa

Akukho ukuhlangana kabusha namanye amagciwane e-STD, njenge-Treponema pallidum, i-Chlamydia trachomatis, i-Ureaplasma urealyticum, i-Mycoplasma hominis, i-Mycoplasma genitalium nokunye.

Izinsimbi Ezisebenzayo

Ingakwazi ukufanisa amathuluzi we-PCR we-fluorescent avamile emakethe.

I-Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

I-QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

I-LineGene 9600 Plus Real-Time PCR Detection System

I-MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Real-Time PCR System

I-BioRad CFX Opus 96 Real-Time PCR System.

Ukugeleza komsebenzi

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